結(jié)合緩釋微球的ZK60鎂合金支架應(yīng)用于骨再生的研究
發(fā)布時間：2018-07-13 14:45
【摘要】：骨修復過程是一個受多種生長因子影響的過程,這個過程中,多種因子的時空分布,對骨修復的成功率和速率起關(guān)鍵作用。利用組織工程的方法修復骨缺損是研究的熱點,也取得了很多的進展,然而當前的骨支架材料只結(jié)合了單一的活性因子,或者多個因子的簡單混合,未能模擬骨自然修復過程中多因子的時空分布,從而影響了治療方法的效率。因此,兩種或多種因子的延時控制釋放體系的研究是很有意義的。靜電噴霧技術(shù)是一種利用強靜電場作用來克服液體表面張力,從而獲得微球的一種技術(shù)。利用同軸靜電噴霧法可以制備殼-核結(jié)構(gòu)的高分子微球,這種高分子微球能依時間順序先后釋放兩種化學藥物,如前1-2周釋放一種藥物,之后開始釋放第二種藥物。通過變化殼-核結(jié)構(gòu)中核的相對大小,能精確控制兩種藥物的釋放節(jié)奏。因此,可以利用同軸靜電噴霧技術(shù)制備殼-核分別包含F(xiàn)GF-2和BMP-2這兩種生長因子的微球,從而實現(xiàn)因子的延時控制釋放,達到促進骨再生的目的。殼-核結(jié)構(gòu)高分子微球,雖然有一定機械性能,但不能滿足骨缺損部位,特別是受力部位的支撐作用,從而影響骨再生。ZK60鎂合金具有與自然骨接近的機械性能,而其降解產(chǎn)物鎂離子又是人體必需元素。所以,可以利用鎂合金作為殼-核微球的外部支撐材料,從而滿足骨再生的要求。但鎂合金過快的降解速度影響了其應(yīng)用,所以必需對其進行表面改性,延緩其降解速率。Ca和P都是骨基質(zhì)的主要組成成分,研究結(jié)果顯示Ca-P涂層具有良好的生物相容性,并有很好的骨傳導性和誘骨活性。所以可以在ZK60鎂合金表面進行Ca-P涂層,這樣既可以延緩降解速率,又可以促進骨再生。本實驗通過制備搭載包含兩種生長因子的可控釋放微球的Ca-P改性ZK60鎂合金支架,即可以發(fā)揮殼-核結(jié)構(gòu)微球的蛋白質(zhì)緩釋方面的優(yōu)勢,又可以在骨缺損處的應(yīng)用提供機械支撐,從而提高修復大面積骨缺損的效率。
[Abstract]:The process of bone repair is a process influenced by many growth factors. In this process, the spatial and temporal distribution of multiple factors plays a key role in the success rate and rate of bone repair. The application of tissue engineering in repairing bone defects is a hot topic and has made a lot of progress. However, the current bone scaffold materials only combine a single active factor or a simple mixture of multiple factors. The failure to simulate the temporal and spatial distribution of multiple factors in the process of natural bone repair affects the efficiency of the treatment. Therefore, the study of delay controlled release systems with two or more factors is of great significance. Electrostatic spray technology is a kind of technology which uses the strong electrostatic field to overcome the surface tension of liquid and obtain microspheres. Coaxial electrostatic spray method can be used to prepare shell-core polymer microspheres which can release two kinds of chemical drugs in order of time such as the release of one drug in the first 1-2 weeks and then the release of the second drug. By changing the relative size of the nuclei in the shell-nucleus structure, the release rhythm of the two drugs can be precisely controlled. Therefore, the coaxial electrostatic spray technique can be used to prepare the microspheres containing FGF-2 and BMP-2, respectively, so that the delayed release of the factors can be achieved and the bone regeneration can be promoted. Although the shell core structure polymer microspheres have some mechanical properties, they can not meet the support function of bone defect, especially the bearing parts, thus affecting the mechanical properties of bone regeneration. ZK60 magnesium alloy has the mechanical properties close to that of natural bone. Mg ~ (2 +), the degradation product of magnesium ion, is an essential element in human body. Therefore, magnesium alloy can be used as an external support material for shell-nuclear microspheres to meet the requirements of bone regeneration. However, the rapid degradation rate of magnesium alloy affects its application, so it is necessary to modify its surface and delay its degradation rate. Ca and P are the main components of bone matrix. The results show that Ca-P coating has good biocompatibility. It also has good bone conduction and osteoinductive activity. Therefore, Ca-P coating can be applied on ZK60 magnesium alloy surface, which can delay degradation rate and promote bone regeneration. In this experiment, Ca-P modified ZK60 magnesium alloy scaffolds with controllable release microspheres containing two growth factors were prepared, which can play the advantage of protein sustained release of shell core microspheres and provide mechanical support for bone defects. So as to improve the efficiency of repairing large-area bone defects.
【學位授予單位】：湖南大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R318.08;R68
[Abstract]:The process of bone repair is a process influenced by many growth factors. In this process, the spatial and temporal distribution of multiple factors plays a key role in the success rate and rate of bone repair. The application of tissue engineering in repairing bone defects is a hot topic and has made a lot of progress. However, the current bone scaffold materials only combine a single active factor or a simple mixture of multiple factors. The failure to simulate the temporal and spatial distribution of multiple factors in the process of natural bone repair affects the efficiency of the treatment. Therefore, the study of delay controlled release systems with two or more factors is of great significance. Electrostatic spray technology is a kind of technology which uses the strong electrostatic field to overcome the surface tension of liquid and obtain microspheres. Coaxial electrostatic spray method can be used to prepare shell-core polymer microspheres which can release two kinds of chemical drugs in order of time such as the release of one drug in the first 1-2 weeks and then the release of the second drug. By changing the relative size of the nuclei in the shell-nucleus structure, the release rhythm of the two drugs can be precisely controlled. Therefore, the coaxial electrostatic spray technique can be used to prepare the microspheres containing FGF-2 and BMP-2, respectively, so that the delayed release of the factors can be achieved and the bone regeneration can be promoted. Although the shell core structure polymer microspheres have some mechanical properties, they can not meet the support function of bone defect, especially the bearing parts, thus affecting the mechanical properties of bone regeneration. ZK60 magnesium alloy has the mechanical properties close to that of natural bone. Mg ~ (2 +), the degradation product of magnesium ion, is an essential element in human body. Therefore, magnesium alloy can be used as an external support material for shell-nuclear microspheres to meet the requirements of bone regeneration. However, the rapid degradation rate of magnesium alloy affects its application, so it is necessary to modify its surface and delay its degradation rate. Ca and P are the main components of bone matrix. The results show that Ca-P coating has good biocompatibility. It also has good bone conduction and osteoinductive activity. Therefore, Ca-P coating can be applied on ZK60 magnesium alloy surface, which can delay degradation rate and promote bone regeneration. In this experiment, Ca-P modified ZK60 magnesium alloy scaffolds with controllable release microspheres containing two growth factors were prepared, which can play the advantage of protein sustained release of shell core microspheres and provide mechanical support for bone defects. So as to improve the efficiency of repairing large-area bone defects.
【學位授予單位】：湖南大學
【學位級別】：碩士
【學位授予年份】：2015
【分類號】：R318.08;R68
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1 王學俠;以硫酸鈣屏障行骨再生[J];國外醫(yī)學.口腔醫(yī)學分冊;1998年04期
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6 郭立宏;;骨再生膠囊治療骨折延遲愈合32例[J];北京中醫(yī)藥大學學報(中醫(yī)臨床版);2008年04期
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8 肖平;大型下頜骨囊腫摘除術(shù)后的自行骨再生[J];國外醫(yī)學.口腔醫(yī)學分冊;2001年06期
9 ;新技術(shù)可促血管生成及骨再生[J];山東中醫(yī)藥大學學報;2010年06期
10 劉守應(yīng);王繼芳;蔡，
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