本文選題：肝損傷 + Neuritin蛋白�。� 參考：《石河子大學(xué)》2017年碩士論文

【摘要】：目的:通過SD大鼠肝損傷造模來探討大鼠肝損傷過程中神經(jīng)突蛋白(Neuritin)在肝臟組織中隨著不同時限的表達(dá),根據(jù)其含量的變化來判斷神經(jīng)突蛋白(Neuritin)在肝臟損傷后的意義,為深入研究肝細(xì)胞再生與肝臟系統(tǒng)可塑性的分子機制奠定理論基礎(chǔ);為肝細(xì)胞損傷后的再生修復(fù)研究提供新思路。方法:由新疆醫(yī)科大學(xué)動物飼養(yǎng)中心提供的6周齡、清潔級的SD大鼠126只隨機分三組,空白組、非肝損傷組和肝損傷組各組42SD大鼠,空白組不給任何處理,非肝損傷組在大鼠股骨處同一部位同一力度致大鼠股骨骨折,肝損傷組使用自制改良BIM-IV型生物撞擊建立大鼠肝損傷模型,模型建立后分成6h,12h,1d,2d,3d,5d共6個亞組分別處死大鼠,再經(jīng)HE染色、免疫組化化學(xué)染色并檢測血清中丙氨酸轉(zhuǎn)氨酶(ALT)、天冬氨酸轉(zhuǎn)氨酶(AST),觀察各個時間點肝臟組織中Neuritin變化及表達(dá)水平。結(jié)果:根據(jù)造模后SD大鼠血清中ALT、AST顯示:肝損傷組血清中ALT、AST 6h時開始升高,12h小時升高達(dá)到最高值,1d-2d時開始下降但仍高于空白組、非肝損傷組(P0.01),3d-5d時趨向正常水平。免疫組化示:肝損傷組neuritin蛋白6 h呈陽性表達(dá),12 h呈強陽性,1d-3d呈弱陽性表達(dá),但仍高于空白組與對照組(P0.01),5d時肝損傷組neuritin的表達(dá)趨向正常水平。HE染色示:空白組和非肝損傷組肝小葉結(jié)構(gòu)清楚,沒有明顯損傷。肝損傷組肝細(xì)胞,出現(xiàn)明顯氣球樣變,肝細(xì)胞大片狀壞死甚至出現(xiàn)細(xì)胞核脫失,細(xì)胞壞死。結(jié)論:1.本實驗證明在大鼠肝損傷后均引起肝組織中Neuritin的表達(dá)量增加并且與大鼠肝臟損傷程度變化有一定關(guān)系。2.通過本研究發(fā)現(xiàn)神經(jīng)營養(yǎng)因子Neuritin在大鼠肝損傷細(xì)胞周圍有很高的表達(dá),和肝損傷血清中ALT、AST水平變化一致,由此推斷Neuritin可能在肝細(xì)胞的再生修復(fù)或增殖過程中起著重要作用。
[Abstract]:Objective: to investigate the expression of neurite protein Neuritinin in rat liver tissue with different time limits, and to determine the significance of neurite protein Neuritin after liver injury according to the changes of its content.It provides a theoretical basis for the further study of the molecular mechanism of hepatocyte regeneration and liver system plasticity, and provides a new idea for the regeneration and repair of liver cells after injury.Methods: a total of 126 SD rats, aged 6 weeks, provided by the Animal feeding Center of Xinjiang Medical University, were randomly divided into three groups: blank group, non-hepatic injury group and liver injury group.In the non-hepatic injury group, the femur fracture was induced by the same force at the same position of the femur of the rats. The liver injury group established the rat liver injury model by using self-made modified BIM-IV biological impact. After the establishment of the model, the rats were divided into 6 subgroups, 6 subgroups were divided into 6 subgroups respectively.After HE staining and immunohistochemical staining, serum alanine aminotransferase (alt) and aspartate transaminase (AST) were detected. The changes and expression of Neuritin in liver tissue were observed at different time points.Results: according to the alt AST in the serum of SD rats after model making, the alt AST in the serum of the liver injury group began to rise at 6 h and reached the highest value at 12 h. It began to decrease but was still higher than that of the blank group at 1 d ~ 2 d. The level of alt AST in the serum of the non-hepatic injury group reached normal level at 3 d ~ 5 d.Immunohistochemistry showed that the expression of neuritin protein in liver injury group was strongly positive at 6 h and strong positive at 12 h. The expression of neuritin protein was weak at 1 d ~ 3 d after liver injury.However, the expression of neuritin in liver injury group was higher than that in blank group and control group for 5 days. The results of HE staining showed that the structure of hepatic lobules in blank group and non-hepatic injury group was clear and no obvious damage was found.In the group of liver injury, there were obvious balloon degeneration, large slice necrosis and even loss of nucleus and necrosis of the cells.Conclusion 1.This study demonstrated that the expression of Neuritin increased after liver injury in rats and was related to the degree of liver injury in rats.It was found that the expression of neurotrophic factor Neuritin was very high around the rat liver injury cells, which was consistent with the changes of alt AST level in the serum of liver injury. It was concluded that Neuritin may play an important role in the regeneration and proliferation of hepatocytes.
【學(xué)位授予單位】：石河子大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R657.3

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