本文選題：痙攣 + 肉毒毒素　； 參考：《中國康復(fù)醫(yī)學(xué)雜志》2017年07期

【摘要】：目的:探討在不同時間點進行A型肉毒毒素(BoNT-A)注射干預(yù)對脊髓損傷(SCI)大鼠肌張力(MAS)、運動功能狀態(tài)(BBB)及腓腸肌(GM)病理特征的影響,進而尋找BoNT-A干預(yù)痙攣最佳時間的理論依據(jù)。方法:48只SD雄性大鼠被隨機分配至Nor-Contrl組、12周-Contrl組、NS干預(yù)組和BT干預(yù)組。NS/BT干預(yù)組按干預(yù)時間又分設(shè)3個亞組(2周-NS亞組、2周-BT亞組、4周-NS亞組、4周-BT亞組、8周-NS亞組、8周-BT亞組)。Nor-Contrl組和12周-Contrl組大鼠不予注射藥物;BT/NS干預(yù)組,按上述時間點分別給予BoNT-A/生理鹽水注射大鼠右GM。肌張力評估采用MAS評估,運動功能采用BBB評分。GM標(biāo)本進行肌重測量及蛋白電泳分析(MyHC)。結(jié)果:與12周-Contrl組相比,BT干預(yù)組大鼠GM肌重明顯下降(P≤0.05)。與12周-Contrl組相比,干預(yù)組大鼠MAS及BBB結(jié)果差異無顯著性意義(P0.05)。BT干預(yù)組中不同亞組大鼠GM的MyHC分型占比較Nor-Contrl組及12周-Contrl組間的差異均有顯著性意義(P≤0.05);且隨著干預(yù)時間點不同,BT干預(yù)組大鼠GM的MyHC分型占比亦不同。結(jié)論:BoNT-A注射干預(yù)導(dǎo)致受注射的GM肌重及肌重/體質(zhì)量比顯著下降,萎縮明顯;對受試大鼠MAS及BBB無明顯影響;早期注射較后期注射更易引起GM發(fā)生MyHC構(gòu)型改變。
[Abstract]:Objective: to investigate the effects of botulinum toxin A (BoNT-A) injection at different time points on the pathological characteristics of muscular tension muscle-motility and gastrocnemius in Spinal Cord injury (sci) rats, and to find the theoretical basis for the best time of BoNT-A intervention in spasm.Methods Forty-eight Sprague-Dawley male rats were randomly assigned to Nor-Contrl group (n = 12) -Contrl group (n = 12) and BT intervention group (n = 10). NSP-BT intervention group was divided into three subgroups according to the time of intervention: 2 weeks, 2 weeks, 2 weeks, 4 weeks, 4 weeks, 4 weeks, 4 weeks, 4 weeks, 8 weeks, NS, respectively.The rats in the 8-week BT subgroup, Nor-Contrl group and 12-week-Contrl group were not injected with BT- / NS.According to the above time points, the rats were injected with BoNT-A/ saline respectively.The muscle tension was assessed by MAS, and the muscle weight was measured by BBB score. The protein electrophoresis was used to analyze MyHCM.Results: compared with 12 weeks-Contrl group, the weight of GM muscle decreased significantly in BT intervention group (P 鈮，

本文編號：1763460