本文選題：移植物血管病 + 移植��； 參考：《中國(guó)比較醫(yī)學(xué)雜志》2017年05期

【摘要】：目的移植物血管病(allograft vasculopathy,AV)限制著移植器官功能及遠(yuǎn)期存活,是器官移植領(lǐng)域最迫切需要解決的世界性難題。傳統(tǒng)的血管縫合方式需要優(yōu)秀的顯微外科技術(shù),開(kāi)展困難。探索出更為簡(jiǎn)便、可行的動(dòng)脈移植方法,可為臨床研究AV提供思路。方法采用靜脈留置針管自制血管套管,取供體大鼠胸主動(dòng)脈牽拉入套管并兩端翻轉(zhuǎn)、結(jié)扎固定為移植體,將該移植體植入受體大鼠腹主動(dòng)脈并于造模后1周、4周、8周取移植段動(dòng)脈行HE染色,觀測(cè)移植血管形態(tài)、測(cè)量?jī)?nèi)膜厚度。結(jié)果 40只受體大鼠中有38只健康存活達(dá)所需檢測(cè)時(shí)間點(diǎn),下肢及尾巴活動(dòng)自如、大小便正常,且HE染色可見(jiàn)移植段血管內(nèi)膜表現(xiàn)為與AV一致的同心圓樣增厚。結(jié)論此種大鼠動(dòng)脈移植方法簡(jiǎn)便可行,較傳統(tǒng)血管縫合方法可明顯縮短腹主動(dòng)脈阻斷時(shí)間、減輕受體大鼠的手術(shù)創(chuàng)傷、造模成功率高,可復(fù)制性強(qiáng)。
[Abstract]:Objective allograft vasculopathy AVV (allograft vasculopathy AVV) restricts organ function and long-term survival, which is the most urgent problem in the field of organ transplantation.Traditional vascular suture requires excellent microsurgical techniques and is difficult to develop.To explore a more convenient and feasible method of arterial transplantation, which can provide ideas for clinical study of AV.Methods the venous indwelling needle tube was used to make the vascular cannula. The donor thoracic aorta was pulled into the cannula and the two ends were flipped over and ligated and fixed into the graft.The graft was implanted into the abdominal aorta of the recipient rats. The grafts were stained with HE at the end of 4 weeks and 8 weeks after the model. The morphology of the grafts was observed and the thickness of the intima was measured.Results 38 of the 40 recipient rats survived to the required time point, the lower extremities and tails moved freely, the urine and the feces were normal, and HE staining showed that the intima of the grafts showed concentric circular thickening consistent with that of AV.Conclusion this method is simple and feasible. Compared with the traditional vascular suture method, it can significantly shorten the time of abdominal aorta occlusion, alleviate the surgical trauma of the recipient rats, and have a high success rate and high reproducibility.
【作者單位】： 三峽大學(xué)第一臨床醫(yī)學(xué)院;宜昌市中心人民醫(yī)院胸心大血管外科三峽大學(xué)心血管病研究所;
【分類(lèi)號(hào)】：R-332;R654.3

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