本文選題：骨生長(zhǎng)發(fā)育　切入點(diǎn)：DON毒素　出處：《中國(guó)地方病防治雜志》2017年05期

【摘要】：目的觀察DON毒素和氟對(duì)TGF-β2等4種骨生長(zhǎng)發(fā)育相關(guān)指標(biāo)的影響,探討DON毒素聯(lián)合元素氟致骨軟骨的損傷機(jī)制。方法 28日齡Wistar大鼠40只,按照隨機(jī)化方法分為空白組、氟(F)組、DON毒素組和"DON+F"組。F組和"DON+F"組飲用150 mg/L Na F溶液,空白組和DON毒素組飲用去離子水;各組的DON毒素染毒按0.2μg/g劑量每天灌胃,空白組和F組用等量蒸餾水灌胃。喂養(yǎng)5周后取血,ELISA法測(cè)定各細(xì)胞因子。結(jié)果血清中TGF-β2的平均含量"DON+F"組(55.47±5.07 pg/ml)DON組(38.88±7.99 pg/ml)F組(38.29±5.61 pg/ml)對(duì)照組(29.20±6.12 pg/ml);血清中BMP-2的平均含量"DON+F"組(15.24±0.66 ng/ml)F組(14.70±1.88 ng/ml)DON組(13.94±0.70 ng/ml)對(duì)照組(12.44±1.07 ng/ml);血清中Hyp的平均含量"DON+F"組(7.30±1.20μg/ml)DON組(7.38±1.65μg/ml)F組(8.35±1.51μg/ml)對(duì)照組(12.51±1.79μg/ml);血清中TNF-α的平均含量"DON+F"組(936.88±126.55 pg/ml)F組(860.09±117.45 pg/ml)DON組(706.52±73.72 pg/ml)對(duì)照組(579.54±144.53pg/ml)。結(jié)論 DON毒素和F存在一定的交互作用,可導(dǎo)致染毒大鼠血清TGF-β2等細(xì)胞因子的變化,進(jìn)而影響骨軟骨的生長(zhǎng)發(fā)育,導(dǎo)致病理?yè)p傷。
[Abstract]:Objective to observe the effects of DON toxin and fluorine on four related indexes of bone growth and development, such as TGF- 尾 2, and to explore the mechanism of bone cartilage injury induced by DON toxin and fluoride. Methods Forty Wistar rats aged 28 days were randomly divided into blank group. Don toxin group, "DON F" group and "DON F" group drank 150 mg/L NaF solution, blank group and DON toxin group drank deionized water. The serum TGF- 尾 2 was measured by Elisa after 5 weeks of feeding. Results the average content of TGF- 尾 2 in serum of "DON F" group was 55.47 鹵5.07 pg/ml)DON group (38.88 鹵7.99 pg/ml)F) control group (29.20 鹵6.12 PG / ml) and control group (29.20 鹵6.12 PG / ml), and the average content of BMP-2 in serum was "DON F" in the control group (29.20 鹵6.12 PG / ml). The average content of TNF- 偽 in the control group was 12.44 鹵1.07 ng / ml, in the control group was 12.44 鹵1.07 ng / ml, in the "DON F" group was 7.30 鹵1.20 渭 g / ml, in the "DON F" group was 7.30 鹵1.65 渭 g/ml)F group (8.35 鹵1.51 渭 g / ml), in the control group was 12.51 鹵1.79 渭 g / ml / ml, and in the DON F group was 936.88 鹵126.55 pg/ml)F group (866.52 鹵73.72 PG / ml). Conclusion there is a certain interaction between DON and F in the control group. It can cause the change of TGF- 尾 2 and other cytokines in the serum of exposed rats, and then affect the growth and development of bone cartilage and lead to pathological injury.
【作者單位】： 泰山醫(yī)學(xué)院公共衛(wèi)生學(xué)院山東省醫(yī)藥衛(wèi)生流行病學(xué)重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金課題(編號(hào):81060228,81273155)
【分類(lèi)號(hào)】：R684.1

【相似文獻(xiàn)】

相關(guān)期刊論文 前1條

1 何雪梅,陳正清;四起進(jìn)食玉米或小麥粉引起脫氧雪腐鐮刀菌烯醇(DON)中毒調(diào)查報(bào)告(摘要)[J];廣西醫(yī)學(xué);1990年03期

相關(guān)碩士學(xué)位論文 前1條

1 李金平;DON致大骨節(jié)病發(fā)病機(jī)制研究[D];泰山醫(yī)學(xué)院;2011年

，

本文編號(hào)：1699267