發(fā)布時(shí)間：2018-03-30 12:11

  本文選題：膝關(guān)節(jié)　切入點(diǎn)：骨性關(guān)節(jié)炎　出處：《重慶醫(yī)學(xué)》2017年20期

【摘要】：目的探討富血小板血漿(PRP)對(duì)膝關(guān)節(jié)骨性關(guān)節(jié)炎(KOA)的治療機(jī)制及作用。方法取成年清潔級(jí)新西蘭大白兔32只,分為空白對(duì)照組(A組)、模型對(duì)照組(B組)、PRP治療組(C組)、玻璃酸鈉治療組(D組)。A組行模擬造模,B、C、D組按Hulth方法誘發(fā)建立兔KOA動(dòng)物模型。動(dòng)物模型建立成功后,C組予PRP 0.5mL關(guān)節(jié)腔注射治療,3周1次,共2次;D組予玻璃酸鈉注射液關(guān)節(jié)腔注射,每周1次,連續(xù)5周;A、B組于C組同一時(shí)間點(diǎn)給予等量無菌生理鹽水注射。在普通光學(xué)顯微鏡下觀察關(guān)節(jié)軟骨的組織結(jié)構(gòu)、細(xì)胞數(shù)量、潮線的完整性和甲苯胺藍(lán)染色等情況,比較各組間Mankin′s評(píng)分的差異。收集適量關(guān)節(jié)液測(cè)定各組花生四烯酸的表達(dá)情況。結(jié)果 A組關(guān)節(jié)軟骨結(jié)構(gòu)形態(tài)正常,無明顯破壞;B、C、D組關(guān)節(jié)軟骨結(jié)構(gòu)形態(tài)均有不同程度的破壞,尤以B組軟骨結(jié)構(gòu)改變較大,C、D組軟骨形態(tài)結(jié)構(gòu)雖有改變,但與A組軟骨結(jié)構(gòu)較接近。A組Mankin′s評(píng)分最低,B組評(píng)分最高,C、D組經(jīng)干預(yù)治療后評(píng)分明顯降低;B組與C、D組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05),C組與D組比較,差異有統(tǒng)計(jì)學(xué)意義(P0.05)。A組花生四烯酸水平最低,B組最高,C、D組水平介于A、B組之間,且C組低于D組(P0.05)。結(jié)論 PRP對(duì)KOA具有較為明顯的治療及緩解作用。
[Abstract]:Objective to investigate the therapeutic mechanism and effect of platelet-rich plasma (PRP) on osteoarthritis of knee joint (KOA).Methods Thirty-two adult clean grade New Zealand white rabbits were divided into three groups: control group A, model control group B and sodium hyaluronate treated group C, and sodium hyaluronate treated group D and group A were used to establish KOA animal model according to Hulth method.After the successful establishment of the animal model, group C was treated with intraarticular injection of PRP 0.5mL once for 3 weeks, group D was given intraarticular injection of sodium hyaluronate once a week, once a week, and group B was injected with the same amount of aseptic saline at the same time point in group C for 5 weeks.The structure of articular cartilage, the number of cells, the integrity of tidal line and toluidine blue staining were observed under general optical microscope, and the differences of Mankin's scores were compared among the three groups.The expression of arachidonic acid in each group was determined by collecting proper amount of joint fluid.Results the morphology of articular cartilage structure in group A was normal, and there was no obvious damage to the cartilage structure in group D, especially in group B, the cartilage structure of group C was changed more than that of group C, but the morphological structure of cartilage in group C was changed.However, the lowest Mankin's score in group A was lower than that in group A, and the highest score in group B was significantly lower than that in group C and group D after intervention. The difference was statistically significant between group C and group D.The level of arachidonic acid in group A was lower than that in group B and the highest level in group C was between group A and group A, and the level in group C was lower than that in group D (P 0.05).Conclusion PRP has obvious therapeutic and remission effects on KOA.
【作者單位】： 成都體育學(xué)院;
【基金】：四川省科技廳重點(diǎn)項(xiàng)目(2016JY0052);四川省科技廳支撐項(xiàng)目(2014SZ0003) 成都體育學(xué)院碩士點(diǎn)建設(shè)基金項(xiàng)目(16SSZX01)
【分類號(hào)】：R684.3

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