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  本文選題：組織工程　切入點(diǎn)：生物相容性材料　出處：《中國組織工程研究》2017年26期

【摘要】：背景:β磷酸三鈣和α半水硫酸鈣備的人工復(fù)合骨具有多孔顆粒形態(tài),生物相容性較高,用于脊柱融合模型中有助于融合率的提高,但該結(jié)論尚未得到證實。目的:觀察β磷酸三鈣/α半水硫酸鈣復(fù)合人工骨的制備方法、生物相容性及在脊柱融合模型中的應(yīng)用效果。方法:(1)將二水硫酸鈣在定點(diǎn)的條件、溫度下脫水制備α半水硫酸鈣;取健康牛松質(zhì)骨經(jīng)脫細(xì)胞、脫脂后在特點(diǎn)的條件、溫度下煅燒制備β磷酸三鈣多孔顆粒,溶于無水乙醇中,混懸,烘干后制備β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨。取購置的兔骨膜成骨細(xì)胞與復(fù)合材料共同培養(yǎng),觀察細(xì)胞形態(tài)、黏附能力及增殖活力;(2)取新西蘭大白兔30只,建立雙側(cè)新西蘭大白兔胸椎多節(jié)段后外側(cè)脊柱融合模型,左側(cè)植入β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨,右側(cè)植入自體骨進(jìn)行對照,比較2組融合率變化。結(jié)果與結(jié)論:(1)細(xì)胞形態(tài):相差顯微鏡觀察可見,L929系細(xì)胞株培養(yǎng)3 d后在β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨上貼壁數(shù)量相對較少;培養(yǎng)5 d后細(xì)胞在人工骨上貼壁相對密集;掃描電鏡下β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨表面存在許多結(jié)晶顆粒,表面吸附細(xì)胞相對較多;(2)β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨植入后4周融合率,高于自體骨(P0.05);(3)自體骨植入后4周組織切片中骨側(cè)小梁稀疏雜亂,可見新生的骨組織,無核的自體骨移植骨塊占據(jù)主導(dǎo)地位;融合后8周自體骨移植骨周圍新生骨組織進(jìn)一步增多;β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨植入后4周材料結(jié)構(gòu)清晰可見,未見降解碎片,內(nèi)部存在少許新生骨組織;植入后8周材料被新生骨組織包圍,骨小梁增粗,人工骨開始降解;(4)結(jié)果證實:制備的β磷酸三鈣和α半水硫酸鈣復(fù)合人工骨用于脊柱融合模型中能獲得較高的融合率。
[Abstract]:Background: the artificial composite bone prepared by 尾 tricalcium phosphate and 偽 -hemihydrate calcium sulfate has porous particle shape and high biocompatibility. It is helpful to improve the fusion rate in spinal fusion model, but this conclusion has not been confirmed.Objective: to observe the preparation method, biocompatibility and application effect of 尾 tricalcium phosphate / 偽 hemihydrate calcium sulfate composite artificial bone in spinal fusion model.Methods (1) calcium sulfate dihydrate was dehydrated at a fixed point to prepare 偽 -hemihydrate calcium sulfate, and healthy bovine cancellous bone was removed from cells, then calcined at temperature to prepare 尾 -tricalcium phosphate porous particles, which were dissolved in anhydrous ethanol.The composite artificial bone of 尾 -tricalcium phosphate and 偽 -hemihydrate calcium sulfate was prepared by suspension and drying.尾 tricalcium phosphate and 偽 -hemihydrate calcium sulfate composite artificial bone were implanted on the left side and autogenous bone was implanted on the right side to compare the fusion rate between the two groups.Results and conclusion: the cell morphology was observed by phase contrast microscope. After 3 days of culture, the number of adherent cells on 尾 tricalcium phosphate and 偽 -hemihydrate calcium sulfate composite artificial bone was relatively small, and the cell adhesion on artificial bone was relatively dense after 5 days of culture.4 weeks after autologous bone implantation, the bone trabeculae were sparse and disorganized. The new bone tissue was found, and the nucleless autologous bone grafts occupied the dominant position.8 weeks after implantation, the materials were surrounded by new bone tissue, the trabecular bone thickened, and the artificial bone began to degrade. The results showed that the 尾 -tricalcium phosphate and 偽 -hemihydrate calcium sulfate composite artificial bone could obtain high fusion rate in spinal fusion model.
【作者單位】： 海南醫(yī)學(xué)院第一附屬醫(yī)院骨科;
【基金】：海南省自然科學(xué)基金面上項目(20168297)~~
【分類號】：R318.08;R68

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