發(fā)布時(shí)間：2018-03-18 13:12

  本文選題：3D打印　切入點(diǎn)：骨組織工程　出處：《暨南大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：據(jù)報(bào)道,中國每年因?yàn)樽匀粸?zāi)害、交通事故、運(yùn)動(dòng)損傷等意外造成骨缺損的人數(shù)高達(dá)300到400萬人次,骨組織缺損給患者的日常生活帶來立巨大的不便。骨移植是解決骨缺損問題最有效的方法之一,眾多的患者需要進(jìn)行骨修復(fù)和骨移植治療。導(dǎo)致國內(nèi)外骨修復(fù)材料市場的需求日益增加。本研究工作以生物相容性良好的聚己內(nèi)酯(PCL)和季擕鹽(ATPB)、牡蠣殼粉(OSP)為原料,利用3D打印技術(shù)分別制備出具有優(yōu)良成骨效果的聚己內(nèi)酯/牡蠣殼粉骨組織工程支架和具有抗菌效果聚己內(nèi)酯/季擕鹽/牡蠣殼粉抗菌骨組織工程支架,并采用SEM、FTIR、DSC、力學(xué)試驗(yàn)、CCK-8檢測以及ALP檢測等多種測試表征方法對支架的物理化學(xué)性能和生物相容性進(jìn)行評價(jià),得出的結(jié)論如下:(1)通過對雙螺桿擠出機(jī)以及造粒機(jī)的參數(shù)進(jìn)行多次試驗(yàn)后,采用熔融共混擠出方法將粉末狀的牡蠣殼粉與聚己內(nèi)酯顆粒充分混合并擠出造粒,再經(jīng)由3D打印線材專用擠出機(jī)制得聚己內(nèi)酯/牡蠣殼粉和聚己內(nèi)酯/季擕鹽/牡蠣殼粉抗菌3D打印線材,所制得的3D打印線材粗細(xì)均勻,直徑為1.75±0.2mm,最佳打印溫度為150℃,打印流暢,擠料均勻。(2)利用3D打印技術(shù)制得的聚己內(nèi)酯/牡蠣殼粉骨組織工程支架具有三維連通的多孔結(jié)構(gòu),SEM、FTIR、DSC、CCK-8檢測以及ALP檢測等結(jié)果表明,牡蠣殼粉在支架中分布均勻,使支架的力學(xué)性能、蛋白吸附能力、體外礦化能力均得到改善。支架沒有細(xì)胞毒性,能有效促進(jìn)MG63細(xì)胞體外堿性磷酸酶(ALP)的表達(dá)。動(dòng)物試驗(yàn)表明支架無毒、無害。(3)利用3D打印技術(shù)制得的聚己內(nèi)酯/季擕鹽/牡蠣殼粉骨組織工程支架具有三維連通的多孔結(jié)構(gòu),CCK-8檢測、ALP檢測以及抗菌測試等結(jié)果表明用牡蠣殼粉負(fù)載季擕鹽既保持了季擕鹽高效的抗菌性能,又降低了它的細(xì)胞毒性,在不影響牡蠣殼粉生物活性的前提下提高抗菌劑的生物相容性。動(dòng)物試驗(yàn)表明支架對動(dòng)物體無毒、無害。(4)所制得的聚己內(nèi)酯/牡蠣殼粉骨組織工程支架和聚己內(nèi)酯/季擕鹽/牡蠣殼粉抗菌骨組織工程支架具有良好的生物相容性和生物安全性,有望成為一種具有抗菌性能的骨修復(fù)材料。
[Abstract]:According to reports, the number of bone defects caused by natural disasters, traffic accidents, sports injuries and other accidents in China is as high as 300 to 4 million per year. Bone graft is one of the most effective methods to solve the problem of bone defect. A large number of patients need bone repair and bone graft therapy, resulting in a growing demand for bone repair materials at home and abroad. In this study, the biocompatible polycaprolactone (PCLL) and the seasonal salt carrying ATPBN, OSPs, were used as raw materials. The scaffolds for bone tissue engineering of polycaprolactone / oyster shell powder and polycaprolactone / quaternary salt / oyster shell powder scaffolds were prepared by 3D printing technology respectively. The physical and chemical properties and biocompatibility of the scaffolds were evaluated by means of SEM-FTIR DSC, mechanical test, CCK-8 test and ALP test. The conclusions are as follows: (1) after many experiments on the parameters of twin-screw extruder and granulator, the powdered oyster shell powder and polycaprolactone particles were fully mixed and extruded by melt blending extrusion method. Then, polycaprolactone / oyster shell powder and polycaprolactone / quarterly salt / oyster shell powder anti-bacterial 3D printing wire were prepared by special extruder for 3D printing wire. The diameter of 3D printed wire was 1.75 鹵0.2 mm, and the optimum printing temperature was 150 鈩，

本文編號(hào)：1629719