本文選題：骨肉瘤　切入點：黏著斑激酶　出處：《中國腫瘤臨床》2017年10期 　論文類型：期刊論文

【摘要】：目的:探討骨肉瘤組織中黏著斑激酶(focal adhesion kinase,FAK)的表達情況及其與臨床病理特征和預(yù)后的關(guān)系。方法:收集1999年12月至2011年l2月東南大學(xué)附屬中大醫(yī)院和南京軍區(qū)南京總醫(yī)院收治的經(jīng)手術(shù)病理確診的骨肉瘤標本113例,免疫組織化學(xué)檢測病灶中FAK表達量及磷酸化水平,并通過RNA干擾觀察改變FAK的表達與激活水平對骨肉瘤細胞增殖、凋亡、遷移及侵襲活動的影響。結(jié)果:70例標本(61.95%)FAK過表達,其中42例(37.17%)p FAK陽性。FAK表達譜與性別、年齡、AJCCⅡA/ⅡB期、病灶部位、術(shù)式、術(shù)前化療效果等臨床病理參數(shù)均無顯著相關(guān)性。生存分析顯示FAK的過表達及其磷酸化顯著縮短了總生存時間(overall survival,OS)和無轉(zhuǎn)移生存時間(metastasis-free survival,m FS),其與術(shù)前化療效果是預(yù)測骨肉瘤生存時間和轉(zhuǎn)移早晚的兩個獨立預(yù)后因素。細胞學(xué)實驗則顯示FAK的表達和激活可以促進骨肉瘤細胞的增殖、遷移及侵襲活動,并能抑制凋亡。結(jié)論:FAK的過表達及其磷酸化與骨肉瘤惡性程度密切相關(guān),可為生存期及預(yù)后判斷提供一定參考。
[Abstract]:Objective: to investigate the expression of focal adhesion kinase (FAK) in osteosarcoma and its relationship with clinicopathological features and prognosis. Methods: from December 1999 to 2011, the hospital affiliated to Southeast University and the south of Nanjing military region were collected. 113 cases of osteosarcoma confirmed by operation and pathology were treated in Beijing General Hospital. The expression and phosphorylation of FAK were detected by immunohistochemistry, and the effects of FAK expression and activation on the proliferation, apoptosis, migration and invasion of osteosarcoma cells were observed by RNA interference. Among them, 42 cases (37. 17%) showed positive. FAK expression profile and sex, age, AJCC 鈪，

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