發(fā)布時間：2018-03-01 00:03

  本文關(guān)鍵詞： 嗎啡 納洛酮 胃癌 皮下瘤　出處：《臨床麻醉學(xué)雜志》2017年03期 　論文類型：期刊論文

【摘要】：目的觀察納洛酮復(fù)合嗎啡對裸鼠人胃癌皮下瘤生長的影響。方法建立裸鼠人胃癌MGC-803細(xì)胞皮下瘤模型,將50只裸鼠隨機分為五組:對照組(C組)、生理鹽水組(S組)、20mg/kg嗎啡組(M組)、1mg/kg納洛酮組(N組)、1 mg/kg納洛酮+20 mg/kg嗎啡組(NM組),每組10只。成瘤后,C組不作任何處理;S、M、N組裸鼠每天在右下腹分別腹腔注射生理鹽水1.5ml/kg、嗎啡20mg/kg或納洛酮1mg/kg;NM組裸鼠每天在右下腹先腹腔注射納洛酮1mg/kg,30min后再給予嗎啡20mg/kg;連續(xù)注射14d。每2天測量1次腫瘤的長徑和短徑,計算腫瘤相對體積(RTV);用藥結(jié)束后拉頸處死裸鼠,采用透射電鏡觀察腫瘤組織的結(jié)構(gòu)變化,免疫組化、半定量逆轉(zhuǎn)錄-聚合酶鏈反應(yīng)(RT-PCR)和Western blot法檢測腫瘤組織中Cyclin D1、血管內(nèi)皮生長因子(VEGF)、基質(zhì)金屬蛋白酶9(MMP-9)的表達(dá)。結(jié)果 M組裸鼠皮下瘤RTV為(2.21±0.62)%,明顯小于C組的(3.16±0.68)%、S組的(2.98±0.61)%、N組的(3.16±0.35)%和NM組的(2.64±0.37)%(P0.05);NM組裸鼠皮下瘤RTV明顯小于C、S和N組(P0.05)。電鏡下,C、S、N及NM組皮下瘤組織結(jié)構(gòu)基本正常,M組皮下瘤細(xì)胞出現(xiàn)胞漿空泡化、核膜破裂、核染色質(zhì)邊集等。M組裸鼠皮下瘤組織內(nèi)Cyclin D1、VEGF、MMP-9陽性染色腫瘤細(xì)胞、mRNA和蛋白的表達(dá)明顯低于C組(P0.05);NM組裸鼠皮下瘤組織內(nèi)Cyclin D1、VEGF、MMP-9陽性染色腫瘤細(xì)胞、mRNA和蛋白的表達(dá)明顯高于M組(P0.05)。結(jié)論嗎啡可抑制裸鼠人胃癌皮下瘤的生長,納洛酮可拮抗這一作用,其機制可能與其調(diào)節(jié)Cyclin D1、VEGF、MMP-9的表達(dá)有關(guān)。
[Abstract]:Objective to observe the effect of naloxone combined with morphine on the growth of human gastric cancer subcutaneous tumor in nude mice. Fifty nude mice were randomly divided into five groups: control group (n = 10), control group (n = 10), saline group (n = 10), group S (n = 20 mg / kg morphine), group M (n = 1 mg / kg naloxone) and group N (n = 1 mg / kg naloxone 20 mg / kg morphine). Nude mice were injected intraperitoneally with normal saline 1.5ml / kg per day, morphine 20mg / kg or naloxone 1mg / kg NM group respectively. The nude mice were injected intraperitoneally with naloxone 1 mg / kg / kg for 30 minutes and then given morphine 20mg / kg for 14 consecutive days. The long and short diameter of tumor was measured once every 2 days. The relative volume of tumor was calculated and the neck-pulling nude mice were killed after treatment. The structural changes of tumor tissue were observed by transmission electron microscope. Semi-quantitative reverse transcription-polymerase chain reaction (RT-PCR) and Western blot were used to detect the expression of Cyclin D1, vascular endothelial growth factor (VEGF) and matrix metalloproteinase-9 (MMP-9) in tumor tissues. Results the RTV of subcutaneous tumor in group M was 2.21 鹵0.62cm, which was significantly lower than that in group C (3.16 鹵0.68S). The RTV of subcutaneous tumor in group N and group NM were 3.16 鹵0.35% and 2.64 鹵0.37%, respectively. The RTV of subcutaneous tumor in NM group was significantly lower than that in group C (S) and group N (P 0.05). The cytoplasmic vacuolation was observed in subcutaneous tumor cells of group N and group N under electron microscope. Nuclear membrane rupture, The expression of Cyclin D _ (1) VEGF _ (9) -positive staining mRNA and protein in subcutaneous tumor tissue of nude mice in group M was significantly lower than that in group C (P 0.05). The expression of Cyclin D _ (1) VEGFFMP _ 9 positive staining tumor cells in nude mice was significantly higher than that in group C (P 0.05). Conclusion morphine can inhibit the growth of human gastric cancer subcutaneous tumor in nude mice. Naloxone can antagonize this effect, and its mechanism may be related to its regulation of the expression of Cyclin D 1 and VEGFU MMP-9.
【作者單位】： 廣西醫(yī)科大學(xué)第一附屬醫(yī)院麻醉科;
【基金】：國家自然科學(xué)基金(81560500,81160289) 廣西自然科學(xué)基金(2013GXNSFBA019156) 廣西科學(xué)研究與技術(shù)開發(fā)計劃課題(桂科攻1355005-1-6)
【分類號】：R614;R735.2

【相似文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳海蓮;;大劑量新芬太尼與氟烷復(fù)合嗎啡用于新生兒心臟外科手術(shù)麻醉和術(shù)后止痛的效果比較[J];醫(yī)學(xué)信息薈萃;1993年01期

2 陳愛英;馬慧冰;孫仁貴;;布比卡因復(fù)合嗎啡用于剖宮產(chǎn)術(shù)后硬膜外自控鎮(zhèn)痛的臨床觀察[J];現(xiàn)代醫(yī)藥衛(wèi)生;2006年24期

3 曾春梅,蘇永勇;芬太尼復(fù)合嗎啡硬膜外術(shù)后鎮(zhèn)痛效果觀察[J];四川省衛(wèi)生管理干部學(xué)院學(xué)報;2004年03期

4 崔穎;駱龍坤;;地塞米松復(fù)合嗎啡在術(shù)后硬膜外鎮(zhèn)痛的效果觀察[J];中國傷殘醫(yī)學(xué);2008年03期

5 高翔,吳麗娜,張素梅;羅哌卡因復(fù)合嗎啡硬膜外推注用于術(shù)后鎮(zhèn)痛的體會[J];包頭醫(yī)學(xué)院學(xué)報;2002年03期

6 畢燕琳,鄭麗莉,姜敏,艾登斌;膝關(guān)節(jié)腔內(nèi)注射羅哌卡因復(fù)合嗎啡對膝關(guān)節(jié)鏡術(shù)后疼痛的影響[J];中國疼痛醫(yī)學(xué)雜志;2005年05期

7 胡進(jìn)宇;王強;;地塞米松復(fù)合嗎啡行靜脈術(shù)后鎮(zhèn)痛的臨床觀察[J];醫(yī)藥產(chǎn)業(yè)資訊;2006年03期

8 陳銳;唐秀純;黃德衛(wèi);;納洛酮復(fù)合嗎啡術(shù)后硬膜外鎮(zhèn)痛的臨床觀察[J];醫(yī)學(xué)理論與實踐;2009年05期

9 史迎春;戴安盧;張躍軍;;羅哌卡因復(fù)合嗎啡腰麻在剖宮產(chǎn)中的臨床應(yīng)用[J];浙江創(chuàng)傷外科;2006年01期

10 周忠群;;哌替啶復(fù)合雷米芬太尼與哌替啶復(fù)合嗎啡術(shù)后自控靜脈鎮(zhèn)痛效果比較[J];河北醫(yī)藥;2014年08期

相關(guān)會議論文 前2條

1 孫藝丹;徐志新;丁臘枝;;60歲以上少數(shù)民族行泌尿手術(shù)后鎮(zhèn)痛的比較[A];全國第八屆老年護(hù)理學(xué)術(shù)交流暨專題講座會議論文匯編[C];2005年

2 黃禮兵;季淑娟;崔蘇揚;;氟比洛芬酯復(fù)合嗎啡用于高血壓患者圍術(shù)期鎮(zhèn)痛對血漿內(nèi)皮素濃度的影響[A];2008年第七次華東六省一市麻醉學(xué)學(xué)術(shù)會議暨浙江省麻醉學(xué)術(shù)年會論文匯編（下冊）[C];2008年

，

本文編號：1549519