本文關(guān)鍵詞： 七氟醚后處理 心肌缺血再灌注損傷 低氧誘導(dǎo)因子-1 線粒體呼吸功能 呼吸酶活性　出處：《新疆醫(yī)科大學(xué)》2017年碩士論文　論文類型：學(xué)位論文

【摘要】：目的:探討七氟醚后處理是否通過調(diào)控缺氧誘導(dǎo)因子-1α(HIF-1α)來改善線粒體呼吸功能及呼吸酶活性減輕心肌缺血再灌注損傷。方法:88只SD大鼠(SPF級健康雄性)采用Langendorff離體心臟灌流裝置建立大鼠心肌缺血再灌模型,隨機(jī)分為4組(n=22):正常對照組(C)、缺血再灌注組(I/R)、七氟醚后處理組(SpostC)、MSP組【缺血再灌注組(I/R)+HIF-1α阻斷劑(2-Methoxyestradiol,2ME2)+七氟醚后處理組(SpostC):(I/R+2ME2+SpostC)】。C組:持續(xù)灌注Krebs-Henseleit(K-H)液180min;I/R組平衡20min,然后灌注4℃Thomas停跳液,32℃下全心缺血40min后灌注K-H液120min;SpostC組:平衡20min后灌注4℃Thomas停跳液,32℃下全心缺血40min后灌注1.0 MAC七氟醚飽和的K-H液15min,后繼續(xù)灌注正常K-H液105min;MSP組全心缺血40min后灌注含2ME2(2uM)+1.0 MAC七氟醚飽和的K-H液15min后繼續(xù)灌注正常K-H液105min。記錄各組平衡末(T1)及復(fù)灌末(T2)的血流動力學(xué)參數(shù);用漢莎氧電極測定心肌線粒體3態(tài)呼吸(State3)、呼吸控制比(Respiratory Control Ratio,RCR)及煙酰胺腺嘌呤二核苷酸氧化酶(NADHO)、琥珀酸氧化酶(SucO)、細(xì)胞色素C氧化酶(CcO)等酶活性變化;用透射電鏡觀察心肌細(xì)胞超微結(jié)構(gòu)的變化;利用熒光光度法檢測心肌線粒體的活性氧(ROS)產(chǎn)生率;Western blot法檢測HIF-1α蛋白表達(dá)水平。結(jié)果:與缺血再灌注(I/R)組相比,SpostC組的HIF-1α表達(dá)明顯上調(diào),心功能指標(biāo)、線粒體3態(tài)呼吸、呼吸控制比(RCR)、細(xì)胞色素C氧化酶(CcO)、NADH氧化酶(NADHO)、琥珀酸氧化酶(SUCO)的活性、線粒體活性氧產(chǎn)生率明顯優(yōu)于I/R組,但這種優(yōu)勢被HIF-1α特異性抑制劑2ME2完全逆轉(zhuǎn)(P0.05)。結(jié)論:七氟醚后處理通過上調(diào)HIF-1α的表達(dá)改善粒體呼吸功能及呼吸酶的活性減輕大鼠心肌缺血再灌注損傷。
[Abstract]:Objective: to investigate whether sevoflurane post-treatment can improve mitochondrial respiratory function and respiratory enzyme activity by regulating hypoxia inducible factor-1 偽 (HIF-1 偽) to reduce myocardial ischemia-reperfusion injury. Methods: Langendorff dissociation was used in 88 healthy male SD rats. Rat model of myocardial ischemia-reperfusion was established by perfusion device in vivo. The rats were randomly divided into 4 groups: normal control group, ischemia reperfusion group, sevoflurane post-treatment group, SpostCfU group [I / R) HIF-1 偽 blocker 2-Methoxyestradiolol 2ME2ME2ME2ME2)] .C group: continuous perfusion of Ks-rebHeneite K-H) 180 min-IR-IR solution for 20 mins, and then perfusion of 4 groups for 4 min after sevoflurane treatment (SpostCow-r-IR 2ME2SpostC)] .C group: continuous perfusion of Ks-rebHeneititt-K-Har solution for 20 mins. Thomas cardioplegia at 32 鈩，

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