本文關(guān)鍵詞： 氯化鎘 骨肉瘤細胞 細胞凋亡 細胞周期　出處：《實用癌癥雜志》2016年11期 　論文類型：期刊論文

【摘要】：目的通過觀察氯化鎘誘導(dǎo)的骨肉瘤細胞凋亡與細胞特異性周期阻滯及caspase活化的影響,闡明氯化鎘誘導(dǎo)的細胞凋亡效應(yīng)及其分子機制。方法應(yīng)用Annexin V/PI和API等標(biāo)記,流式細胞儀對人骨腫瘤細胞凋亡及周期特異性的檢測,運用RT-PCR的方法檢查Caspase-3、Caspase-9活化情況。結(jié)果處于靜止期的外周血對氯化鎘凋亡誘導(dǎo)不敏感,而G_1期氯化鎘誘導(dǎo)骨肉瘤細胞出現(xiàn)了明顯的細胞凋亡。氯化鎘誘導(dǎo)的細胞凋亡主要是在細胞周期的G_1期發(fā)生。觀察組采用氯化鎘凋亡誘導(dǎo),對照組未做處理,結(jié)果顯示觀察組的Caspase-3、Caspase-9 mRNA的表達值顯著高于對照組。結(jié)論氯化鎘誘導(dǎo)的細胞凋亡與骨肉瘤細胞的細胞周期相關(guān),存在G_1期細胞周期阻滯的周期特異性,且此過程中發(fā)生了caspase活化,參與細胞凋亡。
[Abstract]:Objective to investigate the effects of cadmium chloride on apoptosis, cell specific cycle arrest and caspase activation in osteosarcoma cells, and to elucidate the effect of cadmium chloride on apoptosis and its molecular mechanism. Flow cytometry was used to detect the apoptosis and cycle specificity of human bone tumor cells. The activation of Caspase-3 and Caspase-9 was detected by RT-PCR. Results the peripheral blood in stationary phase was not sensitive to the apoptosis induced by cadmium chloride. The apoptosis of osteosarcoma cells was induced by cadmium chloride at the first phase of Gal 1. The apoptosis of osteosarcoma cells induced by cadmium chloride mainly occurred in the first phase of the cell cycle. The observation group was induced by cadmium chloride apoptosis, but the control group was not treated. The results showed that the expression of Caspase-3 and Caspase-9 mRNA in the observation group was significantly higher than that in the control group. Conclusion the apoptosis induced by cadmium chloride is related to the cell cycle of osteosarcoma cells. Participate in apoptosis.
【作者單位】： 湖北省武漢市普仁醫(yī)院;
【分類號】：R738

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