【摘要】：隨著對絲素蛋白結構和功能的不斷深入研究,絲素的應用發(fā)生了巨大的變化,不再單一地局限于編織,其開發(fā)應用范圍已拓寬到了非紡織領域,如醫(yī)藥化工、保健品、環(huán)境探測等方面。同時,絲素蛋白具有良好的機械性、對生物體無毒且透氣又透氧。因此,在組織工程研究方面表現(xiàn)出不可估量的開發(fā)潛力。 論文首先制備絲素蛋白膜通過溶液澆注而成,應用傅里葉紅外光譜(FTIR)和X-射線衍射(XRD)分析了經(jīng)濃度不同的甲、乙醇-水混合溶劑處理后的絲素蛋白薄膜的結構變化,并以羅丹明B為模型藥物與絲素蛋白建立藥物緩釋系統(tǒng),研究了絲素蛋白膜的結晶結構對藥物釋放動力學的影響情況。實驗發(fā)現(xiàn),在醇體積比濃度φMeOH/%=50～90的范圍內,絲素蛋白材料中以p-折疊為主的silk Ⅱ結晶含量隨著混合溶劑中醇濃度的增加而先增加后下降。甲醇-水混合溶劑中,在φMeOH/%=80附近出現(xiàn)最大值。而在乙醇-水混合溶劑中,在70-80%附近出現(xiàn)最大值。應用Peppas方程分析了羅丹明B從絲素蛋白膜的釋放動力學,結果表明,羅丹明B從絲素蛋白膜的釋放屬于Fickian擴散機理,其擴散指數(shù)n隨著絲素蛋白膜中p-折疊含量的增加而增加。silk Ⅱ結晶是絲素蛋白材料藥物釋放的天然調節(jié)器。 在此基礎上選用不同比例的丙三醇與絲素蛋白共混成膜,應用傅里葉紅外光譜(FTIR)和X-射線衍射(XRD)研究了絲素蛋白-丙三醇共混膜的結晶結構的變化,并以羅丹明B為模型藥物與絲素蛋白建立藥物緩釋系統(tǒng),研究了絲素蛋白膜的結晶結構對藥物釋放動力學的影響情況。結果顯示,在未經(jīng)甲醇-水混合溶劑處理的絲素蛋白-丙三醇共混膜中,隨著丙三醇含量的提高,絲素蛋白材料中以p-折疊為主的silk Ⅱ結晶結構含量逐漸增大,在丙三醇含量為30%時出現(xiàn)最大值。羅丹明B從共混藥物膜的釋放屬于Fickian擴散機理,其擴散指數(shù)n隨著絲素蛋白膜中β-折疊含量的增加而增加,且藥物釋放百分數(shù)增大,丙三醇的存在可以改善絲素蛋白為載體的藥物釋放效果。
[Abstract]:With the in-depth study of the structure and function of silk fibroin, the application of silk fibroin has changed greatly, and it is no longer confined to weaving, and its development and application has been extended to non-textile fields, such as medicine, chemical industry, health products, etc. Environmental detection etc. At the same time, silk fibroin has good mechanical properties, non-toxic to organism, breathable and oxygen permeable. Therefore, there is inestimable development potential in tissue engineering research. Firstly, silk fibroin films were prepared by solution pouring. The structural changes of silk fibroin films treated with different concentrations of methyl-ethanol-water solution were analyzed by Fourier transform infrared spectroscopy (FTIR) and X-ray diffraction (XRD). The drug release system was established with Rhodamine B as model drug and silk fibroin. The effect of crystalline structure of silk fibroin membrane on drug release kinetics was studied. It was found that in the range of alcohol volume ratio 蠁 MeOH/%=50~90, the content of silk 鈪，

本文編號：2367265