表面改性納米羥基磷灰石及與PLLA納米復合纖維的制備與表征
發(fā)布時間：2018-11-23 21:05
【摘要】：隨著化學、材料科學、醫(yī)學和生物學的迅速發(fā)展,使骨科修復材料也隨之產生了巨大變革,從金屬合金材料、生物惰性陶瓷材料發(fā)展到當今的生物活性材料,現在以生物活性材料為基底構建骨組織工程支架材料,然后經過組織培養(yǎng)發(fā)育成人體骨組織,最終實現自體組織對植入體的完全替代,將極大地減輕病人痛苦,降低醫(yī)療成本以及減少術后并發(fā)癥。羥基磷灰石是一種近年來研究最為深入的生物活性材料,它是哺乳動物骨骼和牙齒的主要無機物組分,具有骨誘導性和骨傳導性。但是羥基磷灰石的脆性大、韌性差,限制了其在承重部位骨的修復的應用。通過與聚合物復合,制備羥基磷灰石/聚合物復合材料,能夠有效地結合羥基磷灰石的骨傳導性能和聚合物的韌性等優(yōu)點,但是羥基磷灰石與聚合物基體之間的界面相容性比較差,無機粒子在聚合物基體中易團聚限制了其復合材料力學性能的提高。本論文提出了對羥基磷灰石的表面進行改性來提高羥基磷灰石在聚合物基體中的分散作用,增強有機相與無機相之間的界面作用力,此外,還研究了改性羥基磷灰石與聚乳酸(PLLA)復合制備了復合納米纖維材料,對復合納米纖維的力學性能、可浸濕性、形貌進行了初步的研究： 第2章,利用在制備羥基磷灰石的過程中加入丙炔酸,獲得表面含有活性炔基的羥基磷灰石(AHA),然后再與疊氮基改性的殼聚糖(CS-N3)發(fā)生click反應,從而獲得了表面接枝殼聚糖的羥基磷灰石(HA-CS)。通過紅外光譜,X射線光電子能譜對反應產物進行了鑒定。熱重分析表明,殼聚糖的接枝量為8.9%。廣角X射線衍射(XRD)結果表明,這種改性方法只在納米粒子表面進行,沒有破壞納米粒子的晶體結構。改性后的羥基磷灰石的親水性能得到了提高,能夠在水中均勻分散,其生物相容性明顯優(yōu)于納米羥基磷灰石。 第3章,利用γ-氨基丙基三乙氧基硅烷對納米羥基磷灰石進行表面處理,獲得表面具有活性氨基的納米羥基磷灰石(HA-NH2),再與2-溴異丁酰溴反應得到表面含有ATRP引發(fā)劑的改性納米羥基磷灰石(HA-Br),再引發(fā)糖烯單體2-(四乙酰基-p-D-吡喃葡萄糖)甲基丙烯酸乙酯聚合,從而在納米羥基磷灰石的表面接枝了含糖聚合物的改性的納米羥基磷灰石。利用靜電紡絲法制備了含糖聚合物改性的羥基磷灰石/(PLLA)復合材料。通過紅外光譜、核磁共振氫譜、熱失重、XRD、SEM等分析手段對產物進行了表征。
[Abstract]:With the rapid development of chemistry, material science, medicine and biology, the orthopaedic repair materials have been transformed, from metal alloy materials, bio-inert ceramic materials to bioactive materials. Now using bioactive material as the substrate to construct bone tissue engineering scaffold material, and then develop into human bone tissue through tissue culture, and finally realize the complete replacement of autogenous tissue to implants, which will greatly alleviate the suffering of patients. Reduce medical costs and postoperative complications. Hydroxyapatite is a bioactive material which has been studied in recent years. Hydroxyapatite is the main inorganic component of mammalian bone and teeth. It has bone inductivity and bone conductivity. However, hydroxyapatite has a large brittleness and poor toughness, which limits the application of hydroxyapatite in bone repair. Hydroxyapatite / polymer composites were prepared by blending with polymers, which could effectively combine the bone conductivity of hydroxyapatite and the toughness of polymers. However, the interfacial compatibility between hydroxyapatite and polymer matrix is poor, and the easy agglomeration of inorganic particles in the polymer matrix limits the improvement of the mechanical properties of the composites. In this paper, the surface modification of hydroxyapatite is proposed to improve the dispersion of hydroxyapatite in polymer matrix and to enhance the interfacial force between organic phase and inorganic phase. The modified hydroxyapatite and poly (lactic acid) (PLLA) composite nanofibers were prepared. The mechanical properties, wettability and morphology of the composite nanofibers were studied. Hydroxyapatite (AHA), was obtained by adding propionic acid in the preparation of hydroxyapatite and then reacted with azido modified chitosan (CS-N3) by click. The surface grafted chitosan hydroxyapatite (HA-CS) was obtained. The reaction products were identified by IR and X-ray photoelectron spectroscopy. Thermogravimetric analysis showed that the graft amount of chitosan was 8.9. The results of wide-angle X-ray diffraction (XRD) show that the modified method only takes place on the surface of nanoparticles and does not destroy the crystal structure of nanoparticles. The hydrophilicity of the modified hydroxyapatite was improved, and the hydrophilic property of the modified hydroxyapatite was improved, and the biocompatibility of the modified hydroxyapatite was obviously better than that of nano-hydroxyapatite. In chapter 3, nano-hydroxyapatite (HA-NH2) with active amino group was obtained by surface treatment of nano-hydroxyapatite with 緯 -aminopropyl triethoxy silane. The modified nano-hydroxyapatite (HA-Br) containing ATRP initiator was obtained by reaction with 2-bromoisobutylol bromide, and then the glycosene monomer 2- (tetraacetyl-p-D-glucopyranose) ethyl methacrylate was polymerized. As a result, the surface of nano-hydroxyapatite was grafted onto sucrose polymer modified nano-hydroxyapatite. Hydroxyapatite / (PLLA) composites modified by sucrose polymer were prepared by electrostatic spinning. The products were characterized by IR, NMR, TG and XRD,SEM.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08
本文編號：2352700
[Abstract]:With the rapid development of chemistry, material science, medicine and biology, the orthopaedic repair materials have been transformed, from metal alloy materials, bio-inert ceramic materials to bioactive materials. Now using bioactive material as the substrate to construct bone tissue engineering scaffold material, and then develop into human bone tissue through tissue culture, and finally realize the complete replacement of autogenous tissue to implants, which will greatly alleviate the suffering of patients. Reduce medical costs and postoperative complications. Hydroxyapatite is a bioactive material which has been studied in recent years. Hydroxyapatite is the main inorganic component of mammalian bone and teeth. It has bone inductivity and bone conductivity. However, hydroxyapatite has a large brittleness and poor toughness, which limits the application of hydroxyapatite in bone repair. Hydroxyapatite / polymer composites were prepared by blending with polymers, which could effectively combine the bone conductivity of hydroxyapatite and the toughness of polymers. However, the interfacial compatibility between hydroxyapatite and polymer matrix is poor, and the easy agglomeration of inorganic particles in the polymer matrix limits the improvement of the mechanical properties of the composites. In this paper, the surface modification of hydroxyapatite is proposed to improve the dispersion of hydroxyapatite in polymer matrix and to enhance the interfacial force between organic phase and inorganic phase. The modified hydroxyapatite and poly (lactic acid) (PLLA) composite nanofibers were prepared. The mechanical properties, wettability and morphology of the composite nanofibers were studied. Hydroxyapatite (AHA), was obtained by adding propionic acid in the preparation of hydroxyapatite and then reacted with azido modified chitosan (CS-N3) by click. The surface grafted chitosan hydroxyapatite (HA-CS) was obtained. The reaction products were identified by IR and X-ray photoelectron spectroscopy. Thermogravimetric analysis showed that the graft amount of chitosan was 8.9. The results of wide-angle X-ray diffraction (XRD) show that the modified method only takes place on the surface of nanoparticles and does not destroy the crystal structure of nanoparticles. The hydrophilicity of the modified hydroxyapatite was improved, and the hydrophilic property of the modified hydroxyapatite was improved, and the biocompatibility of the modified hydroxyapatite was obviously better than that of nano-hydroxyapatite. In chapter 3, nano-hydroxyapatite (HA-NH2) with active amino group was obtained by surface treatment of nano-hydroxyapatite with 緯 -aminopropyl triethoxy silane. The modified nano-hydroxyapatite (HA-Br) containing ATRP initiator was obtained by reaction with 2-bromoisobutylol bromide, and then the glycosene monomer 2- (tetraacetyl-p-D-glucopyranose) ethyl methacrylate was polymerized. As a result, the surface of nano-hydroxyapatite was grafted onto sucrose polymer modified nano-hydroxyapatite. Hydroxyapatite / (PLLA) composites modified by sucrose polymer were prepared by electrostatic spinning. The products were characterized by IR, NMR, TG and XRD,SEM.
【學位授予單位】：南昌大學
【學位級別】：碩士
【學位授予年份】：2012
【分類號】：R318.08
【參考文獻】
相關期刊論文 前1條
1 劉永磊;李鴻;呂國玉;南景天;羅小滿;嚴永剛;;n-HA/多元氨基酸共聚物復合材料的制備和界面研究[J];功能材料;2010年04期
，本文編號：2352700
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