【摘要】：文中以微孔淀粉為主體,研究一種新型復(fù)合止血材料的制備及生物學評價。此復(fù)合材料由一定比例的α-淀粉酶與糖化酶復(fù)合后處理淀粉以形成微孔結(jié)構(gòu),然后復(fù)合類人膠原蛋白,再以戊二醛為交聯(lián)劑進行交聯(lián);用SD大鼠作肝臟出血模型進行止血效果對比試驗。通過正交試驗確定微孔淀粉最優(yōu)制備工藝條件如下:α-淀粉酶:糖化酶=1∶3,T/℃=50,t/h=20,pH=4.8;用0.2%(V/V)戊二醛乙醇溶液交聯(lián)的類人膠原蛋白-微孔淀粉止血材料,在相同的止血時間下,該復(fù)合材料平均吸水率為3 580%,對照組(分別是微孔淀粉和類人膠原蛋白海綿)的平均吸水率為3 160%和3 280%。而在SD大鼠肝臟實驗中,復(fù)合材料t/s=94±13,而外購的止血劑t/s=121±15。類人膠原蛋白-微孔淀粉復(fù)合材料止血的止血原理從3個方面展開:①微孔淀粉吸收水分并將血液中的有形物質(zhì)聚集到表面;②加速內(nèi)源性凝血因子的激活使其形成血栓;③在類人膠原蛋白的牽引下,淀粉顆粒聚成團大幅度增加了比表面積,使止血效果更優(yōu)秀;對照組止血機理只包含了該復(fù)合材料的前兩個方面。結(jié)果顯示,復(fù)合材料的止血效果要優(yōu)于普通微孔淀粉制成的止血劑,證明該復(fù)合止血材料具有良好的止血性能。
[Abstract]:In this paper, the preparation and biological evaluation of a new compound hemostatic material with microporous starch as the main body were studied. The composite was treated with a certain proportion of 偽 -amylase and Glucoamylase to form micropore structure, and then compound human collagen-like protein, then crosslinked with glutaraldehyde as crosslinking agent. The hemostatic effect of SD rats was compared with the model of hepatic hemorrhage. The optimum preparation conditions of microporous starch were determined by orthogonal test as follows: 偽 -amylase: Glucoamylase = 1: 3T / 鈩，

本文編號：2352547