【摘要】：NiTi合金由于其獨(dú)特的形狀記憶效應(yīng)、超彈性、高耐腐蝕性和良好的生物相容性等性能，已在生物醫(yī)學(xué)領(lǐng)域得到廣泛應(yīng)用。然而，NiTi合金植入材料表面容易誘導(dǎo)血栓形成，是其臨床應(yīng)用中面臨的主要挑戰(zhàn)之一。此外，NiTi合金植入后在組織周圍釋放Ni2+可能會(huì)產(chǎn)生過敏反應(yīng)和毒副作用。因此，通過NiTi合金基材的表面工程促進(jìn)血管內(nèi)皮細(xì)胞的功能（血管內(nèi)皮化）同時(shí)減少Ni2+釋放成為NiTi合金植入材料的研究重點(diǎn)。 研究表明：減少Ni2+釋放量的一種有效方法是在NiTi合金表面形成一層保護(hù)涂層，，且該涂層可提高NiTi合金植入物的生物相容性，進(jìn)而調(diào)控內(nèi)皮細(xì)胞的生理功能。眾所周知，細(xì)胞微環(huán)境在調(diào)節(jié)細(xì)胞行為方面發(fā)揮著重要作用，細(xì)胞行為（如黏附、遷移、增殖、分化等）都受到材料表面的化學(xué)成分和形貌的影響。在材料表面固定細(xì)胞外基質(zhì)成分或生長(zhǎng)因子可以改變材料表面的化學(xué)組成，從而促進(jìn)細(xì)胞與生物材料相互作用，增強(qiáng)細(xì)胞的功能。內(nèi)皮細(xì)胞生長(zhǎng)因子（VEGF）可以促進(jìn)血管內(nèi)皮細(xì)胞的遷移、增殖并形成管狀結(jié)構(gòu)，從而促進(jìn)血管的新生。 本文首先采用磁控濺射和水熱氧化方法在NiTi合金表面構(gòu)建納米氧化鋁涂層，然后以多巴胺為中間界層在其表面固定VEGF。利用X射線衍射（XRD）、掃描電鏡（SEM）、原子力掃描電鏡（AFM）、電感耦合等離子體質(zhì)譜儀（ICP-MS）和X-射線光電子能譜儀（XPS）對(duì)相關(guān)材料進(jìn)行了表征。材料表面為無定型的多孔的針狀納米結(jié)構(gòu)氧化鋁涂層，其均方根粗糙度為22±4nm，厚度大約為200nm。該涂層顯著降低了材料表面的Ni2+釋放量。同時(shí)，我們對(duì)人臍靜脈內(nèi)皮細(xì)胞在材料表面的細(xì)胞增殖、細(xì)胞形貌、細(xì)胞鋪展以及細(xì)胞分泌的前列環(huán)素I2（PGI2）和一氧化氮（NO）進(jìn)行了檢測(cè)。結(jié)果表明：接種在VEGF修飾后的NiTi合金表面的人臍靜脈內(nèi)皮細(xì)胞與納米涂層的NiTi合金以及未修飾的NiTi合金相比，具有更高的活力(p 0.05或p 0.01)，并且內(nèi)皮細(xì)胞在VEGF修飾后NiTi合金表面的數(shù)量更多，鋪展面積更大。同時(shí)，內(nèi)皮細(xì)胞在VEGF修飾后NiTi合金表面PGI2以及NO的分泌量與未修飾的NiTi合金相比明顯增加，均具有顯著性差異性（p 0.05）。本研究為NiTi合金在生物醫(yī)學(xué)領(lǐng)域的廣泛應(yīng)用積累了有益的實(shí)驗(yàn)數(shù)據(jù)。
[Abstract]:NiTi alloys have been widely used in biomedical fields due to their unique shape memory effect, super elasticity, high corrosion resistance and good biocompatibility. However, the surface of Niti alloy implant is easy to induce thrombosis, which is one of the main challenges in clinical application. In addition, the release of Ni2 around the tissue may result in allergic reactions and toxic side effects after implantation of Niti alloy. Therefore, the surface engineering of NiTi alloy substrate can promote the function of vascular endothelial cells (vascular endothelialization) and reduce the release of Ni2. The results showed that an effective way to reduce the release of Ni2 was to form a protective coating on the surface of NiTi alloy, which could improve the biocompatibility of NiTi alloy implants and regulate the physiological function of endothelial cells. It is well known that cell microenvironment plays an important role in regulating cell behavior. Cell behavior (such as adhesion migration proliferation differentiation and so on) is affected by the chemical composition and morphology of the material surface. Immobilization of extracellular matrix or growth factor on the surface of the material can change the chemical composition of the material surface, thus promote the interaction between cells and biomaterials and enhance the function of cells. Endothelial growth factor (VEGF) can promote vascular endothelial cell migration, proliferation and formation of tubular structure, thereby promoting angiogenesis. In this paper, nanocrystalline Al _ 2O _ 3 coating was prepared on NiTi alloy by magnetron sputtering and hydrothermal oxidation. Then VEGF. was fixed on the surface of NiTi alloy with dopamine as the intermediate layer. The related materials were characterized by X-ray diffraction (XRD), scanning electron microscope (XRD), (SEM), atomic force scanning electron microscope (AFM), inductively coupled plasma mass spectrometer (ICP-MS) and X-ray photoelectron spectroscopy (XPS). The surface of the material is an amorphous porous needle-like nano-structure alumina coating with a root-mean-square roughness of 22 鹵4 nm and a thickness of about 200 nm. The Ni2 emission on the surface of the material was significantly reduced by the coating. At the same time, the cell proliferation, cell morphology, cell spreading, prostacyclin I 2 (PGI2) and nitric oxide (NO) (no (NO) of human umbilical vein endothelial cells on the surface of materials were detected. The results showed that the human umbilical vein endothelial cells inoculated on the surface of VEGF modified NiTi alloy were compared with nano-coated NiTi alloy and unmodified NiTi alloy. It has higher activity (p0.05 or p0.01), and the number of endothelial cells on the surface of VEGF modified NiTi alloy is more and the spreading area is larger. At the same time, the secretion of PGI2 and NO on the surface of VEGF modified NiTi alloy was significantly higher than that of unmodified NiTi alloy (p0.05). This study has accumulated useful experimental data for the wide application of NiTi alloy in biomedical field.
【學(xué)位授予單位】：重慶大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

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