【摘要】：針對(duì)當(dāng)前水凝膠構(gòu)建實(shí)質(zhì)性功能器官存在制備血管網(wǎng)絡(luò)困難的問(wèn)題,采用了轉(zhuǎn)谷氨酰胺酶(mTG酶)交聯(lián)明膠構(gòu)建明膠水凝膠圓形仿生微流道的方法。研究了明膠水凝膠的力學(xué)性能、成形性能及生物性能與明膠濃度的關(guān)系,提出了明膠水凝膠構(gòu)建微流道結(jié)構(gòu)的工藝條件,評(píng)價(jià)了微流道構(gòu)建工藝的成型精度、力學(xué)性能和內(nèi)皮化功能。結(jié)果表明:隨著水凝膠濃度的提高,水凝膠的力學(xué)性能成線(xiàn)性增加,微結(jié)構(gòu)的成型性能也逐步改善,但是濃度過(guò)高生物性能反而降低,明膠質(zhì)量濃度為0.1g/mL的水凝膠綜合性能相對(duì)較好,可用于構(gòu)建圓形仿生微流道;采用的微流道成型工藝保持了原有水凝膠的力學(xué)性能,但是存在一定的成型誤差,細(xì)胞在微流道內(nèi)分布均勻,實(shí)現(xiàn)了微流道的內(nèi)皮化。通過(guò)研究不同濃度的酶交聯(lián)明膠水凝膠,制備的仿生微流道有望用于實(shí)質(zhì)性功能器官的仿生再建。
[Abstract]:In view of the difficulty of preparing vascular network in the construction of solid functional organs by hydrogels, the circular bionic microchannels of gelatin hydrogels were constructed by transglutaminase (mTG) cross-linking gelatin. The relationship between the mechanical properties, formability and biological properties of gelatin hydrogels and the concentration of gelatin was studied. The technological conditions for the construction of microchannel structures by gelatin hydrogels were put forward, and the forming accuracy of the microchannel construction process was evaluated. Mechanical properties and endothelial function. The results show that with the increase of hydrogel concentration, the mechanical properties of hydrogels increase linearly, and the molding properties of microstructures are improved gradually, but the biological properties of hydrogels with high concentration decrease. The hydrogels with gelatin concentration of 0.1g/mL have relatively good comprehensive properties, and can be used to construct circular bionic microchannels. The mechanical properties of the original hydrogels are maintained by the microchannel molding process, but there are some forming errors. The cells were evenly distributed in the microchannel and the endothelialization of the microchannel was realized. By studying gelatin hydrogels with different concentrations of enzyme crosslinking, the biomimetic microchannels are expected to be used in the bionic reconstruction of solid functional organs.
【作者單位】： 西安交通大學(xué)機(jī)械制造系統(tǒng)工程國(guó)家重點(diǎn)實(shí)驗(yàn)室;
【基金】：國(guó)家自然科學(xué)基金資助項(xiàng)目(51675416) 陜西省統(tǒng)籌項(xiàng)目(2015KTTSGY-04)
【分類(lèi)號(hào)】：R318.08

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