用于引導(dǎo)牙周組織再生殼聚糖基隔離膜的制備及其表征
發(fā)布時(shí)間：2018-08-01 17:02
【摘要】：牙周炎是一種導(dǎo)致牙周組織逐漸損壞近而引起成人牙齒缺失的炎癥疾病。一種治療牙周病的理想結(jié)果就是在采用牙周再生技術(shù)進(jìn)行牙周病治療引起感染之前使牙周組織長(zhǎng)回原位,以恢復(fù)其原始結(jié)構(gòu)和功能。骨移植和引導(dǎo)組織再生技術(shù)是近年來(lái)治療牙周組織缺損的重要手段。本論文采用聚乙烯醇(PVA)對(duì)殼聚糖(CS)膜進(jìn)行改性以提高其實(shí)際應(yīng)用價(jià)值；將冰硼散(BPS)載入到CS膜中避免引導(dǎo)牙周組織再生過(guò)程中引起細(xì)菌感染；將骨移植法和引導(dǎo)組織再生技術(shù)結(jié)合起來(lái)促進(jìn)CS膜的生物活性。 (1)溶液澆注法制備CS/PVA復(fù)合膜 采用溶液澆鑄法制備了CS/PVA復(fù)合膜。綜合研究了復(fù)合膜的化學(xué)性質(zhì)、物理性能、熱穩(wěn)定性能、表面潤(rùn)濕性能、力學(xué)性能、降解性能以及生物相容性。研究發(fā)現(xiàn)CS與PVA通過(guò)形成分子間和分子內(nèi)氫鍵具有強(qiáng)的相互作用。當(dāng)CS含量占主要成分時(shí),CS與PVA的相容性良好；當(dāng)PVA含量近一步增加時(shí)出現(xiàn)一定程度的相分離。添加PVA可以大大提高CS膜的韌性和改善表面潤(rùn)濕性能,調(diào)控CS膜的降解性能以及降解過(guò)程中表面形貌。CS/PVA復(fù)合膜的生物相容性良好,在引導(dǎo)組織再生領(lǐng)域具有潛在的應(yīng)用價(jià)值。 (2)凝膠法制備CS/BPS復(fù)合膜 采用凝膠法制備出CS/BPS復(fù)合膜。該方法避免了BPS在CS膜后處理過(guò)程中受到酸堿的影響,保持了藥物活性。添加BPS使得CS分子內(nèi)和分子間氫鍵密度降低,增加了自由氨基和羥基含量。當(dāng)CS與BPS質(zhì)量比較高時(shí),CS與BPS混合性良好,無(wú)明顯相分離；當(dāng)其質(zhì)量比較低時(shí),混合性變差,出現(xiàn)相分離。CS/BPS復(fù)合膜表面潤(rùn)濕性能良好,力學(xué)性能能夠滿足引導(dǎo)牙周組織再生應(yīng)用。CS/BPS復(fù)合膜能夠促進(jìn)細(xì)胞在其表面的鋪展、生長(zhǎng)和增殖。 (3)電化學(xué)法仿生礦化CS凝膠制備有機(jī)／無(wú)機(jī)雜化材料 以CS凝膠為模板采用電化學(xué)法制備出僅一邊具有生物活性礦物的不對(duì)稱結(jié)構(gòu)的殼聚糖／羥基磷灰石／碳酸鈣雜化材料。CS氨基與鈣離子絡(luò)合作用較強(qiáng),pH值對(duì)礦物形成具有重大影響。陽(yáng)極一側(cè)無(wú)礦物產(chǎn)生,陰極一側(cè)CS凝膠表面產(chǎn)生直徑5-6μm礦物粒子,并且單一粒子均有幾納米大小的顆粒組成。與將材料浸入到模擬體液進(jìn)行生物礦化的方法相比,該方法大大降低了礦化時(shí)間。隨著礦化時(shí)間增加,Ca/P比逐漸降低,但高于1.65(羥基磷灰石的Ca/P比)。高Ca/P比能夠確保有機(jī)/無(wú)機(jī)雜化材料具有較高含量的Ca和足夠多的P來(lái)維持較高水平的骨礦物密度。
[Abstract]:Periodontitis is an inflammatory disease that causes periodontal tissue damage and tooth loss in adults. An ideal outcome for the treatment of periodontal disease is to restore the periodontal tissue to its original structure and function before periodontal regeneration is used to treat the infection. Bone transplantation and guided tissue regeneration are important methods for the treatment of periodontal tissue defects in recent years. In this paper, polyvinyl alcohol (PVA) was used to modify chitosan (CS) membrane to improve its practical application value, and the (BPS) was loaded into CS membrane to avoid bacterial infection during periodontal tissue regeneration. Bone graft and guided tissue regeneration were combined to promote the biological activity of CS membrane. (1) CS/PVA composite membrane was prepared by solution casting method and CS/PVA composite membrane was prepared by solution casting method. The chemical properties, physical properties, thermal stability, surface wettability, mechanical properties, degradation properties and biocompatibility of the composite films were studied. It is found that CS and PVA interact strongly by forming intermolecular and intramolecular hydrogen bonds. The compatibility between CS and PVA was good when CS content was the main component, and the phase separation appeared when the content of PVA increased one step later. The addition of PVA can greatly improve the toughness and wettability of CS film, and regulate the degradation performance of CS film and the biocompatibility of CS- / PVA composite film during the degradation process. It has potential application value in guiding tissue regeneration. (2) CS/BPS composite membrane was prepared by gel method and CS/BPS composite membrane was prepared by gel method. This method avoids the influence of acid and base on BPS during CS membrane aftertreatment, and maintains the drug activity. The addition of BPS reduced the intramolecular and intermolecular hydrogen bonding densities of CS and increased the free amino and hydroxyl contents. When the mass ratio of CS to BPS is high, the mixture of CS and BPS is good, and when the mass is low, the mixing property becomes worse, and the surface wettability of CS- / BPS composite membrane is good. Mechanical properties can be used to guide periodontal tissue regeneration. CS- / BPS composite membrane can promote cell spreading on its surface. (3) preparation of organic / inorganic hybrid materials by electrochemical biomimetic mineralized CS gel using CS gel as template to prepare asymmetric junctions with only one side of biologically active minerals prepared by electrochemical method Chitosan / hydroxyapatite / calcium carbonate hybrid material. CS amino complex with calcium ions has a strong interaction. The pH value has a significant impact on mineral formation. No minerals were produced on the anode side, and the surface of CS gel on the cathode side produced 5-6 渭 m mineral particles in diameter, and the single particles were composed of several nanometer-sized particles. Compared with the method of imitating body fluid for biomineralization, this method greatly reduces the time of mineralization. The Ca / P ratio decreased gradually with the increase of mineralization time, but was higher than 1.65 (Ca/P ratio of hydroxyapatite). High Ca/P ratio can ensure that organic / inorganic hybrid materials have high Ca content and sufficient P to maintain high bone mineral density.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：TB383.2;R783.1
本文編號(hào)：2158242
[Abstract]:Periodontitis is an inflammatory disease that causes periodontal tissue damage and tooth loss in adults. An ideal outcome for the treatment of periodontal disease is to restore the periodontal tissue to its original structure and function before periodontal regeneration is used to treat the infection. Bone transplantation and guided tissue regeneration are important methods for the treatment of periodontal tissue defects in recent years. In this paper, polyvinyl alcohol (PVA) was used to modify chitosan (CS) membrane to improve its practical application value, and the (BPS) was loaded into CS membrane to avoid bacterial infection during periodontal tissue regeneration. Bone graft and guided tissue regeneration were combined to promote the biological activity of CS membrane. (1) CS/PVA composite membrane was prepared by solution casting method and CS/PVA composite membrane was prepared by solution casting method. The chemical properties, physical properties, thermal stability, surface wettability, mechanical properties, degradation properties and biocompatibility of the composite films were studied. It is found that CS and PVA interact strongly by forming intermolecular and intramolecular hydrogen bonds. The compatibility between CS and PVA was good when CS content was the main component, and the phase separation appeared when the content of PVA increased one step later. The addition of PVA can greatly improve the toughness and wettability of CS film, and regulate the degradation performance of CS film and the biocompatibility of CS- / PVA composite film during the degradation process. It has potential application value in guiding tissue regeneration. (2) CS/BPS composite membrane was prepared by gel method and CS/BPS composite membrane was prepared by gel method. This method avoids the influence of acid and base on BPS during CS membrane aftertreatment, and maintains the drug activity. The addition of BPS reduced the intramolecular and intermolecular hydrogen bonding densities of CS and increased the free amino and hydroxyl contents. When the mass ratio of CS to BPS is high, the mixture of CS and BPS is good, and when the mass is low, the mixing property becomes worse, and the surface wettability of CS- / BPS composite membrane is good. Mechanical properties can be used to guide periodontal tissue regeneration. CS- / BPS composite membrane can promote cell spreading on its surface. (3) preparation of organic / inorganic hybrid materials by electrochemical biomimetic mineralized CS gel using CS gel as template to prepare asymmetric junctions with only one side of biologically active minerals prepared by electrochemical method Chitosan / hydroxyapatite / calcium carbonate hybrid material. CS amino complex with calcium ions has a strong interaction. The pH value has a significant impact on mineral formation. No minerals were produced on the anode side, and the surface of CS gel on the cathode side produced 5-6 渭 m mineral particles in diameter, and the single particles were composed of several nanometer-sized particles. Compared with the method of imitating body fluid for biomineralization, this method greatly reduces the time of mineralization. The Ca / P ratio decreased gradually with the increase of mineralization time, but was higher than 1.65 (Ca/P ratio of hydroxyapatite). High Ca/P ratio can ensure that organic / inorganic hybrid materials have high Ca content and sufficient P to maintain high bone mineral density.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：TB383.2;R783.1
【參考文獻(xiàn)】
相關(guān)碩士學(xué)位論文 前1條
1 常秀梅;組織工程方法構(gòu)建牙周組織的實(shí)驗(yàn)研究[D];第一軍醫(yī)大學(xué);2005年
，本文編號(hào)：2158242
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