本文選題：皮膚組織工程 + 三維打印�。� 參考：《浙江大學(xué)》2017年碩士論文

【摘要】：皮膚組織工程的基本原理是將體外擴(kuò)增培養(yǎng)的皮膚種子細(xì)胞和具有優(yōu)良生物相容性的材料相結(jié)合,建立細(xì)胞與生物材料之間的三維空間復(fù)合體,其基本要素包括種子細(xì)胞、支架材料和組織構(gòu)建。采用生物三維打印技術(shù)能夠精確定位多種類型的基質(zhì)材料和細(xì)胞,具有孔隙結(jié)構(gòu)可控、寬尺寸范圍及高生產(chǎn)能力等優(yōu)點(diǎn),在組織工程皮膚的構(gòu)建中具有極大的潛力。本學(xué)位論文采用自主研發(fā)的生物三維打印系統(tǒng)開展水凝膠支架與皮膚細(xì)胞的打印實驗研究。在分析水凝材料基本特點(diǎn)的基礎(chǔ)上,對材料擠出過程進(jìn)行了計算與仿真分析。同時采用三維打印的方式進(jìn)行了皮膚組織工程支架與細(xì)胞打印的實驗研究,結(jié)果表明本系統(tǒng)具有良好的支架成型能力,構(gòu)建的組織支架能夠為細(xì)胞提供適宜的三維生長環(huán)境。論文主要展開的工作內(nèi)容如下:一、面向皮膚組織工程的生物三維打印系統(tǒng)設(shè)計與研發(fā)。結(jié)合現(xiàn)有生物三維打印技術(shù)的基本原理與皮膚組織工程的具體要求,提出了基于氣動擠出原理的柔性打印方案。系統(tǒng)采用獨(dú)立雙噴頭結(jié)構(gòu),分為運(yùn)動系統(tǒng)、供·系統(tǒng)、溫控系統(tǒng)、交聯(lián)系統(tǒng)和環(huán)境控制等五部分,可用于多種生物材料及細(xì)胞打印研究。二、水凝膠材料擠出過程分析。從冪律流體的本構(gòu)方程建立了水凝膠材料從噴頭擠出后的纖維寬度、支架層高及孔隙率的數(shù)學(xué)模型,并采用流體仿真的手段對材料擠出過程中的流速、壁面剪切應(yīng)力、擠出脹大現(xiàn)象等進(jìn)行了分析,為打印參數(shù)的控制優(yōu)化提供了理論支持與指導(dǎo)。三、皮膚組織工程支架設(shè)計與打印。根據(jù)人體皮膚的結(jié)構(gòu)特點(diǎn)設(shè)計了皮膚支架的三維模型,從多邊形輪廓的特征識別、掃描求交、區(qū)域分組、路徑填充等方面進(jìn)行了打印算法的研究,并采用ACSPL+語言進(jìn)行支架打印程序設(shè)計,最終打印出不同結(jié)構(gòu)參數(shù)的三維支架結(jié)構(gòu)。四、種子細(xì)胞打印與三維培養(yǎng)。選取皮膚成纖維細(xì)胞作為種子細(xì)胞進(jìn)行細(xì)胞打印與三維培養(yǎng)實驗研究,采用熒光染料法對打印后及培養(yǎng)中的細(xì)胞進(jìn)行了活性分析,觀察記錄成纖維細(xì)胞在水凝膠三維支架中的生長、增殖及遷移情況,并進(jìn)而在小鼠身上進(jìn)行了皮膚損傷修復(fù)實驗,取得了令人滿意的修復(fù)效果。
[Abstract]:The basic principle of skin tissue engineering is to combine the expanded skin seed cells in vitro with materials with good biocompatibility to establish a three-dimensional complex between cells and biomaterials, the basic elements of which include seed cells. Scaffold materials and tissue construction. With the advantages of controllable pore structure, wide size range and high production capacity, the technology of biologic 3D printing can accurately locate many kinds of matrix materials and cells. It has great potential in the construction of tissue engineering skin. In this dissertation, the printing experiments of hydrogel scaffolds and skin cells were carried out by using the biological three-dimensional printing system developed by ourselves. Based on the analysis of the basic characteristics of the hydrated material, the extrusion process of the material was calculated and simulated. At the same time, the experiments of skin tissue engineering scaffold and cell printing were carried out by 3D printing. The results show that the system has good scaffold forming ability, and the constructed tissue scaffold can provide a suitable three-dimensional growth environment for cells. The main work of this paper is as follows: 1. Design and development of three-dimensional printing system for skin tissue engineering. A flexible printing scheme based on pneumatic extrusion principle was proposed based on the basic principle of biological 3D printing technology and the specific requirements of skin tissue engineering. The system is composed of five parts: motion system, temperature control system, cross-linking system and environmental control system. It can be used in many biomaterials and cell printing research. Second, analysis of extrusion process of hydrogel material. Based on the constitutive equation of the power law fluid, the mathematical models of the fiber width, the height of the scaffold layer and the porosity of the hydrogel material after extrusion from the nozzle are established, and the flow velocity and wall shear stress during extrusion are analyzed by means of fluid simulation. The phenomenon of extrusion swell is analyzed, which provides theoretical support and guidance for the control and optimization of printing parameters. Third, skin tissue engineering scaffold design and printing. According to the structural characteristics of human skin, the three-dimensional model of skin scaffold is designed, and the printing algorithm is studied from the aspects of polygon contour feature recognition, scanning intersection, regional grouping, path filling and so on. The ACSPL language is used to design the scaffold printing program, and finally, the 3D scaffold structure with different structure parameters is printed out. Fourth, seed cell printing and three-dimensional culture. Skin fibroblasts were selected as seed cells for cell printing and three dimensional culture. The activity of the cells after printing and in culture was analyzed by fluorescence dye method. The growth, proliferation and migration of fibroblasts in three dimensional hydrogel scaffolds were observed, and skin injury repair experiments were carried out in mice. Satisfactory results were obtained.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R318.1;TP391.73

【參考文獻(xiàn)】

相關(guān)期刊論文 前10條

1 陳鳳超;陳民才;顏彤彤;曾燕;楊俊革;;鼠胚胎成纖維細(xì)胞三維培養(yǎng)修復(fù)大鼠全層皮膚缺損的效果及其機(jī)制[J];中國醫(yī)藥導(dǎo)報;2016年30期

2 楊維;崔占峰;;組織工程皮膚發(fā)展現(xiàn)狀[J];中國科學(xué):生命科學(xué);2015年05期

3 何鳳利;何進(jìn);尹大川;;靜電紡絲制備組織工程支架的研究進(jìn)展[J];材料導(dǎo)報;2014年23期

4 于永澤;陳海萍;胡慶夕;;生物凝膠微孔擠出脹大的有限元模擬與三維打印成形[J];機(jī)械工程學(xué)報;2014年19期

5 胡玎玎;吳振飛;劉小琨;顏世峰;尹靜波;;皮膚組織工程支架材料的研究進(jìn)展[J];高分子通報;2012年10期

6 何進(jìn);郭云珠;曹慧玲;葉雅靜;尹大川;;組織工程支架研究進(jìn)展[J];材料導(dǎo)報;2012年03期

7 付明福;楊影;陳偉才;廖新根;戴鵬;張立超;羅軍;;噴墨打印技術(shù)同步打印細(xì)胞和生物支架材料及在組織工程中的應(yīng)用[J];中國組織工程研究與臨床康復(fù);2011年42期

8 李生杰;顏永年;王小紅;張婷;張人佶;林峰;;多噴頭細(xì)胞受控組裝過程的啟停響應(yīng)控制[J];電加工與模具;2010年01期

9 陳思詩;楊慶;沈新元;郯志清;彭蘭蘭;;溶劑澆鑄-粒子瀝濾法制備PBS/PCL組織工程支架[J];東華大學(xué)學(xué)報(自然科學(xué)版);2009年04期

10 胡康洪;姚穎;;三維細(xì)胞培養(yǎng)技術(shù)的研究與應(yīng)用[J];醫(yī)學(xué)分子生物學(xué)雜志;2008年02期

，

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