本文選題：量子點　切入點：巨噬細(xì)胞　出處：《中國組織工程研究》2017年26期

【摘要】：背景:與傳統(tǒng)的有機(jī)熒光染料相比,量子點呈現(xiàn)出良好的生物標(biāo)記特性,尤其在細(xì)胞標(biāo)記、臨床靶向生物成像等領(lǐng)域呈現(xiàn)出獨特的光學(xué)特性。目的:觀察CdS e/Zn S量子點與單核-巨噬細(xì)胞的生物相容性。方法:將巨噬細(xì)胞RAW264.7接種于96孔板中,分3組培養(yǎng),分別加入0,50,100 mg/L的Cd Se/Zn S量子點干預(yù),培養(yǎng)1 h或2 h,流式細(xì)胞儀檢測熒光信號;培養(yǎng)0-24 h,流式細(xì)胞儀檢測50 mg/L Cd Se/ZnS量子點標(biāo)記巨噬細(xì)胞的熒光信號強(qiáng)度;培養(yǎng)18 h,定量PCR檢測巨噬細(xì)胞內(nèi)腫瘤壞死因子α及白細(xì)胞介素1β的水平;培養(yǎng)18 h,MTT法檢測巨噬細(xì)胞增殖,流式細(xì)胞儀檢測細(xì)胞凋亡。結(jié)果與結(jié)論:(1)熒光信號強(qiáng)度與CdS e/Zn S量子點的質(zhì)量濃度呈正相關(guān),并且隨著標(biāo)記時間的延長,熒光信號強(qiáng)度增強(qiáng);(2)經(jīng)50 mg/L CdS e/ZnS量子點標(biāo)記后,隨著時間的延長,巨噬細(xì)胞的熒光信號不斷增強(qiáng),在18 h達(dá)到峰值;(3)與0 mg/L量子點組比較,50,100 mg/L量子點組可顯著促進(jìn)巨噬細(xì)胞內(nèi)腫瘤壞死因子α及白細(xì)胞介素1β的表達(dá)(P0.01或P0.05);100 mg/L量子點組腫瘤壞死因子α表達(dá)高于50 mg/L量子點組(P0.01);50,100 mg/L量子點組白細(xì)胞介素1β表達(dá)無差異;(4)50,100 mg/L Cd Se/Zn S量子點組細(xì)胞增殖強(qiáng)于0 mg/L量子點組(P0.05),50,100 mg/L Cd Se/Zn S量子點組細(xì)胞增殖無差異;(5)50,100 mg/L Cd Se/Zn S量子點對巨噬細(xì)胞的凋亡無明顯影響;(6)結(jié)果表明,Cd Se/Zn S量子點可活化巨噬細(xì)胞,促其增殖與分泌炎癥因子,但不影響其凋亡。
[Abstract]:Background: compared with traditional organic fluorescent dyes, quantum dots (QDs) exhibit good biomarker properties, especially in cell labeling, clinical targeted bioimaging and other fields.Aim: to observe the biocompatibility of CdS e/Zn S quantum dots with mononuclear macrophages.Methods: macrophage RAW264.7 was inoculated into 96-well plate and cultured in three groups. The macrophages were incubated with 0 ~ 50100 mg/L CD Se/Zn S quantum dots for 1 h or 2 h, and fluorescence signals were detected by flow cytometry.The fluorescence signal intensity of macrophages labeled with 50 mg/L CD Se/ZnS quantum dots was detected by flow cytometry at 0-24 h, and the levels of tumor necrosis factor 偽 and interleukin-1 尾 in macrophages were measured by quantitative PCR at 18 h.The proliferation of macrophages was detected by MTT assay and apoptosis was detected by flow cytometry.The results showed that the intensity of fluorescence signal was positively correlated with the mass concentration of CdS e/Zn S quantum dots, and the fluorescence signal intensity was enhanced with the prolongation of labeling time.The fluorescence signals of macrophages are increasing.The expression of tumor necrosis factor 偽 and interleukin-1 尾 in macrophages was significantly increased in 50 mg/L QDs group than that in 0 mg/L QDs group. The expression of TNF 偽 in macrophages was significantly higher than that in P0.01 or P0.05 mg/L QDs group.mg/L閲忓瓙鐐圭粍(P0.01);50,100 mg/L閲忓瓙鐐圭粍鐧界粏鑳?yōu)浠嬬矗?

本文編號：1707284