本文選題：高分子生物工程支架　切入點(diǎn)：三維多孔結(jié)構(gòu)　出處：《鄭州大學(xué)》2012年博士論文　論文類型：學(xué)位論文

【摘要】：組織工程多孔生物支架作為種植細(xì)胞的場(chǎng)所和組織再生的模板,是組織工程領(lǐng)域重要研究方向之一,已經(jīng)引起了越來(lái)越多的研究者重視。理想的多孔生物支架必須具備,高孔隙率、內(nèi)部相互連通性、無(wú)毒性、良好的生物相容性和較好力學(xué)性能等,因此,如何制備符合及滿足細(xì)胞需求的理想三維多孔生物支架是組織工程領(lǐng)域的前沿課題。基于可降解高分子生物支架具有在引導(dǎo)細(xì)胞生長(zhǎng)的同時(shí)可被機(jī)體吸收的突出優(yōu)點(diǎn),使其成為生物支架研究方向的熱點(diǎn)。 論文以可降解高分子生物支架的制備工藝一結(jié)構(gòu)形態(tài)一性能為主線,通過(guò)選擇合適的加工方法、設(shè)計(jì)合理的材料配方,調(diào)控泡孔形態(tài)、孔隙率、吸水率、內(nèi)部連通性和力學(xué)性能等,加工制備新型的具有高孔隙率且內(nèi)部相互連通的三維多孔可降解高分子生物支架。主要工作包括： 1、采用注塑成型／粒子瀝濾技術(shù)加工PCL和PCL/HA三維多孔生物支架�；诓煌牧吓浞娇刂浦Ъ艿呐菘仔螒B(tài)、孔隙率、力學(xué)性能、吸水性能和生物性能。分析NaCl顆粒和可溶性高分子聚合物PEO的瀝濾過(guò)程,對(duì)比探討了PCL和PCL/HA三維多孔生物支架的力學(xué)性能和生物相容性。研究結(jié)果表明,PEO的應(yīng)用較大的提高了支架內(nèi)部相互連通性；HA的使用不僅增強(qiáng)了支架的力學(xué)性能,而且促進(jìn)了細(xì)胞的增殖生長(zhǎng)。 2、利用微發(fā)泡注塑成型/粒子瀝濾技術(shù)加工PCL三維多孔生物支架。對(duì)比探究了傳統(tǒng)注塑成型和微發(fā)泡注塑成型技術(shù)所制備支架的內(nèi)部孔洞結(jié)構(gòu)形態(tài)、靜態(tài)和動(dòng)態(tài)力學(xué)性能、孔隙率和吸水率等。超臨界N2的使用不僅作為物理發(fā)泡劑,而且起到增塑劑的作用,降低共混物的熔體粘度,使其易于加工成型,同時(shí)有效的提高了PCL生物支架的孔隙率。 3、基于注塑成型技術(shù)、弱酸瀝濾方法和新材料配方(PCL/PEO/NaCl/殼聚糖纖維),首次利用殼聚糖纖維加工了具有微孔洞和微管道雙重形態(tài)特征的三維多孔PCL生物支架。殼聚糖纖維的引入,不僅在支架中形成了微管道結(jié)構(gòu)形態(tài),而且提高了其孔隙率及內(nèi)部連通性。分析了殼聚糖含量對(duì)PCL生物支架泡孔形態(tài)和力學(xué)性能影響。 4、采用單向冷凍干燥法和靜電紡絲技術(shù)相結(jié)合,加工了具有微米級(jí)管道和納米級(jí)微結(jié)構(gòu)特征的高孔隙率三維殼聚糖/PLGA納米復(fù)合生物支架。相比殼聚糖生物支架,PLGA納米纖維的加入不僅使三維生物支架具有納米級(jí)形態(tài)結(jié)構(gòu),而且使其具有較大的比表面積和更高的力學(xué)性能。并研究了殼聚糖溶液濃度和靜電紡絲時(shí)間對(duì)支架結(jié)構(gòu)形態(tài)和力學(xué)性能的影響。 5、研究了壓縮成型聚酸酐樣品的降解機(jī)理和降解速率。分析探究了樣品加工溫度和幾何尺寸對(duì)其降解速率的影響。
[Abstract]:Tissue engineering porous scaffold, as a template for cell implantation and tissue regeneration, is one of the important research directions in the field of tissue engineering, and has attracted more and more researchers' attention. High porosity, internal connectivity, non-toxicity, good biocompatibility and good mechanical properties, etc. How to prepare ideal three-dimensional porous scaffolds that meet and meet the needs of cells is a leading issue in tissue engineering. Based on the outstanding advantages of biodegradable polymer scaffolds, they can be absorbed by the body while guiding cell growth. It has become a hot spot in the research direction of biological scaffolds. In this paper, the preparation process of biodegradable polymer scaffolds, structure, morphology and properties are taken as the main line. By selecting suitable processing methods, a reasonable material formulation is designed to regulate the bubble morphology, porosity and water absorption. New three dimensional porous biodegradable polymer scaffolds with high porosity and interconnectedness are fabricated by internal connectivity and mechanical properties. The main work includes:. 1. PCL and PCL/HA three-dimensional porous biological scaffolds were fabricated by injection molding / particle leaching technology. The bubble morphology, porosity and mechanical properties of the scaffolds were controlled based on different material formulations. Water absorption and biological properties. Analysis of leaching process of NaCl particles and soluble polymer PEO, The mechanical properties and biocompatibility of PCL and PCL/HA three-dimensional porous scaffolds were compared and discussed. The results showed that the application of PCL/HA not only enhanced the mechanical properties of scaffolds, but also improved the internal connectivity of the scaffolds. And promote cell proliferation and growth. 2. Microfoaming injection molding / particle leaching technology was used to process PCL three-dimensional porous biological scaffolds. The internal pore structure, static and dynamic mechanical properties of the scaffolds prepared by conventional injection molding and micro-foaming injection molding were compared. The use of supercritical N2 is not only used as a physical foaming agent but also as a plasticizer to reduce the melt viscosity of the blend and make it easy to be processed and molded. At the same time the porosity of the PCL biological scaffold is improved effectively. 3. Based on injection molding technology, weak acid leaching method and new material formula, PCL / PEO / NaCl / chitosan fiber was used for the first time to process three-dimensional porous PCL biological scaffolds with dual morphology of micropores and microtubes. Not only the microtube structure was formed in the scaffold, but also the porosity and internal connectivity were improved. The effect of chitosan content on the bubble morphology and mechanical properties of PCL biological scaffold was analyzed. (4) unidirectional freeze-drying method and electrostatic spinning technology, Three dimensional chitosan / PLGA nanocomposite scaffolds with high porosity and microstructures were fabricated. Compared with chitosan scaffolds, the addition of PLGA nanofibers not only made the three-dimensional scaffolds have nanoscale morphology. The effects of concentration of chitosan solution and electrostatic spinning time on the structure morphology and mechanical properties of the scaffold were studied. 5. The degradation mechanism and degradation rate of compression formed polyanhydride samples were studied, and the effects of sample processing temperature and geometric size on the degradation rate were analyzed.
【學(xué)位授予單位】：鄭州大學(xué)
【學(xué)位級(jí)別】：博士
【學(xué)位授予年份】：2012
【分類號(hào)】：R318.08

【共引文獻(xiàn)】

相關(guān)期刊論文 前2條

1 王澤云;崔愛(ài)軍;陸衛(wèi)良;陳群;何明陽(yáng);;熔融/固相縮聚法制備聚乙醇酸甲酯的工藝條件優(yōu)化[J];化工進(jìn)展;2012年12期

2 崔志香;PENG Yiyan;LI Ke;PENG Jun;ZHAO Haibin;TURNG Lih-Sheng;SHEN Changyu;;The Degradation Rate of Polyanhydride (Poly(sebacic acid), diacetoxy terminated, PSADT)[J];Journal of Wuhan University of Technology(Materials Science Edition);2013年04期

相關(guān)博士學(xué)位論文 前1條

1 趙海濱;微孔發(fā)泡注射成型制備三維多孔聚乳酸骨組織工程支架研究[D];華南理工大學(xué);2013年

相關(guān)碩士學(xué)位論文 前2條

1 苗貴強(qiáng);聚左旋乳酸/殼聚糖納米纖維三維多孔支架復(fù)合骨髓間充質(zhì)干細(xì)胞修復(fù)骨缺損的實(shí)驗(yàn)研究[D];暨南大學(xué);2011年

2 熊高鑫;聚碳酸亞丙酯/殼聚糖納米纖維復(fù)合三維多孔支架修復(fù)骨缺損的實(shí)驗(yàn)研究[D];暨南大學(xué);2010年

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本文編號(hào)：1566648