發(fā)布時(shí)間：2018-12-17 21:47

【摘要】：本文利用多光子顯微成像技術(shù)的先進(jìn)手段,以亞微米尺度觀察在紫外燈輻照下的老化皮膚在不同光源的作用下皮膚膠原重構(gòu)的情況,揭示光不同光源與生物組織相互作用產(chǎn)生的效果,進(jìn)一步地理解老化皮膚在激光作用下的機(jī)制。同時(shí)利用OCT的高分辨率和非侵入式測(cè)量的特點(diǎn),對(duì)不同光源的嫩膚效果進(jìn)行初步比較,得到光老化皮膚在嫩膚過(guò)程中的結(jié)構(gòu)改變和散射系數(shù)的變化情況。 首先,介紹了光美容技術(shù)發(fā)展的歷史和現(xiàn)狀,論述了光美容治療過(guò)程中的療效評(píng)價(jià)手段以及OCT技術(shù)、多光子顯微技術(shù)在光美容診斷上的應(yīng)用及發(fā)展,闡述了本課題的研究意義。同時(shí),對(duì)皮膚的結(jié)構(gòu)進(jìn)行簡(jiǎn)要的介紹,討論了光子嫩膚的作用機(jī)理、劑量選擇的基本原理。并結(jié)合本文實(shí)際使用的儀器,探討儀器應(yīng)用的可行性和使用方式。 其次,利用多光子顯微成像技術(shù),從亞微米數(shù)量級(jí)觀察不同光源對(duì)皮膚的作用效果,分析真皮層膠原纖維的變化情況,并結(jié)合HE染色切片進(jìn)行觀察。 最后,采用了OCT系統(tǒng)比較非消融性的IPL和消融性的Er：YAG激光的作用效果。觀察不同光源及不同能量密度作用下的生物組織體現(xiàn)的散射光強(qiáng)以及散射系數(shù)之間存在著明顯變化和區(qū)別。
[Abstract]:In this paper, we use the advanced technique of multiphoton microscopic imaging to observe the collagen remodeling of aging skin irradiated by ultraviolet lamp under different light sources at sub-micron scale. The effects of interaction between different light sources and biological tissues were revealed, and the mechanism of aging skin under laser irradiation was further understood. At the same time, the characteristics of high resolution and non-invasive measurement of OCT are used to compare the skin effect of different light sources, and the changes of structure and scattering coefficient of photoaged skin in the process of skin rejuvenation are obtained. Firstly, this paper introduces the history and present situation of the development of light beauty technology, discusses the evaluation method of the curative effect in the process of light beauty therapy, the application and development of OCT technology and multiphoton microscopy technology in the diagnosis of light beauty, and expounds the research significance of this subject. At the same time, the structure of skin is briefly introduced, and the mechanism of photon tender skin and the basic principle of dose selection are discussed. Combined with the instrument used in this paper, the feasibility and application of the instrument are discussed. Secondly, the effects of different light sources on the skin were observed by using multi-photon microscopic imaging technique, and the changes of collagen fibers in the dermis were analyzed, and the changes of collagen fibers in the dermis were observed by HE staining. Finally, OCT system is used to compare the effect of nonablative IPL and ablative Er:YAG laser. The variation and difference of scattering intensity and scattering coefficient of biological tissue under different light sources and different energy density were observed.
【學(xué)位授予單位】：福建師范大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2010
【分類(lèi)號(hào)】：R758.1

【引證文獻(xiàn)】

相關(guān)碩士學(xué)位論文 前2條

1 徐小暉;小鼠皮膚深Ⅱ度燙傷及治療的光學(xué)觀察與分析[D];福建師范大學(xué);2012年

2 陳新光;檢測(cè)神經(jīng)纖維膜電位動(dòng)力學(xué)的光學(xué)二次諧波編碼及其數(shù)值模擬[D];福建師范大學(xué);2012年

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本文編號(hào)：2384839