【摘要】： 目的：觀察RECK基因及基質(zhì)金屬蛋白酶-9(MMP-9)在皮膚鱗狀細(xì)胞癌(CSCC)中的表達(dá),探討其與皮膚鱗狀細(xì)胞癌發(fā)生發(fā)展及浸潤轉(zhuǎn)移的關(guān)系。 方法：應(yīng)用免疫組化的方法檢測40例CSCC組織、17例癌旁非典型增生組織及14例正常皮膚組織中RECK及MMP-9蛋白的表達(dá)。實(shí)驗(yàn)結(jié)果采用SPSS11.5統(tǒng)計(jì)學(xué)軟件進(jìn)行x2檢驗(yàn)和Spearman等級相關(guān)性分析。 結(jié)果：①RECK在CSCC、癌旁不典型增生及正常皮膚中的表達(dá)率依次增高(30.0%、47.1%、85.7%),組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05); MMP-9蛋白在CSCC、癌旁不典型增生及正常皮膚中的表達(dá)率依次降低(82.5%、76.4%、28.6%),組間比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)②RECK及MMP-9與CSCC的組織學(xué)分級、淋巴結(jié)轉(zhuǎn)移密切相關(guān)(P均0.05)；與CSCC患者的性別、年齡無關(guān)(P均0.05)③RECK及MMP-9在CSCC中的表達(dá)呈負(fù)相關(guān)(r=-0.475 P=0.002)。 結(jié)論：RECK和MMP-9與CSCC的浸潤轉(zhuǎn)移密切相關(guān),RECK在CSCC中表達(dá)減少或缺失可能是通過上調(diào)MMP-9的表達(dá)從而促進(jìn)CSCC的侵襲與轉(zhuǎn)移。
[Abstract]:Objective: to investigate the expression of RECK gene and matrix metalloproteinase-9 (MMP-9) in cutaneous squamous cell carcinoma (SCC) (CSCC), and to explore the relationship between (CSCC) expression and invasion and metastasis of cutaneous squamous cell carcinoma (SCC). Methods: immunohistochemical method was used to detect the expression of RECK and MMP-9 protein in 40 cases of CSCC tissues and 17 cases of adjacent atypical hyperplasia tissues and 14 cases of normal skin tissues. The results of the experiment were analyzed by using SPSS11.5 statistical software for x 2 test and Spearman grade correlation analysis. Results the expression rate of MMP-9 protein in CSCC, paracancerous dysplasia and normal skin increased in turn (30.0%, 47.1%, 85.7%), the difference was statistically significant (P0.05), and the expression rate of MMP-9 protein in CSCC, paracancerous dysplasia and normal skin decreased in turn (82.576.445%). The difference was statistically significant (P0.05) the histological grade of 2RECK, MMP-9 and CSCC, The expression of 3RECK and MMP-9 in CSCC was negatively correlated with the sex and age of patients with CSCC (P 0. 05) (P 0. 05), and negatively correlated with the expression of 3RECK and MMP-9 in CSCC (r 0. 475 P0. 002). Conclusion the decrease or deletion of MMP-9 expression in CSCC may promote the invasion and metastasis of CSCC by upregulating the expression of MMP-9, which is closely related to the invasion and metastasis of CSCC.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2010
【分類號】：R739.5

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