【摘要】：目的 為了了解表皮非黑素腫瘤中干細(xì)胞特異性標(biāo)志物Oct-4的表達(dá)情況以及在表皮腫瘤發(fā)生中的作用。 方法 本研究選擇20例人皮膚鱗狀細(xì)胞癌(SCC)、20例基底細(xì)胞癌(BCC)、20例脂溢性角化(SK)和20例正常人皮膚組織,采用EnVision免疫組化技術(shù)觀察這些組織中干細(xì)胞特異性標(biāo)志物Oct-4和增殖細(xì)胞核抗原(PCNA)的表達(dá)情況。 結(jié)果 ①Oct-4在皮膚SCC、BCC中表達(dá)強(qiáng)度與正常皮膚組織和皮膚SK相比明顯增高(P0.05)，，Oct-4在皮膚SCC和BCC之間的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)。Oct-4在皮膚SK和正常皮膚組織之間的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05)。Oct-4在皮膚SK和BCC之間的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05).②PCNA在皮膚SCC、BCC中表達(dá)強(qiáng)度與正常皮膚組織和皮膚SK比較具有統(tǒng)計(jì)學(xué)意義(P0.05)；在皮膚SCC和BCC之間的表達(dá)差異具有統(tǒng)計(jì)學(xué)意義(P0.05)；在皮膚SK和正常皮膚組織之間的表達(dá)差異也具有統(tǒng)計(jì)學(xué)意義(P0.05)；而PCNA在皮膚SK和BCC之間的表達(dá)差異無統(tǒng)計(jì)學(xué)意義(P0.05).③Oct-4在皮膚SCC.BCC中表達(dá)強(qiáng)度與細(xì)胞周期蛋白PCNA表達(dá)陽性強(qiáng)度均呈正相關(guān),P0.05。 結(jié)論 ①干細(xì)胞特異性標(biāo)志物Oct-4在BCC和SCC中表達(dá)強(qiáng)度明顯增高,可能與BCC和SCC發(fā)生發(fā)展有關(guān)。②PCNA在BCC和SCC中強(qiáng)陽性表達(dá),可能參與了BCC和SCC增殖過程。③Oct-4和PCNA在皮膚SCC.BCC中的陽性表達(dá)強(qiáng)度呈正相關(guān)性,Oct-4可能通過影響細(xì)胞增殖參與了BCC和SCC的發(fā)病過程。
[Abstract]:Objective to investigate the expression of stem cell specific marker Oct-4 in epidermal non-melanoma and its role in the pathogenesis of epidermal neoplasms. Methods 20 cases of human cutaneous squamous cell carcinoma (SCC), 20 cases of basal cell carcinoma (BCC), 20 cases of seborrheic keratosis (SK) and 20 cases of normal human skin tissue were studied. The expression of stem cell specific marker Oct-4 and proliferating cell nuclear antigen (PCNA) in these tissues was observed by envision immunohistochemical technique. Results 1 the expression intensity of Oct-4 in skin SCC+ BCC was significantly higher than that in normal skin tissue and skin SK (P0.05). There was no significant difference in the expression of Oct-4 between skin SCC and BCC (P0.05) .Oct-4 in SK and normal skin tissue. There was no significant difference in expression of Oct-4 between skin SK and BCC (P0.05). 2 the expression intensity of PCNA in skin SCCU BCC was significantly higher than that in normal skin tissue and skin SK (P0.05). There was significant difference between SCC and BCC in skin (P0.05) and between SK and normal skin (P0.05). However, there was no significant difference in the expression of PCNA between SK and BCC (P0.05) .3Oct-4 in skin SCC.BCC was positively correlated with the positive expression of cyclin PCNA (P 0.05). Conclusion (1) the expression of Oct-4 in BCC and SCC is significantly higher than that in BCC and SCC, which may be related to the strong positive expression of PCNA in BCC and SCC. Oct-4 may be involved in the proliferation of BCC and SCC. 3Oct-4 and PCNA in the skin SCC.BCC positive expression intensity positive correlation may affect the proliferation of BCC and SCC may be involved in the pathogenesis of BCC and SCC.
【學(xué)位授予單位】：浙江大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2011
【分類號(hào)】：R739.5

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