本文選題：PM. + 角質(zhì)形成細(xì)胞　； 參考：《中國皮膚性病學(xué)雜志》2017年07期

【摘要】：目的研究空氣細(xì)顆粒物(PM2.5)對人皮膚角質(zhì)形成細(xì)胞自噬水平的影響。方法收集武漢市區(qū)空氣中的PM2.5,分離并提取其水溶性及非水溶性成分,以不同的終濃度處理人永生化角質(zhì)形成細(xì)胞(HaCaT細(xì)胞)。利用細(xì)胞免疫熒光染色和Western印跡法檢測人角質(zhì)形成細(xì)胞中自噬相關(guān)蛋白LC3的表達(dá)。結(jié)果細(xì)胞免疫熒光結(jié)果表明,經(jīng)PM2.5染毒液誘導(dǎo)的細(xì)胞LC3表達(dá)量均高于對照組,且呈濃度依賴性升高。Western印跡檢測結(jié)果顯示,實驗組細(xì)胞LC3-II/I比值明顯高于對照組(P0.05,除B1組);相同濃度下,PM2.5水溶性成分與非水溶性成分比較作用強(qiáng)度差異無統(tǒng)計學(xué)意義(P0.05)。結(jié)論 PM2.5可誘導(dǎo)人皮膚角質(zhì)形成細(xì)胞發(fā)生自噬,并與濃度有明顯相關(guān)性,其誘導(dǎo)的機(jī)制可能是細(xì)胞對抗氧化應(yīng)激損傷及細(xì)胞凋亡的一種自我保護(hù)反應(yīng)。
[Abstract]:Objective to study the effect of PM2.5) on autophagy of human skin keratinocytes. Methods PM2.5 was collected from air of Wuhan urban area and its water-soluble and insoluble components were isolated and extracted. HaCaT cells were treated with different final concentrations of human immortalized keratinocytes. The expression of autophagy associated protein LC3 in human keratinocytes was detected by immunofluorescence staining and Western blot. Results the results of cellular immunofluorescence showed that the level of LC3 expression induced by PM2.5 was higher than that in control group, and the expression of LC3 was increased in a concentration-dependent manner. Western blot analysis showed that the expression of LC3 was increased in a concentration dependent manner. The ratio of LC3-II-1 / I in the experimental group was significantly higher than that in the control group (P 0.05, except group B1), and there was no significant difference in the action intensity between the water-soluble component of PM2.5 and the non-water-soluble component at the same concentration. Conclusion PM2.5 can induce autophagy of human skin keratinocytes and has a significant correlation with the concentration. The mechanism of PM2.5 may be a self-protective response of cells to antioxidant stress injury and apoptosis.
【作者單位】： 武漢大學(xué)附屬中南醫(yī)院皮膚性病科;
【分類號】：R751
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本文編號：1994535