本文選題：特應(yīng)性皮炎 + TSLP�。� 參考：《瀘州醫(yī)學(xué)院》2012年碩士論文

【摘要】：目的：通過建立小鼠特應(yīng)性皮炎模型,檢測(cè)胸腺基質(zhì)淋巴細(xì)胞生成素(thymic stromal lymphopoietin, TSLP)在小鼠皮損及血清中的表達(dá)以及白介素-4(interleukin-4, IL-4)、γ干擾素(interferon-γ,IFN-γ)和免疫球蛋白E (immunoglobulin-E, IgE)在小鼠血清中的表達(dá)水平,分析TSLP與IL-4、IFN-γ和IgE的血清表達(dá)水平的相關(guān)性。探討TSLP在特應(yīng)性皮炎發(fā)病機(jī)制中的作用。方法：采用隨機(jī)對(duì)照實(shí)驗(yàn)方法將18只BALB/c小鼠隨機(jī)分入實(shí)驗(yàn)組和對(duì)照組。實(shí)驗(yàn)組用2.4-二硝基氟苯(2,4-Dinitrofluorobenzene, DNFB)丙酮/橄欖油(3：1)溶液致敏與激發(fā)建立小鼠特應(yīng)性皮炎模型；對(duì)照組僅用丙酮/橄欖油(3：1)溶液。用蘇木素-伊紅(HE)染色方法觀察兩組小鼠皮損病理變化,并采用免疫組化技術(shù)檢測(cè)TSLP在兩組小鼠皮損組織中的表達(dá),用酶聯(lián)免疫吸附試驗(yàn)(Enzyme-linked immunosorbent assay, ELISA)方法檢測(cè)兩組小鼠血清中TSLP、IL-4、IFN-γ及IgE的表達(dá)水平。用SPSS17.0統(tǒng)計(jì)軟件包對(duì)資料進(jìn)行統(tǒng)計(jì)分析,計(jì)量資料比較用t檢驗(yàn),指標(biāo)間的相關(guān)性用spearman直線相關(guān)分析。結(jié)果：1.小鼠皮損表現(xiàn)：實(shí)驗(yàn)組小鼠皮膚可見紅斑、丘疹、結(jié)痂、苔蘚樣變等皮損改變；對(duì)照組小鼠皮膚表現(xiàn)正常。2.皮損組織學(xué)變化：2.1皮損組織病理表現(xiàn)：實(shí)驗(yàn)組見表皮輕度角化過度伴角化不全,棘層肥厚,棘細(xì)胞間海綿水腫,真皮淺層以淋巴細(xì)胞為主的慢性炎細(xì)胞浸潤；對(duì)照組組織結(jié)構(gòu)基本正常,未見明顯炎性細(xì)胞浸潤。2.2皮損組織免疫組化表現(xiàn)：TSLP陽性表達(dá)物質(zhì)主要位于胞核,胞漿及胞膜無特異性著色,實(shí)驗(yàn)組平均光密度值(74.306±17.898)明顯高于對(duì)照組(46.093±3.347),差異有統(tǒng)計(jì)學(xué)意義(p0.05)。3.TSLP、IL-4、IFN-γ和IgE血清學(xué)水平比較：3.1 TSLP：實(shí)驗(yàn)組血清TSLP水平為213.621 ± 34.666pg/ml,對(duì)照組血清TSLP水平為151.997±22.328pg/m1,實(shí)驗(yàn)組血清TSLP水平顯著高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。3.2IL-4：實(shí)驗(yàn)組血清IL-4水平為97.831±15.882pg/ml,對(duì)照組血清IL-4水平為60.56l±9.286pg/ml,實(shí)驗(yàn)組血清工L-4水平顯著高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。3.3 IFN-γ：實(shí)驗(yàn)組血清IFN-γ水平為70.999±9.064pg/ml,對(duì)照組血清IFN-γ水平為51.224±4.534 pg/ml,實(shí)驗(yàn)組血清IFN-γ水平顯著高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。3.4 IgE：實(shí)驗(yàn)組血清IgE水平為142.488±16.352ng/m1,對(duì)照組血清IgE水平為14.851±3.971ng/ml,實(shí)驗(yàn)組工gE水平顯著高于對(duì)照組,差異有統(tǒng)計(jì)學(xué)意義(p0.05)。4.實(shí)驗(yàn)組血清TSLP與IL-4、IFN-γ及IgE相關(guān)性分析：實(shí)驗(yàn)組TSLP血清水平與IL-4及IgE血清水平呈正相關(guān)(p0.05),而與IFN-γ血清水平呈負(fù)相關(guān)(p0.05)。結(jié)論：1.采用DNFB致敏和激發(fā)小鼠皮膚表面能夠成功建立小鼠特應(yīng)性皮炎模型。2.TSLP在特應(yīng)性皮炎小鼠皮損組織及血清中大量表達(dá),TSLP可促進(jìn)IL-4及IgE的表達(dá),而抑制IFN-γ的表達(dá),其在特應(yīng)性皮炎的發(fā)病機(jī)制中可能起著重要作用。3.特應(yīng)性皮炎小鼠血清中IL-4、IFN-γ及IgE水平升高,這些細(xì)胞因子在特應(yīng)性皮炎的發(fā)病過程中可能扮演著重要角色。
[Abstract]:Objective: to determine the expression of thymic stromal lymphopoietin (TSLP) in the skin lesions and serum of mice, and the expression of interleukins -4 (interleukin-4, IL-4), interferon (interferon- y, IFN- gamma) and immunoglobulin E (immunoglobulin-E, IgE) in the mice serum by establishing a mouse atopic dermatitis model. The correlation between TSLP and the serum levels of IL-4, IFN- gamma and IgE was analyzed. The role of TSLP in the pathogenesis of atopic dermatitis was investigated. Methods: 18 BALB/c mice were randomly divided into experimental and control groups by random control experiment. The experimental group was treated with 2.4- two nitrofluoro (2,4-Dinitrofluorobenzene, DNFB) acetone / olive oil. (3:1) a mouse atopic dermatitis model was established by solution sensitization and stimulation; the control group only used acetone / olive oil (3:1) solution. The pathological changes of skin lesions in two groups of mice were observed with hematoxylin eosin (HE) staining, and the expression of TSLP in the skin lesions of two groups of mice was detected by immunohistochemical technique, and the enzyme linked immunosorbent assay (Enzyme-linked Immunosorbent assay, ELISA) method was used to detect the expression level of TSLP, IL-4, IFN- gamma and IgE in the serum of two groups of mice. The data were statistically analyzed with SPSS17.0 statistical package, the measurement data were compared with t test, and the correlation between the indexes was analyzed by Spearman linear correlation. Results: 1. mice skin lesions showed red spots in the skin of the experimental mice. Changes in skin lesions such as papules, scab and moss like changes in the skin of the control group; the pathological changes of normal.2. skin lesions in the control group of mice: 2.1 lesions in the skin lesions: mild keratinization and keratinization in the experimental group, spinous cell hypertrophy, spongedema between spinous cells, and chronic lymphocytic infiltration of superficial lymphoblastic cells in the superficial layer of the dermis; the control group. The structure was normal and no obvious inflammatory cell infiltration of.2.2 skin tissue was found. The positive expression of TSLP was mainly located in the nucleus, and the cytoplasm and membrane had no specific coloring. The average optical density (74.306 + 17.898) of the experimental group was significantly higher than that of the control group (46.093 + 3.347). The difference was statistically significant (P0.05).3.TSLP, IL-4, IFN- gamma and IgE Serological comparison: 3.1 TSLP: the level of serum TSLP in the experimental group was 213.621 + 34.666pg/ml, and the level of serum TSLP in the control group was 151.997 + 22.328pg/m1. The serum TSLP level of the experimental group was significantly higher than that of the control group. The difference was statistically significant (P0.05).3.2IL-4: the serum IL-4 water level was 97.831 + 15.882pg/ml in the experimental group, and the serum IL-4 level in the control group. For 60.56l + 9.286pg/ml, the serum level of L-4 in the experimental group was significantly higher than that of the control group. The difference was statistically significant (P0.05).3.3 IFN- Gamma: the serum IFN- gamma level was 70.999 + 9.064pg/ml in the experimental group and the level of IFN- gamma in the control group was 51.224 + 4.534 pg/ml. The serum IFN- gamma level in the experimental group was significantly higher than that in the control group. The difference was statistically significant (P0.05). IgE: the level of serum IgE in the experimental group was 142.488 + 16.352ng/m1, the level of serum IgE in the control group was 14.851 + 3.971ng/ml, and the level of gE in the experimental group was significantly higher than that of the control group. The difference was statistically significant (P0.05) in the experimental group (P0.05), the serum TSLP and IL-4, IFN- gamma and IgE correlation analysis: the serum level of the experimental group was positively correlated with the serum level and the serum level. .05) and negative correlation with IFN- gamma serum level (P0.05). Conclusion: 1. the use of DNFB sensitization and stimulation of mouse skin surface can successfully establish a mouse atopic dermatitis model.2.TSLP in the skin tissue and serum of atopic dermatitis mice, TSLP can promote the expression of IL-4 and IgE, and inhibit the expression of IFN- gamma, and it is in atopic dermatitis. The pathogenesis of.3. atopic dermatitis may play an important role in the pathogenesis of atopic dermatitis, which may play an important role in the serum levels of IL-4, IFN- gamma and IgE in the serum of mice with atopic dermatitis.
【學(xué)位授予單位】：瀘州醫(yī)學(xué)院
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2012
【分類號(hào)】：R758.2;R-332

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