本文選題：梅毒 + 紅細(xì)胞��； 參考：《蘭州大學(xué)》2010年碩士論文

【摘要】： 梅毒是由梅毒螺旋體引起的一種危害性極大的慢性傳染病,主要通過(guò)性接觸和血液傳播。梅毒的發(fā)病機(jī)制未完全闡明,出現(xiàn)血清固定的確切原因目前尚不清楚。當(dāng)前對(duì)梅毒發(fā)病機(jī)制和血清固定原因的研究主要集中在白細(xì)胞免疫,認(rèn)為梅毒發(fā)病與T細(xì)胞介導(dǎo)的免疫反應(yīng)密切相關(guān)。近年來(lái)紅細(xì)胞免疫逐漸成為感染性疾病的研究熱點(diǎn)之一,有研究表明梅毒感染者的紅細(xì)胞免疫功能較正常人明顯降低,但關(guān)于梅毒患者CR1密度相關(guān)基因多態(tài)性的研究尚未見(jiàn)報(bào)道。 目的探討梅毒患者紅細(xì)胞CR1密度相關(guān)基因型的頻率分布。 方法采用聚合酶鏈反應(yīng)(PCR)、限制性內(nèi)切酶HindⅢ酶切技術(shù)測(cè)定患者紅細(xì)胞CR1密度相關(guān)基因多態(tài)性。 結(jié)果病例組中CR1基因HH、HL和LL基因型分布頻率分別為58.75%、35%、和6.25%,對(duì)照組中HH、HL和LL基因型分布頻率分別為68.75%、26.25%和5%,兩組CR1基因型的分布頻率差異無(wú)顯著性(p0.05)；梅毒患者組基因突變率為41.25%,對(duì)照組為31.25%,也無(wú)顯著性差異(p0.05)。 結(jié)論梅毒患者的紅細(xì)胞免疫功能低下與紅細(xì)胞CR1基因發(fā)生突變關(guān)系不密切。
[Abstract]:Syphilis is a serious chronic infectious disease caused by Treponema pallidum, mainly through sexual contact and blood transmission. The pathogenesis of syphilis is not fully clarified, and the exact cause of serum fixation is unclear. At present, the pathogenesis of syphilis and the cause of serum fixation are mainly focused on leukocyte immunity. It is believed that the pathogenesis of syphilis is closely related to T cell mediated immune response. In recent years, red blood cell immunity has gradually become one of the hotspots in infectious diseases. Some studies have shown that the red cell immune function of syphilis infected people is significantly lower than that of normal people. However, the study of CR1 density related gene polymorphism in syphilis patients has not been reported. Objective to investigate the frequency distribution of erythrocyte CR1 density related genotypes in syphilis patients. Methods Polymerase chain reaction (PCR) and restriction endonuclease (Hind 鈪，

本文編號(hào)：1819477