本文關(guān)鍵詞： 瘢痕癌 PIK/AKT信號傳導(dǎo)通路 Bcl- 凋亡　出處：《中華腫瘤防治雜志》2014年08期 　論文類型：期刊論文

【摘要】：目的:探討PI3K/AKT信號傳導(dǎo)通路中PI3K、AKT及其下游靶基因編碼的Bcl-2蛋白與瘢痕癌癌細(xì)胞凋亡的相關(guān)性。方法:以15例病理性皮膚瘢痕被覆上皮和20例皮膚瘢痕癌組織為研究對象,以10例正常皮膚表皮組織為對照。這些標(biāo)本均為石蠟包埋組織,源于遵義醫(yī)學(xué)院附屬第一和第五醫(yī)院病理科2006-01-01-2011-12-30的外檢標(biāo)本。1)采用免疫組織化學(xué)技術(shù)(SP法)分別檢測以上3組組織中PI3K、AKT和Bcl-2蛋白的表達(dá)。2)采用核酸分子原位雜交技術(shù)檢測PI3K mRNA、AKT mRNA的表達(dá)。3)以原位末端標(biāo)記法(Tunel法)檢測細(xì)胞的凋亡水平。4)免疫組化和原位雜交圖像運(yùn)用圖像分析軟件計算其平均光密度和陽性面積,Tunel圖像計算其凋亡指數(shù),所得數(shù)據(jù)運(yùn)用SPSS 16.0軟件包進(jìn)行統(tǒng)計學(xué)分析。結(jié)果:1)PI3K蛋白和PI3K mRNA在正常皮膚表皮、病理性瘢痕上皮和瘢痕癌組織中分別呈陰性、弱陽性和陽性表達(dá)。瘢痕癌組PI3K表達(dá)水平為0.172±0.039,表達(dá)強(qiáng)度為0.301±0.045,與正常皮膚組0.004±0.003和0.185±0.021,病理性瘢痕組0.011±0.009和0.203±0.034比較,差異有統(tǒng)計學(xué)意義,P0.01;瘢痕癌組PI3KmRNA表達(dá)水平為0.137±0.037,表達(dá)強(qiáng)度為0.379±0.054,與正常皮膚組0.008±0.007和0.265±0.016,病理性瘢痕組0.027±0.012和0.293±0.031比較,差異有統(tǒng)計學(xué)意義,P0.01;但正常皮膚組與病理性瘢痕組比較,差異無統(tǒng)計學(xué)意義,P0.05。2)AKT蛋白在瘢痕癌組表達(dá)水平為0.168±0.052,表達(dá)強(qiáng)度為0.388±0.081,與正常皮膚組0.005±0.005和0.218±0.036,病理性瘢痕組0.028±0.025和0.282±0.049比較,差異有統(tǒng)計學(xué)意義,P0.01;AKT mRNA在瘢痕癌組表達(dá)水平為0.144±0.032,表達(dá)強(qiáng)度為0.345±0.031,與正常皮膚組0.017±0.004和0.244±0.025,病理性瘢痕組0.037±0.019和0.257±0.027比較,差異有統(tǒng)計學(xué)意義,P0.01;正常皮膚組與病理性瘢痕組比較,差異無統(tǒng)計學(xué)意義,P0.05。3)Bcl-2在3組組織中均呈陰性表達(dá)。其表達(dá)水平、表達(dá)強(qiáng)度在正常皮膚組(0.006±0.003和0.168±0.019)、病理性瘢痕組(0.008±0.005和0.175±0.027)和瘢痕癌組(0和0.167±0.015)之間比較,差異無統(tǒng)計學(xué)意義,P=0.136。4)正常皮膚組和病理性瘢痕組AI分別為(8.48±0.78)%和(8.14±0.53)%,高于瘢痕癌組(6.41±0.60)%,且差異有統(tǒng)計學(xué)意義,P=0.01;但正常皮膚組與病理性皮膚瘢痕組比較,差異無統(tǒng)計學(xué)意義,P=0.129。結(jié)論:PI3K/AKT信號傳導(dǎo)通路的抗凋亡途徑,可能是通過Bcl-2以外的其他效應(yīng)分子來實現(xiàn)的。
[Abstract]:Objective: to investigate the relationship between the apoptosis of scar cancer cells and the Bcl-2 protein encoded by PI3KnakT and its downstream gene in PI3K/AKT signal transduction pathway. Methods: 15 cases of pathological skin scar coated epithelium and 20 cases of skin scar carcinoma tissue were studied. Ten normal skin epidermis tissues were used as controls. Samples from the Department of Pathology of the first affiliated Hospital of Zunyi Medical College and the Department of Pathology of 5th Hospital, from January to 30, 2006 01-01-2011-12-30. (1) Immunohistochemical technique was used to detect the expression of PI3KFAKT and Bcl-2 protein in the tissues of the above three groups. 2) Nucleic acid in situ hybridization was used. The expression of PI3K mRNA-AKT mRNA was detected by using in situ end labeling (Tunel method). (4) Immunohistochemistry and in situ hybridization images were used to calculate the average optical density and the positive area of apoptotic index. The data were analyzed by SPSS 16.0 software package. Results the protein of PI3K and PI3K mRNA were negative in normal skin epidermis, pathological scar epithelium and scar carcinoma, respectively. The expression level of PI3K was 0.172 鹵0.039 and 0.301 鹵0.045 in scar carcinoma group, which was compared with that in normal skin group (0.004 鹵0.003) and 0.185 鹵0.021, pathological scar group (0.011 鹵0.009) and pathological scar group (0.203 鹵0.034). The expression level of PI3KmRNA in scar cancer group was 0.137 鹵0.037 and 0.379 鹵0.054, which was significantly higher than that in normal skin group (0.008 鹵0.007 and 0.265 鹵0.016), pathological scar group (0.027 鹵0.012) and pathological scar group (0.293 鹵0.031), but there was significant difference between normal skin group and pathological scar group. There was no significant difference in the expression level of P0.05.2AKT protein in the scar carcinoma group (0.168 鹵0.052), and the expression intensity was 0.388 鹵0.081, which was significantly higher than that in the normal skin group (0.005 鹵0.005) and 0.218 鹵0.036 (P < 0.05), and in the pathological scar group (0.028 鹵0.025) and 0.282 鹵0.049 (P < 0.05). The expression level of P0.01AKT mRNA was 0.144 鹵0.032 and 0.345 鹵0.031 in scar carcinoma group, which was significantly higher than that in normal skin group (0.017 鹵0.004) and pathological scar group (0.257 鹵0.027). There was no significant difference in the expression of P0.05.3Bcl 2 in the three groups, and the expression level of P0.05.3in normal skin group was 0.006 鹵0.003 and 0.168 鹵0.019 in normal skin group, 0.008 鹵0.005 and 0.175 鹵0.027 in pathological scar group, and 0 and 0.167 鹵0.015 in scar carcinoma group. The AI of normal skin group and pathological scar group were 8.48 鹵0.78% and 8.14 鹵0.53, respectively, which were higher than that of scar carcinoma group (6.41 鹵0.60), and the difference was statistically significant (P < 0.01), but the AI of normal skin group and pathological scar group was higher than that of pathological skin scar group. Conclusion the anti-apoptotic pathway of the 1: PI3K / AKT signaling pathway may be achieved by other effector molecules other than Bcl-2.
【作者單位】： 遵義醫(yī)學(xué)院珠海校區(qū)病理學(xué)教研室;遵義醫(yī)學(xué)院珠海校區(qū)臨床醫(yī)療系;遵義醫(yī)學(xué)院附屬第五醫(yī)院病理科;遵義醫(yī)學(xué)院附屬第一醫(yī)院病理科;
【基金】：貴州省科技攻關(guān)項目(2010-3080)
【分類號】：R739.5

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