本文關(guān)鍵詞： 血管瘤 血管畸形 Glut-1 VEGF Ki-67　出處：《新疆醫(yī)科大學(xué)》2012年碩士論文　論文類型：學(xué)位論文

【摘要】：目的：檢測葡萄糖轉(zhuǎn)運(yùn)蛋白-1（glucose transporter-1，Glut-1）、血管內(nèi)皮生長因子（Vascular Endothelium Growth Factor, VEGF）、Ki-67在增殖期嬰兒型血管瘤和血管畸形中的表達(dá)并探討其意義。方法：選取新疆醫(yī)科大學(xué)附屬腫瘤醫(yī)院2006年3月至2011年12月手術(shù)切除并經(jīng)病理證實(shí)的增殖期嬰兒型血管瘤和皮下血管畸形蠟塊標(biāo)本，常規(guī)石蠟切片蘇木精-伊紅染色法（hematoxylin-eosin staining，HE）染色，進(jìn)行病理復(fù)檢分組，其中增殖期血管瘤22例，年齡1月-12月，中位年齡6個月。皮下血管畸形50例，年齡10-41歲，中位年齡27歲。另取25例病理證實(shí)的皮膚雀斑樣痣擴(kuò)切標(biāo)本周圍正常血管做陰性對照，年齡12-37歲，中位年齡24歲。所有標(biāo)本做厚度4μm連續(xù)切片，，常規(guī)脫蠟及水化，采用免疫組織化學(xué)（Immunohistochemistry，IHC）鏈霉菌抗生物素蛋白-過氧化物酶連結(jié)法（streptavidin-perosidase，SP）處理，高溫高壓修復(fù)抗原，每張切片于400倍鏡下，挑取10個視野，每個視野計數(shù)100個血管內(nèi)皮細(xì)胞，1000個細(xì)胞中含陽性細(xì)胞數(shù)，換算成陽性百分?jǐn)?shù)，即陽性表達(dá)率，表達(dá)強(qiáng)度用+表示，+越多強(qiáng)度越強(qiáng)。淡黃色為弱陽性+，棕黃色為中陽性++，棕褐色為強(qiáng)陽性+++。采用獨(dú)立樣本X2檢驗(yàn)對數(shù)據(jù)進(jìn)行分析。結(jié)果：①Glut-1免疫組織化學(xué)：增殖期血管瘤組中的陽性率95.45%，皮下血管畸形及正常血管沒有Glut-1表達(dá)。②VEGF免疫組織化學(xué)：增殖期血管瘤組中的陽性率86.36%，皮下血管畸形28.00%，正常血管24.00%。VEGF在增殖期血管瘤組中的表達(dá)率明顯高于皮下血管畸形及正常血管，差異有極顯著性（P0.01），皮下血管畸形與正常血管表達(dá)沒有差別（P0.05）。③Ki-67免疫組織化學(xué)：增殖期血管瘤陽性率90.91%；皮下血管畸形組和正常血管組各有一例出現(xiàn)Ki-67陽性表達(dá)，陽性率分別為2.00%和4.00%。Ki-67在增殖期血管瘤組中的表達(dá)率明顯高于皮下血管畸形及正常血管，差異有極顯著性（P0.01），皮下血管畸形及正常血管表達(dá)沒有差別（P0.05）。結(jié)論：Glut-1、VEGF和Ki-67的異常表達(dá)與血管瘤的發(fā)生、發(fā)展有一定的關(guān)系，Glut-1、VEGF和Ki-67可以作為血管瘤與血管畸形鑒別診斷的免疫標(biāo)志物，特別是Glut-1與Ki-67可能具有更高的靈敏度和特異度。
[Abstract]:Objective: to detect glucose transporter-1 (Glut-1). Vascular Endothelium Growth Factor (VEGF). Expression and significance of Ki-67 in proliferative infantile hemangioma and vascular malformation. Methods:. Wax samples of proliferative infantile hemangioma and subcutaneous vascular malformation were selected from tumor Hospital affiliated to Xinjiang Medical University from March 2006 to December 2011. Hematoxylin-eosin stenting was stained by hematoxylin-eosin staining in paraffin sections. 22 cases of proliferative hemangioma were divided into two groups. The median age was 6 months from January to December. 50 cases of subcutaneous vascular malformation aged 10-41 years. The median age was 27 years. Another 25 skin freckle nevus specimens, aged 12-37 years, were taken as negative control. The median age was 24 years old. All the specimens were made 4 渭 m thick serial sections, conventional dewaxing and hydration, immunohistochemistry was used. Streptavidin-perosidase SP) was treated with Streptavidin-perosidase (SPN), and the antigen was repaired by high temperature and high pressure. Each section was selected under 400 times microscope, 10 visual fields were selected, 100 vascular endothelial cells were counted in each field, and 1 000 cells contained positive cells, which was converted into positive percentage, that is, positive expression rate. The more the intensity, the stronger the intensity. The light yellow is weak positive, the brown yellow is medium positive. Brown was strongly positive. The data were analyzed by X2 test of independent sample. Results: the positive rate was 95.45% in proliferative hemangioma group. The positive rate of Glut-1 in proliferative hemangioma group was 86.36 and that in subcutaneous vascular malformation was 28.00%. The expression rate of VEGF in proliferative hemangioma group was significantly higher than that in subcutaneous vascular malformation and normal vessels (P 0.01). There was no difference between subcutaneous vascular malformation and normal vascular expression. P0.05 and 3Ki-67 immunohistochemical staining showed that the positive rate of proliferative hemangioma was 90.91%. Positive expression of Ki-67 was found in one case in subcutaneous vascular malformation group and one case in normal vascular group. The positive rates of Ki-67 in proliferative hemangioma group were significantly higher than those in subcutaneous vascular malformation and normal vessels (P 0.01). There was no difference between subcutaneous vascular malformation and normal vascular expression (P 0.05). Conclusion the abnormal expression of Ki-67 and Ki-67 may be related to the occurrence and development of hemangioma. Glut-1VEGF and Ki-67 can be used as immune markers for differential diagnosis of hemangioma and vascular malformation, especially Glut-1 and Ki-67 may have higher sensitivity and specificity.
【學(xué)位授予單位】：新疆醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2012
【分類號】：R739.5

【共引文獻(xiàn)】

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1 夏章權(quán);張從紀(jì);王濤;;VEGF165/HBD3雙基因共表達(dá)真核質(zhì)粒載體的構(gòu)建及其體外表達(dá)[J];第三軍醫(yī)大學(xué)學(xué)報;2011年05期

2 曹亞先;張雪林;;四肢肌間海綿狀血管瘤的MRI診斷[J];放射學(xué)實(shí)踐;2010年07期

3 宋其韜;王林森;;肌肉血管瘤影像學(xué)特征[J];中國骨腫瘤骨病;2006年04期

4 趙紅衛(wèi),肖運(yùn)祥,周納新;肢體肌肉海綿狀血管瘤的診斷及治療[J];湖北三峽學(xué)院學(xué)報;2000年S1期

5 邱璇;賀聲;張?jiān)粕?路平;;肌間血管瘤的彩色多普勒超聲診斷及鑒別診斷[J];海軍醫(yī)學(xué)雜志;2011年05期

6 梁宇闖;李卓永;林堅(jiān);龍晚生;李宗業(yè);麥栩榆;;介入栓塞治療四肢肌間血管瘤的遠(yuǎn)期療效[J];中國介入影像與治療學(xué);2007年05期

7 孫天駿;韓焱福;柴家科;;VEGF基因治療與血管化作用的研究進(jìn)展[J];中國美容醫(yī)學(xué);2010年02期

8 李岳令;陳石海;劉柳;段紅鈺;;不同濃度乙醇對大鼠肌肉組織影響的探討[J];中國美容醫(yī)學(xué);2011年02期

9 劉學(xué)鍵;徐珞;史斌;秦中平;邰茂眾;李克雷;孟凡順;;四肢骨骼肌血管畸形的分類與治療方法的研究[J];中國現(xiàn)代普通外科進(jìn)展;2007年01期

10 蔣易;沈楨巍;雷撼;馬少林;白建文;;VEGF對大鼠腦缺血再灌注損傷的預(yù)防作用及其機(jī)制研究[J];現(xiàn)代生物醫(yī)學(xué)進(jìn)展;2012年27期

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