發(fā)布時(shí)間：2019-07-27 07:55

【摘要】：目的檢測(cè)PKCzeta在不同分級(jí)前列腺癌(PCa)組織的表達(dá)情況,并探究PKCzeta的表達(dá)對(duì)前列腺癌細(xì)胞系增殖的影響。方法采用免疫組織化學(xué)染色法檢測(cè)19例前列腺癌組織和4例正常前列腺組織的PKCzeta表達(dá)情況,并按腫瘤分級(jí)分為3組,進(jìn)行PKCzeta表達(dá)的差異性分析;應(yīng)用Hilymax試劑轉(zhuǎn)染PKCzeta質(zhì)粒(CA-PKCzeta)過(guò)表達(dá)前列腺癌細(xì)胞PKCzeta;通過(guò)實(shí)時(shí)熒光定量PCR(RT-qPCR)和Western blot分別檢測(cè)PC3和DU145細(xì)胞內(nèi)PKCzeta的mRNA和蛋白水平;利用平板克隆形成試驗(yàn)和CCK-8試劑盒檢測(cè)PC3和DU145細(xì)胞增殖能力的差異以及轉(zhuǎn)染PKCzeta質(zhì)粒后細(xì)胞增殖的變化。結(jié)果 PKCzeta在前列腺癌組織中的表達(dá)高于正常前列腺組織(P0.05),而且低分級(jí)(1級(jí))前列腺癌PKCzeta的表達(dá)高于高分級(jí)(2~3級(jí))前列腺癌(P0.05)。DU145細(xì)胞的PKCzeta表達(dá)高于PC3細(xì)胞,而其增殖速度較PC3細(xì)胞低;過(guò)表達(dá)PKCzeta使前列腺癌細(xì)胞系的增殖受抑。結(jié)論 PKCzeta與腫瘤分級(jí)存在負(fù)相關(guān)關(guān)系,在癌癥早期可能有一定的保護(hù)作用,且過(guò)表達(dá)PKCzeta能抑制前列腺癌細(xì)胞的增殖。
[Abstract]:Objective to detect the expression of PKCzeta in different grades of prostate cancer (PCa) and to explore the effect of PKCzeta expression on the proliferation of prostate cancer cell line. Methods Immunohistochemical staining was used to detect the expression of PKCzeta in 19 cases of prostate cancer and 4 cases of normal prostate tissue, and the expression of PKCzeta was divided into three groups according to tumor grade, and the mRNA and protein levels of PKCzeta in PC3 and DU145 cells were detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot respectively. Plate clone formation test and CCK-8 kit were used to detect the difference of proliferation ability between PC3 and DU145 cells and the change of cell proliferation after PKCzeta plasmid transfer. Results the expression of PKCzeta in prostate cancer was higher than that in normal prostate (P 0.05), and the expression of PKCzeta in low grade (grade 1) prostate cancer was higher than that in high grade (2 鈮，

本文編號(hào)：2519865