發(fā)布時間：2019-07-27 07:55

【摘要】：目的檢測PKCzeta在不同分級前列腺癌(PCa)組織的表達情況,并探究PKCzeta的表達對前列腺癌細胞系增殖的影響。方法采用免疫組織化學染色法檢測19例前列腺癌組織和4例正常前列腺組織的PKCzeta表達情況,并按腫瘤分級分為3組,進行PKCzeta表達的差異性分析;應用Hilymax試劑轉(zhuǎn)染PKCzeta質(zhì)粒(CA-PKCzeta)過表達前列腺癌細胞PKCzeta;通過實時熒光定量PCR(RT-qPCR)和Western blot分別檢測PC3和DU145細胞內(nèi)PKCzeta的mRNA和蛋白水平;利用平板克隆形成試驗和CCK-8試劑盒檢測PC3和DU145細胞增殖能力的差異以及轉(zhuǎn)染PKCzeta質(zhì)粒后細胞增殖的變化。結(jié)果 PKCzeta在前列腺癌組織中的表達高于正常前列腺組織(P0.05),而且低分級(1級)前列腺癌PKCzeta的表達高于高分級(2~3級)前列腺癌(P0.05)。DU145細胞的PKCzeta表達高于PC3細胞,而其增殖速度較PC3細胞低;過表達PKCzeta使前列腺癌細胞系的增殖受抑。結(jié)論 PKCzeta與腫瘤分級存在負相關(guān)關(guān)系,在癌癥早期可能有一定的保護作用,且過表達PKCzeta能抑制前列腺癌細胞的增殖。
[Abstract]:Objective to detect the expression of PKCzeta in different grades of prostate cancer (PCa) and to explore the effect of PKCzeta expression on the proliferation of prostate cancer cell line. Methods Immunohistochemical staining was used to detect the expression of PKCzeta in 19 cases of prostate cancer and 4 cases of normal prostate tissue, and the expression of PKCzeta was divided into three groups according to tumor grade, and the mRNA and protein levels of PKCzeta in PC3 and DU145 cells were detected by real-time fluorescence quantitative PCR (RT-qPCR) and Western blot respectively. Plate clone formation test and CCK-8 kit were used to detect the difference of proliferation ability between PC3 and DU145 cells and the change of cell proliferation after PKCzeta plasmid transfer. Results the expression of PKCzeta in prostate cancer was higher than that in normal prostate (P 0.05), and the expression of PKCzeta in low grade (grade 1) prostate cancer was higher than that in high grade (2 鈮，

本文編號：2519865