【摘要】：目的:探討脂多糖對(duì)膀胱癌細(xì)胞株Cx43和TLR4蛋白的表達(dá),及其對(duì)細(xì)胞增殖和凋亡的影響。方法:體外培養(yǎng)膀胱癌5637細(xì)胞,在LPS刺激下,采用蛋白質(zhì)印跡法檢測(cè)膀胱癌5637細(xì)胞中TLR4和Cx43蛋白的表達(dá),通過(guò)CCK-8檢測(cè)5637-control組和5637-LPS組細(xì)胞的增殖情況;利用流式細(xì)胞術(shù)檢測(cè)5637-control組、5637-LPS組和5637-LPS+順鉑組細(xì)胞的凋亡情況。結(jié)果:在LPS刺激下,膀胱癌5637細(xì)胞中的Cx43基因表達(dá)較對(duì)照組明顯增高,兩組之間比較差異有統(tǒng)計(jì)學(xué)意義(P=0.012),而5637-LPS組(LPS,2μg/mL,24 h)中TLR4及Cx43的表達(dá)均較5637-control組明顯增高,差異有統(tǒng)計(jì)學(xué)意義(P=0.019,P=0.003)。CCK-8結(jié)果顯示,在LPS刺激下,5637-LPS組隨著時(shí)間(0、1、2、3 d)的增加,細(xì)胞的增殖較5637-control組明顯,差異有統(tǒng)計(jì)學(xué)意義(P=0.018)。流式細(xì)胞儀檢測(cè)結(jié)果顯示,5637-control組和5637-LPS組凋亡率分別為(8.3±1.58)%和(7.8±2.03)%,差異無(wú)統(tǒng)計(jì)學(xué)意義(P=0.099)。而5637-順鉑組凋亡率[(60±4.35)%]較5637-順鉑+LPS組[(52±6.25)%]高,差異有統(tǒng)計(jì)學(xué)意義(P=0.019)。結(jié)論:在LPS刺激下,膀胱腫瘤細(xì)胞可能通過(guò)提高TLR4和Cx43的表達(dá)來(lái)介導(dǎo)腫瘤細(xì)胞逃脫免疫監(jiān)視。
[Abstract]:Aim: to investigate the expression of lipopolysaccharide (lipopolysaccharide) on bladder cancer cell line Cx43 and TLR4, and the effect of lipopolysaccharide on cell proliferation and apoptosis. Methods: bladder cancer cell line 5637 was cultured in vitro. The expression of TLR4 and Cx43 protein in bladder cancer 5637 cell line was detected by Western imprinting, the proliferation of 5637-control group and 5637-LPS group was detected by CCK-8, and the apoptosis of 5637-control group, 5637-LPS group and 5637-LPS platinum group was detected by flow cytometry. Results: the expression of Cx43 gene in bladder cancer 5637 cells stimulated by LPS was significantly higher than that in the control group (P 鈮，

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