【摘要】：目的：探討解脲脲原體(Ureaplasma urealytium, Uu)血清型1(Uu1)和4(Uu4)在雌性小鼠生殖道中的致病性。 方法：將408只雌激素預處理過的小鼠隨機分為Uu1組和Uu4組,實驗組小鼠陰道分別接種三種濃度不同的Uu標準株菌液。對照組小鼠接種無菌培養(yǎng)基。接種后分別于第1、3、7、14、28、42天隨機取實驗組10只、對照組4只將其處死后取小鼠陰道及宮頸分泌物做熒光定量聚合酶連反應(FQ-PCR)檢測解脲脲原體的濃度,并取部分子宮體、輸卵管、卵巢組織置于液體培養(yǎng)基檢測Uu。取陰道、宮頸、子宮及輸卵管、卵巢組織行HE染色進行組織病理學觀察。 結果：(1)陰道和宮頸分泌物：Uu1及Uu4三組之間總陽性率的差異均有統計學意義(P0.01)。Uu1和Uu4組同種濃度三組之間總陽性率的差異均無統計學意義(P0.05)(2)至觀測結束時,Uu1及Uu4三組之間小鼠陰道及宮頸分泌物Uu濃度,各組各時間點濃度比較差異均有統計學意義(P0.05)。(3)Uu組織培養(yǎng)顯示Uu1、Uu4組子宮體、輸卵管Uu培養(yǎng)陽性率差異均有統計學意義(P0.05)。(4)接種后3-42d,Uu1、Uu4組宮頸總致病率的差異有統計學意義(P0.05)。接種后7-42d, Uu1、Uu4組子宮體與輸卵管致病率的差異均有統計學意義(P0.05)。 結論：(1)Uu1和Uu4主要在宮頸致病,部分上行可導致上生殖道感染,在陰道可致短期炎癥。(2)Uu4的在宮頸和子宮、輸卵管的致病性比Uu1強且致病力隨Uu感染的濃度增大而增強。
[Abstract]:Objective: to investigate the pathogenicity of Ureaplasma Urealyticum (Ureaplasma urealytium, Uu) serotypes 1 (Uu1) and 4 (Uu4) in female mice reproductive tract. Methods: 408 mice pretreated with estrogen were randomly divided into Uu1 group and Uu4 group. The mice in the experimental group were inoculated with three kinds of Uu standard strains of different concentrations in vagina. The control group mice were inoculated with aseptic medium. After inoculation, 10 mice in the experimental group were randomly selected on the 1st day after inoculation, and 4 mice in the control group were killed. The vaginal and cervical secretions of the mice were taken for fluorescence quantitative polymerase chain reaction (FQ-PCR) to detect the concentration of Ureaplasma Urealyticum, and some uterine bodies and fallopian tubes were taken out. Detection of Uu. in Ovarian tissue on liquid medium The tissues of vagina, cervix, uterus and oviduct were stained with HE for histopathological observation. Results: (1) vaginal and cervical secretions: there was significant difference in total positive rate between Uu1 and Uu4 groups (P0.01). There was no significant difference in total positive rate between Uu1 and Uu4 groups at the same concentration (P0.05) (2), Uu1 and Uu4 at the end of observation. The concentration of Uu in vaginal and cervical secretions of mice between the three groups, There were significant differences in the concentration at each time point in each group (P0.05). (3) Uu tissue culture showed that the positive rate of uterine body and tubal Uu culture in Uu1,Uu4 group were significantly different (P0.05). (4) the total pathogenicity of cervix of 3 ~ 42 days UU1 UU4 group was significantly different (P0.05). From 7 to 42 days after inoculation, the pathogenicity of uterine body and fallopian tube in Uu1,Uu4 group were significantly different (P0.05). Conclusion: (1) Uu1 and Uu4 are mainly pathogenic in the cervix, partly uplink can lead to upper genital tract infection, and in vagina can cause short-term inflammation. (2) the pathogenicity of Uu4 in cervix and uterus is stronger than that of Uu1, and the pathogenicity increases with the increase of Uu infection concentration.
【學位授予單位】：青島大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R691.3

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