【摘要】：目的建立一種腎功能無異常的三聚氰胺腎結(jié)石模型，探討腎茶及迷迭香酸對泌尿生殖系統(tǒng)可能的保護(hù)作用。 方法采用兩種造模方法實驗：①將50只ICR雄性小鼠隨機(jī)分為正常組、模型組（隔天染毒）、迷迭香酸低劑量組、迷迭香酸高劑量組、友來特組（隔天染毒），上午正常組予NS，其余各組予Mel+Cya，下午各組分別予NS、NS、低劑量RA、高劑量RA、友來特，隔天染毒，30天后處死；②將40只ICR雄性小鼠隨機(jī)分為模型組（連續(xù)染毒）、迷迭香酸組、腎茶組、友來特組（連續(xù)染毒），上午各組予Mel+Cya，，下午分別予NS、RA、腎茶、友來特，連續(xù)灌胃15天。評價各組小鼠的一般情況、體質(zhì)量、飲水量，檢測血清Cr、BUN、UA、ALT、AST、MDA、SOD，觀察腎臟和睪丸的病變，檢測腎臟中三聚氰胺的含量。 結(jié)果隔天染毒30天后，各組小鼠體質(zhì)量、血生化和氧化指標(biāo)統(tǒng)計學(xué)無差異（P＞0.05）；腎臟病理：模型組（隔天染毒）腎小管腔內(nèi)可見中等量黃色物質(zhì)沉積，偶見結(jié)晶形成，友來特組（隔天染毒）腎小管腔內(nèi)未見明顯沉積物；睪丸病理：模型組（隔天染毒）睪丸精子細(xì)胞和成熟精子數(shù)目明顯減少，友來特組（隔天染毒）基本正常；腎臟三聚氰胺含量比較：迷迭香酸低劑量組＞模型組（隔天染毒）＞迷迭香酸高劑量組＞友來特組（隔天染毒）＞正常對照組。Mel+Cya連續(xù)染毒15天，腎茶組和友來特組（連續(xù)染毒）小鼠體重增長較快、飲水量較多；各組小鼠肝腎功能正常，統(tǒng)計學(xué)無差異（P＞0.05）；腎臟病理：模型組（連續(xù)染毒）腎小管腔內(nèi)可見大量晶體聚集，腎茶治療組腎小管內(nèi)偶見少許沉積物，未見結(jié)晶形成，友來特組（連續(xù)染毒）腎小管內(nèi)可見沉積物，偶見結(jié)晶；睪丸病理：模型組（連續(xù)染毒）睪丸生精細(xì)胞排列疏松，成熟精子和間質(zhì)細(xì)胞減少，腎茶治療組和友來特組（連續(xù)染毒）小鼠睪丸組織形態(tài)完整；各組腎臟三聚氰胺含量比較：迷迭香酸組＞模型組（連續(xù)染毒）＞友來特組（連續(xù)染毒）＞腎茶治療組。 結(jié)論腎茶對三聚氰胺導(dǎo)致的腎結(jié)石具有明顯的防治作用；同時能夠減輕三聚氰胺及其同系物對睪丸精子生長的抑制作用；迷迭香酸可能會減輕三聚氰胺導(dǎo)致的腎損傷。
[Abstract]:Objective to establish a melamine nephrolithiasis model without abnormal renal function and to explore the protective effect of kidney tea and rosemary acid on urinary and reproductive system. Methods two kinds of modeling methods were used: (1) 50 male ICR mice were randomly divided into normal group, model group, rosemary acid low dose group and rosemary acid high dose group. Eurite group (next day) and normal group (morning group) were given Mel Cya, for the rest of the morning. In the afternoon, each group was given NS, low dose RA, high dose RA,. The next day, the mice were killed 30 days later. 2Forty ICR male mice were randomly divided into three groups: model group (continuous exposure), rosemary acid group, kidney tea group and eurite group (continuous exposure). In the morning, each group was given NS,RA, kidney tea in the afternoon, eurite, continuous intragastric administration for 15 days. The general condition, body weight, drinking quantity, serum Cr,BUN,UA,ALT,AST,MDA,SOD, and the contents of melamine in kidney and testis were evaluated. Results 30 days after exposure, there was no significant difference in body weight, blood biochemistry and oxidation index in each group (P > 0.05). Testicular pathology: the number of testicular spermatozoa and mature spermatozoa decreased significantly in the model group (the next day), and the number of spermatozoa in the eurite group (exposed to poison the next day) was basically normal. Comparison of renal melamine content: rosemary acid low dose group > model group (next day exposure) > rosemary acid high dose group > eurite group (next day exposure) > normal control group. Mel Cya exposure for 15 days. The weight of mice in the kidney tea group and the eurite group (continuous exposure) increased rapidly, and the mice drank more water, the liver and kidney function of each group were normal, there was no statistical difference between the two groups (P > 0.05), and there was no significant difference between the two groups (P > 0.05). Renal pathology: in the model group (continuous exposure), a large number of crystal aggregation could be seen in the renal tubule lumen, a little sediment in the renal tubule was occasionally seen in the kidney tea treatment group, and no crystallization was found in the renal tubules, while in the eurite group (continuous exposure), the deposits in the renal tubules were visible and the crystals were occasionally seen. Testicular pathology: testicular spermatogenic cells were loosely arranged, mature spermatozoa and interstitial cells were decreased in model group (continuous exposure), and testicular tissue was intact in kidney tea treatment group and eurite group (continuous exposure group). Comparison of the contents of melamine in kidney: rosmarinic acid group > model group (continuous exposure) > eurite group (continuous exposure) > kidney tea treatment group. Conclusion Shencha has obvious preventive and therapeutic effects on renal stones induced by melamine and can reduce the inhibitory effect of melamine and its congeners on testicular spermatozoa growth. Rosmarinic acid may alleviate renal injury induced by melamine.
【學(xué)位授予單位】：福建醫(yī)科大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R692.4

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