【摘要】：目的:觀察紅細(xì)胞生成素(erythropoietin,EPO)對(duì)過(guò)氧化氫(H_2O_2)刺激后紅細(xì)胞衰亡(eryptosis)和紅細(xì)胞中活性氧簇(reactive oxygen species,ROS)生成的影響,并探討其可能機(jī)制。方法:將1%健康人紅細(xì)胞懸液在以下3組不同的體外培養(yǎng)液中孵育:對(duì)照組(C組,培養(yǎng)基為PBS液)、H_2O_2組(H組,培養(yǎng)基為H_2O_2終濃度100μmol/L的PBS液)和EPO組(E組,培養(yǎng)基為H_2O_2終濃度100μmol/L、EPO終濃度2×10~4U/L的PBS液)。分別在孵育24 h和60 h時(shí),留取紅細(xì)胞以備檢測(cè)。使用流式細(xì)胞術(shù)檢測(cè)紅細(xì)胞的衰亡率、紅細(xì)胞內(nèi)ROS和紅細(xì)胞內(nèi)鈣離子濃度(_i~([Ca2+])),觀察各檢測(cè)指標(biāo)的變化并分析其相關(guān)性。結(jié)果:紅細(xì)胞衰亡率在C組隨孵育時(shí)間延長(zhǎng)而增加,在相同觀察時(shí)點(diǎn),H組較C組明顯增加(P0.01),E組較H組明顯降低(P0.01)。H組紅細(xì)胞的ROS生成較C組明顯增多,_i~([Ca2+])較C組明顯升高(P0.01);E組紅細(xì)胞的ROS生成較H組明顯減少,_i~([Ca2+])較H組明顯降低(P0.05或P0.01)。結(jié)論:H_2O_2誘導(dǎo)健康紅細(xì)胞加速衰亡,而EPO可以抑制H_2O_2誘導(dǎo)的紅細(xì)胞衰亡,其機(jī)制可能與抗氧化及_i~([Ca2+])的改變有關(guān)。
[Abstract]:Aim: to investigate the effects of erythropoietin (EPO) on the production of (eryptosis) and reactive oxygen species (Ros) in erythrocyte induced by hydrogen peroxide (H_2O_2), and to explore its possible mechanism. Methods: 1% healthy human erythrocyte suspension was incubated in the following three groups in vitro: control group (group C, PBS solution) and group H _ 2O _ 2 (H group, PBS solution with 100 渭 mol/L H_2O_2 final concentration) and EPO group (E group). The medium was PBS solution with final concentration of 100 渭 mol / L H_2O_2 and final concentration of 2 脳 10~4U/L. After incubation for 24 h and 60 h, red blood cells were collected for detection. The decay rate of red blood cells, the concentration of ROS and calcium in red blood cells were detected by flow cytometry. Results: erythrocyte decay rate increased with the increase of incubation time in group C. At the same observation point, the ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocytes in group E was significantly lower than that in group H (P0.01). The ROS production of erythrocytes in group H was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly higher than that in group C (P0.01). The ROS production of erythrocyte in group E was significantly lower than that in group H, and that in group H was significantly lower than that in group H. Group A was significantly lower (P0.05 or P0.01). Conclusion\%\
【作者單位】： 山東大學(xué)第二醫(yī)院腎內(nèi)科;
【基金】：山東大學(xué)第二醫(yī)院種子基金資助項(xiàng)目(No.S2015010001) 山東省自然科學(xué)基金資助項(xiàng)目(No.ZR2014HM045)
【分類號(hào)】：R692.5

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