【摘要】：目的研究microR NA-224(miR-224)對前列腺癌生化復(fù)發(fā)及血管生成的影響。方法生物信息學(xué)分析及熒光素酶報(bào)告實(shí)驗(yàn)預(yù)測細(xì)胞周期調(diào)節(jié)蛋白1(CNNM1)受miR-224負(fù)性調(diào)控。Taylor前列腺癌數(shù)據(jù)庫分析、驗(yàn)證CNNM1、miR-224的表達(dá)關(guān)系及其與前列腺癌生化復(fù)發(fā)的相關(guān)性。前列腺癌PC3細(xì)胞體外培養(yǎng)及動物體內(nèi)成瘤實(shí)驗(yàn)研究CNNM1、miR-224表達(dá)對前列腺癌微血管生成標(biāo)志物CD31的影響。結(jié)果 CNNM1表達(dá)受miR-224靶向調(diào)節(jié),前列腺癌組織中CNNM1與miR-224的表達(dá)呈負(fù)相關(guān)(P0.05)。miR-224與前列腺癌的生化復(fù)發(fā)呈負(fù)相關(guān)(P0.05),CNNM1與前列腺的生化復(fù)發(fā)呈正相關(guān)(P0.05);在過表達(dá)miR-224的PC3細(xì)胞株內(nèi),CNNM1和CD31的表達(dá)量下降;CNNM1過表達(dá)能促進(jìn)CD31的生成。前列腺癌細(xì)胞裸鼠體內(nèi)成瘤組織免疫組織化學(xué)法染色提示,miR-224過表達(dá)能抑制前列腺癌組織內(nèi)微血管形成。結(jié)論 miR-224通過靶向調(diào)控CNNM1表達(dá),抑制前列腺癌微血管形成,控制前列腺癌生化復(fù)發(fā)。
[Abstract]:Objective to study the effect of microR NA-224 (miR-224) on the biochemical recurrence and angiogenesis of prostate cancer. Methods bioinformatics analysis and luciferase report assay were used to predict the relationship between the expression of cell cycle regulator protein 1 (CNNM1) and the biochemical recurrence of prostate cancer. Study on the effect of CNNM1 miR-224 expression on prostate cancer microangiogenesis marker CD31 in vitro and in vivo tumorigenesis of prostate cancer PC3 cells. Results the expression of CNNM1 was regulated by miR-224. There was a negative correlation between the expression of CNNM1 and miR-224 in prostate cancer tissues (P0.05). MiR-224 was negatively correlated with the biochemical recurrence of prostate cancer (P0.05) and CNNM1 was positively correlated with the biochemical recurrence of prostate (P0.05), and the expression of CNNM1 and CD31 decreased in PC3 cell lines that overexpressed miR-224. Danone promotes the formation of CD31. Immunohistochemical staining showed that the overexpression of mmiR-224 could inhibit the formation of microvessel in prostate cancer tissues in nude mice. Conclusion miR-224 can inhibit the microangiogenesis of prostate cancer and control the recurrence of prostate cancer by targeting the expression of CNNM1.
【作者單位】： 中山大學(xué)附屬中山醫(yī)院泌尿外科;
【基金】：中國博士后科學(xué)基金(No:2016M590842) 廣東省醫(yī)學(xué)科研基金(No:A2016052) 廣東省中山市科技計(jì)劃(No:2016B1028)
【分類號】：R737.25
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本文編號：2192814