本文選題：單個核細胞核轉(zhuǎn)錄因子κBp65 + 白介素-17　； 參考：《安徽中醫(yī)藥大學》2014年碩士論文

【摘要】：目的：觀察慢性腎衰竭（chronic renal failure,CRF）濕熱證患者血清白介素-17（interleukin17,IL-17）、外周血單個核細胞（peripheral blood mononuclearcells,PBMC）核轉(zhuǎn)錄因子-κBp65(nuclear factors-κB,NF-κBp65）、血清Ⅲ型膠原（collagen type Ⅲ,Col-Ⅲ）的變化情況及清腎顆粒的干預作用。 方法：根據(jù)隨機數(shù)字表將68例CRF濕熱證患者隨機分為治療組和對照組各34例，并選取30例健康體檢者作為正常組。過程中失訪7例（因加服金水寶剔除者3例，加服百令膠囊剔除者4例），其中治療組4例，對照組3例，實際完成61例，治療組30例，對照組31例。治療組男18例，女12例，年齡平均（53.20±9.41）歲，對照組男19例，女12例，年齡平均（49.06±12.53）歲。治療組與對照組均給予西醫(yī)基礎(chǔ)治療及中藥保留灌腸，治療組加用清腎顆粒，每日3次，每次1袋，療程8周。檢測治療組與對照組治療前后血肌酐（serum creatinine,Scr）、IL-17、Col-Ⅲ及PBMC中NF-κBp65水平，估算腎小球濾過率（estimatedglomerular filtration rate,eGFR），并觀察兩組患者治療前后中醫(yī)癥候積分變化情況。正常組檢測血清IL-17、Col-Ⅲ及外周血PBMC中NF-κBp65的含量。 結(jié)果：1.臨床疾病療效比較：治療組疾病療效總有效率為86.67%，對照組為58.06%，兩組比較差異有統(tǒng)計學意義（P0.05）。2.中醫(yī)證候療效比較：治療組中醫(yī)證候療效總有效率為86.67%，對照組為45.16%，兩組比較有顯著性差異（P0.01）。3.中醫(yī)證候積分比較：治療前治療組中醫(yī)證候積分與對照組比較，差異無統(tǒng)計學意義（P0.05），治療組治療后中醫(yī)證候積分顯著低于治療前（P0.01），對照組治療后中醫(yī)證候積分與治療前比較，差異無統(tǒng)計學意義（P0.05），治療組治療后中醫(yī)證候積分顯著低于同期對照組（P0.01）。4.Scr、eGFR比較：（1）治療前治療組與對照組Scr水平的差異無統(tǒng)計學意義（P0.05），治療組治療后Scr水平較治療前明顯降低（P0.01），對照組治療后Scr水平與治療前比較，差異無統(tǒng)計學意義（P0.05），治療組治療后Scr水平低于同期對照組（P0.05）。（2）治療前治療組與對照組eGFR水平的差異無統(tǒng)計學意義（P0.05），治療組治療后eGFR水平較治療前明顯升高（P0.01），對照組治療后eGFR水平與治療前比較，差異無統(tǒng)計學意義（P0.05），治療組治療后eGFR水平高于同期對照組（P0.05）。5.血清IL-17比較：治療組治療前血清IL-17水平較正常組高，差異有顯著性（P0.01），對照組治療前血清IL-17水平較正常組高，差異有顯著性（P0.01）。但治療組和對照組治療前血清IL-17水平比較，差異無統(tǒng)計學意義（P0.05）。治療組治療后血清IL-17水平較治療前降低（P0.05），對照組治療后血清IL-17水平與治療前相比較，差異無統(tǒng)計學意義（P0.05），治療組治療后血清IL-17水平低于同期對照組（P0.05）。6.PBMC中NF-κBp65比較：治療組治療前PBMC中NF-κBp65含量較正常組高，差異有顯著性（P0.01），對照組治療前PBMC中NF-κBp65含量較正常組高，差異有顯著性（P0.01）。但治療組和對照組治療前PBMC中NF-κBp65含量比較，差異無統(tǒng)計學意義（P0.05）。治療組治療后PBMC中NF-κBp65含量較治療前明顯降低（P0.01），對照組治療后PBMC中NF-κBp65含量與治療前相比較，差異無統(tǒng)計學意義（P0.05），治療組治療后PBMC中NF-κBp65含量顯著低于同期對照組（P0.01）。7.血清Col-Ⅲ比較：治療組、對照組治療前血清Col-Ⅲ水平均較正常組高，差異有顯著性（P0.01），治療組和對照組治療前血清Col-Ⅲ水平比較，差異無統(tǒng)計學意義（P0.05）。治療組治療后血清Col-Ⅲ水平較治療前明顯降低（P0.01），對照組治療后血清Col-Ⅲ水平與治療前相比較，差異無統(tǒng)計學意義（P0.05），治療組治療后血清Col-Ⅲ水平顯著低于同期對照組（P0.01）。 結(jié)論：1.清腎顆�？筛纳艭RF濕熱證患者臨床癥狀、降低Scr、升高eGFR。2.CRF濕熱證患者血清IL-17、Col-Ⅲ及PBMC中NF-κBp65水平均升高。3.清腎顆�？梢越档虲RF濕熱證患者血清IL-17、Col-Ⅲ及PBMC中NF-κBp65水平，說明清腎顆粒在防治RF中有較好的作用。4.治療后安全性指標檢測無異常變化，過程中未出現(xiàn)藥物不良反應(yīng)，說明清腎顆粒安全性良好。 目的：通過檢測單側(cè)輸尿管梗阻（unilateral ureteral obstruction,UUO）所致腎間質(zhì)纖維化（renal interstitial fibrosis,RIF）大鼠外周血中輔助性T淋巴細胞（CD4+T細胞）與抑制性T淋巴細胞（CD8+T細胞）比值、Th17細胞比例及腎臟組織中白介素-17(interleukin-17,IL-17)、核轉(zhuǎn)錄因子κB-p65(nuclear factors-κBp65,NF-κBp65)、Ⅲ型膠原(collagen type Ⅲ,Col-Ⅲ)的表達，觀察UUO大鼠是否存在免疫功能的紊亂、炎癥介質(zhì)的高釋放及清腎顆粒的干預作用。 方法：取60只健康雄性SD大鼠，按隨機區(qū)組法隨機分為5組：清腎顆粒組、百令膠囊組、模型組、假手術(shù)組、正常組，每組12只。各組大鼠實驗前檢測血尿素氮（blood urea nitrogen,BUN）、血肌酐（serum creatinine,Scr），結(jié)果差異無統(tǒng)計學意義（P0.05）。清腎顆粒組、百令膠囊組及模型組均以UUO法制備腎間質(zhì)纖維化大鼠模型，假手術(shù)組以前三組同樣的手術(shù)路徑分離輸尿管但不結(jié)扎。清腎顆粒組以0.6g/100g的清腎顆粒溶于4ml溫水中灌胃，百令膠囊組以0.03g/100g的百令膠囊溶于4ml溫水中灌胃，模型組、假手術(shù)組及正常組均以4ml溫水灌胃。各組大鼠每日灌胃一次，療程為3周。實驗大鼠灌胃結(jié)束后腹主動脈取血并取左側(cè)腎臟，術(shù)前留取各組大鼠24小時尿量。生化法檢測BUN、Scr、24小時尿蛋白定量。流式細胞儀技術(shù)檢測外周血CD4+/CD8+、Th17細胞比例。HE染色和Masson染色行腎臟病理檢查。免疫組化法檢測腎組織中IL-17、Col-Ⅲ、NF-κBp65的表達情況。 結(jié)果：1.BUN、Scr比較：（1）治療前各組大鼠BUN之間比較，無顯著性差異（P0.05）。治療后，清腎顆粒組、百令膠囊組、模型組大鼠BUN均升高，與正常組及假手術(shù)組相比差異均有顯著性（P0.01）。假手術(shù)組大鼠BUN與正常組大鼠比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組和百令膠囊組大鼠BUN均較模型組低，差異有顯著性（P0.01）。清腎顆粒組大鼠BUN較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。（2）治療前各組大鼠Scr之間比較，無顯著性差異（P0.05）。治療后，清腎顆粒組、百令膠囊組、模型組大鼠Scr均升高，與正常組及假手術(shù)組相比差異均有顯著性（P0.01）。假手術(shù)組大鼠Scr與正常組大鼠比較，差異（P0.05）。清腎顆粒組和百令膠囊組大鼠Scr均較模型組低，差異有顯著性（P0.01）。清腎顆粒組大鼠Scr較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。2.24小時尿蛋白定量比較：百令膠囊組、模型組大鼠24小時尿蛋白定量均較正常組及假手術(shù)組高，差異有顯著性（P0.01）。清腎顆粒組大鼠24小時尿蛋白定量較正常組高，差異有顯著性（P0.01），清腎顆粒組大鼠24小時尿蛋白定量較假手術(shù)組高，差異有統(tǒng)計學意義（P0.05）。假手術(shù)組大鼠24小時尿蛋白定量與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組、百令膠囊組大鼠24小時尿蛋白定量均較模型組低，，差異有顯著性（P0.01）。清腎顆粒組大鼠24小時尿蛋白定量較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。3.外周血CD4+/CD8+比較：清腎顆粒組大鼠外周血CD4+/CD8+較正常組及假手術(shù)組高，差異有統(tǒng)計學意義（P0.05）。百令膠囊組、模型組大鼠外周血CD4+/CD8+均較正常組及假手術(shù)組高，差異有顯著性（P0.01）。假手術(shù)組大鼠外周血CD4+/CD8+與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組外周血CD4+/CD8+較模型組低，差異有顯著性（P0.01）。百令膠囊組外周血CD4+/CD8+較模型組低，差異有統(tǒng)計學意義（P0.05）。清腎顆粒組大鼠外周血CD4+/CD8+較百令膠囊組低，差異有顯著性（P0.01）。4.外周血Th17細胞比例比較：清腎顆粒組、百令膠囊組、模型組大鼠外周Th17細胞比例均較正常組及假手術(shù)組高，差異有顯著性（P0.01）。假手術(shù)組大鼠外周Th17細胞比例與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組大鼠外周Th17細胞比例較模型組低，差異有顯著性（P0.01）。百令膠囊組鼠外周Th17細胞比例較模型組低，差異有統(tǒng)計學意義（P0.05）。而清腎顆粒組大鼠外周Th17細胞比例較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。5.免疫組化IL-17染色比較：清腎顆粒組、百令膠囊組、模型組大鼠IL-17半定量評分均較正常組及假手術(shù)組高，差異有顯著性（P0.01）。假手術(shù)組大鼠IL-17半定量評分與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組、百令膠囊組大鼠IL-17半定量評分均較模型組低，差異有顯著性（P0.01）。而清腎顆粒組大鼠IL-17半定量評分較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。6.免疫組化NF-κBp65染色比較：清腎顆粒組、百令膠囊組、模型組大鼠NF-κBp65著色半定量評分均較正常組及假手術(shù)組高，差異有顯著性（P0.01）。假手術(shù)組大鼠NF-κBp65著色半定量評分與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組、百令膠囊組大鼠NF-κBp65著色半定量評分均較模型組低，差異有顯著性（P0.01）。而清腎顆粒組大鼠NF-κBp65著色半定量評分較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。7.免疫組化Col-Ⅲ染色比較：百令膠囊組、模型組大鼠Col-Ⅲ著色半定量評分均較正常組及假手術(shù)組高，差異有顯著性（P0.01），清腎顆粒組大鼠Col-Ⅲ著色半定量評分較正常組及假手術(shù)組高，差異有統(tǒng)計學意義（P0.05）。假手術(shù)組大鼠Col-Ⅲ著色半定量評分與正常組比較，差異無統(tǒng)計學意義（P0.05）。清腎顆粒組、百令膠囊組大鼠Col-Ⅲ著色半定量評分均較模型組低，差異有顯著性（P0.01）。而清腎顆粒組大鼠Col-Ⅲ著色半定量評分較百令膠囊組低，差異有統(tǒng)計學意義（P0.05）。 結(jié)論：1.免疫炎癥介導機制參與腎間質(zhì)纖維化的發(fā)生。2.清腎顆�？山档湍I纖維化大鼠血肌酐、尿素氮，減少24h尿蛋白定量。3.清腎顆�？蓽p輕腎纖維化大鼠腎臟病理損害，減輕炎癥反應(yīng)。4.清腎顆�？赏ㄟ^調(diào)節(jié)免疫功能紊亂，減少炎癥介質(zhì)IL-17的釋放，抑制NF-κB信號通路，減少NF-κBp65的釋放，下調(diào)腎纖維化大鼠腎間質(zhì)Col-Ⅲ的表達，減少腎間質(zhì)細胞外基質(zhì)（extracellular matrix,ECM）的沉積，從而起到拮抗腎臟纖維化（renal fibrosis,RF）的作用。
[Abstract]:Objective: To observe the changes of serum interleukin -17 (interleukin17, IL-17), nuclear factor kappa Bp65 (peripheral blood mononuclearcells, PBMC) in peripheral blood mononuclear cells (peripheral blood mononuclearcells, PBMC) and serum type III collagen (peripheral blood mononuclearcells, PBMC) in patients with chronic renal failure (chronic renal failure, CRF). The intervention of particles.
Methods: 68 cases of CRF damp heat syndrome were randomly divided into treatment group and 34 cases in control group, and 30 cases of health examination were selected as normal group. 7 cases were lost in the process (3 cases with Jinshui Po culling and 4 cases of capsule culling), 4 cases in the treatment group and 3 cases in the control group, 30 cases were completed and 30 cases in the treatment group were completed. There were 31 cases in the group. The treatment group was 18 men and 12 women, the average age (53.20 + 9.41) years, the control group of 19 men and 12 women, the average age (49.06 + 12.53) years old. The treatment group and the control group were given western medicine basic treatment and traditional Chinese medicine retention enema, the treatment group was added with the Qing Shen Granule, 3 times a day, 1 bags, course 8 weeks. Test the treatment group and the control group blood before and after treatment. The level of NF- kappa Bp65 in serum creatinine, Scr, IL-17, Col- III and PBMC was used to estimate the glomerular filtration rate (estimatedglomerular filtration rate, eGFR). The changes of TCM syndrome score in the two groups were observed before and after treatment.
Results: 1. comparison of the curative effect of clinical disease: the total effective efficiency of the treatment group was 86.67%, the control group was 58.06%, the two groups were statistically significant (P0.05).2. TCM syndrome curative effect comparison: the total effective rate of TCM syndrome in the treatment group was 86.67%, the control group was 45.16%, the two groups had significant difference (P0.01).3. TCM syndrome score Comparison: there was no significant difference between the TCM syndrome score of the treatment group and the control group (P0.05). The TCM syndrome score of the treatment group was significantly lower than that before the treatment (P0.01). The score of TCM syndrome was not statistically significant (P0.05) after the treatment in the control group, and the TCM syndrome score of the treatment group was significantly lower than that of the same group after treatment. Compared with the control group (P0.01).4.Scr and eGFR: (1) there was no significant difference in the level of Scr between the treatment group and the control group (P0.05). The Scr level in the treatment group was significantly lower than that before the treatment (P0.01). The level of Scr in the control group was not statistically significant (P0.05) after treatment (P0.05). The Scr level of the treatment group was lower than that of the same period after treatment. Group (P0.05). (2) there was no significant difference in the level of eGFR between the treatment group and the control group (P0.05). The level of eGFR in the treatment group was significantly higher than that before the treatment (P0.01). The difference of eGFR level in the control group was not statistically significant (P0.05) after treatment (P0.05). The level of eGFR in the treatment group was higher than that of the control group (P0.05).5. serum IL-17. Comparison: the level of serum IL-17 in the treatment group was higher than that of the normal group before treatment (P0.01). The serum IL-17 level in the control group was higher than that of the normal group before treatment (P0.01), but there was no significant difference between the treatment group and the control group before the treatment (P0.05). The serum IL-17 level in the treatment group was compared with that of the treatment group before treatment. The level of serum IL-17 in the control group was lower than that in the control group (P0.05). The difference was not statistically significant (P0.05). The level of serum IL-17 in the treatment group was lower than that in the control group (P0.05).6.PBMC NF- kappa Bp65: the NF- kappa Bp65 content in the treatment group was higher than that of the normal group before the treatment group, and the difference was significant (P0.01), and the control group before treatment. The content of NF- kappa Bp65 was higher than that of the normal group (P0.01), but there was no significant difference in the content of NF- kappa Bp65 in the treatment group and the control group before treatment (P0.05). The content of NF- kappa Bp65 in the treatment group was significantly lower than before the treatment (P0.01). There was no difference between the control group and the control group after the treatment. Statistical significance (P0.05), the content of NF- kappa Bp65 in the treatment group was significantly lower than that of the control group (P0.01).7. serum Col- III compared with the control group (P0.01).7. serum Col- III: the level of serum Col- III in the control group was higher than the normal group before treatment, and the difference was significant (P0.01). There was no statistical difference between the treatment group and the control group before the treatment of the serum Col- III level (P0.0). 5) the level of serum Col- III in the treatment group was significantly lower than that before treatment (P0.01). The level of serum Col- III in the control group was not statistically significant (P0.05) after treatment (P0.05). The level of serum Col- III in the treatment group was significantly lower than that in the control group (P0.01).
Conclusion: 1. Qing Shen kidney granules can improve the clinical symptoms of CRF damp heat syndrome, reduce Scr, increase the serum IL-17, NF- kappa Bp65 level of Col- III and PBMC in the patients with eGFR.2.CRF damp heat syndrome, and the.3. clear kidney granules can reduce the serum IL-17, Col- III and the level of nuclear kappa. After treatment, there was no abnormal change in safety index and no adverse drug reaction occurred during the treatment.
Objective: to detect the ratio of auxiliary T lymphocyte (CD4+T cell) to suppressive T lymphocyte (CD8+T cells) in peripheral blood of rats with unilateral ureteral obstruction (UUO) and the ratio of CD4+T cells to T lymphocyte (CD8+T cells) in the peripheral blood of renal interstitial fibrosis (RIF) rats. 17) the expression of nuclear factor kappa B-p65 (nuclear factors- kappa Bp65, NF- kappa Bp65), type III collagen (collagen type III, Col- III), to observe the disorder of immune function in UUO rats, the high release of inflammatory mediators and the intervention of clear kidney granules.
Methods: 60 healthy male SD rats were randomly divided into 5 groups according to the random area group method: the Qing Shen Granule group, the bailing capsule group, the model group, the sham operation group, the normal group, and 12 rats in each group. The blood urea nitrogen nitrogen (BUN) and the serum creatinine (serum creatinine, Scr) were detected before the experiment in each group. The difference was not statistically significant (P0.05). The results were not statistically significant (serum creatinine, Scr). The results were not statistically significant (P0.05). Group, bailing capsule group and model group were prepared with UUO method of renal interstitial fibrosis rat model. The three groups in the sham operation group separated the ureter but did not ligate the ureter before the sham operation group. The Qing Shen Granule group dissolved in the warm water of the 0.6g/100g in the warm water of 4ml, and the bailing capsule group dissolved in the warm water of 4ml in the warm water of 4ml, and the model group was treated with the bailing capsule group. The rats in the sham operation group and the normal group were intragastric with 4ml warm water. The rats in each group were intragastric every day for 3 weeks. The experimental rats were given the blood of the abdominal aorta and the left kidney after the end of the gavage of the stomach. The urine volume of the rats in each group was 24 hours before the operation. The biochemical method was used to detect the BUN, Scr, and 24 hour urine protein. The flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. The blood flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. The blood flow cytometry was used to detect CD4+/CD8+, Th17 cells of peripheral blood. Renal pathological examination was performed by proportional.HE staining and Masson staining. The expression of IL-17, Col- III and NF- kappa Bp65 in renal tissue were detected by immunohistochemistry.
Results: 1.BUN, Scr comparison: (1) there was no significant difference (P0.05) between the groups of rats before treatment (P0.05). After treatment, the BUN in the Qing Shen Granule group, bailing capsule group and the model group increased significantly (P0.01) compared with the normal group and the sham operation group (P0.01). There was no statistical difference between the rats of the artificial hand group and the normal group (P0.), the difference was not statistically significant (P0.). 05) the BUN in the Qing Shen Granule group and the bailing capsule group were lower than the model group, and the difference was significant (P0.01). The BUN of the Qing Shen Granule group was lower than the bailing capsule group, and the difference was statistically significant (P0.05). (2) there was no significant difference (P0.05) between the Scr in each group of rats before treatment (P0.05). After treatment, the Qing Shen Granule group, bailing capsule group, and the model group Scr were all Scr The difference was significant compared with the normal group and the sham operation group (P0.01). The Scr of the sham operation group was compared with the normal group (P0.05). The Scr of the Qing Shen Granule group and the bailing capsule group was lower than the model group (P0.01). The Scr of the Qing Shen granule group was lower than the bailing capsule group, and the difference was statistically significant (P0.05).2.24. The quantitative comparison of hourly urine protein: the 24 hour urine protein of the model group was higher than that of the normal group and the sham group (P0.01). The 24 hour urine protein of the rats in the Qing Shen Granule group was higher than the normal group (P0.01), and the urine protein of the rats in the group of Qing Shen Granule group was higher than that of the sham group, and the difference was higher than that of the sham operation group. There was statistical significance (P0.05). There was no significant difference between the 24 hours urine protein in the sham operation group and the normal group (P0.05). The urine protein in the Shishen group group and the bailing capsule group were lower than the model group (P0.01). The urine protein quantity of the rats in the Qing Shen Granule group was lower than that in the bailing group. The difference was that the urine protein was lower than that in the bailing group. Statistical significance (P0.05).3. peripheral blood CD4+/CD8+ comparison: the peripheral blood CD4+/CD8+ of the rats in the Qing Shen Granule group was higher than that of the normal group and the sham operation group, the difference was statistically significant (P0.05). The peripheral blood CD4+/CD8+ of the model group was higher than that of the normal group and the sham operation group, and the difference was significant (P0.01). The peripheral blood of the sham operation group was CD4+/CD8+. Compared with the normal group, the difference was not statistically significant (P0.05). The peripheral blood CD4+/CD8+ of the Qing Shen Granule group was lower than the model group (P0.01). The peripheral blood CD4+/CD8+ of the bailing capsule group was lower than the model group (P0.05). The peripheral blood CD4+/CD8+ of the Qing Shen Granule group was lower than that of the bailing capsule group, and the difference was significant (P0.01).4. outside the capsule group. The proportion of Th17 cells in peripheral blood was compared: the proportion of Th17 cells in the group of Qing Shen Granule group and bailing capsule group was higher than that of the normal group and the sham operation group, the difference was significant (P0.01). The proportion of peripheral Th17 cells in the sham operation group was not statistically significant compared with the normal group (P0.05). The proportion of the peripheral Th17 cells in the rats of the Qing Shen Granule group was more than that of the normal group (P0.05). The proportion of the model group was lower than that of the model group (P0.01). The percentage of Th17 cells in the capsule group was lower than that in the model group, and the difference was statistically significant (P0.05). The proportion of the peripheral Th17 cells in the rats of the Qing Shen Granule group was lower than that of the bailing capsule group. The difference was statistically significant (P0.05).5. immunohistochemical IL-17 staining comparison: the Qing Shen Granule group, the bailing capsule group, the model The semi quantitative score of IL-17 in the group of rats was higher than that in the normal group and the sham operation group (P0.01). There was no significant difference in the semi quantitative score of IL-17 in the sham operation group and the normal group (P0.05). The IL-17 semi quantitative score of the Qing Shen Granule group and the bailing capsule group was lower than that of the model group (P0.01). The semi quantitative score of IL-17 in rats was lower than that of Bailing capsule group, and the difference was statistically significant (P0.05).6. immunohistochemical NF- kappa Bp65 staining: the semi quantitative score of NF- kappa Bp65 coloring in the model group was higher than that of the normal group and the sham operation group, the difference was significant (P0.01). The coloring semi quantitative of NF- kappa Bp65 in the sham operation group was half quantitative. Compared with the normal group, the difference was not statistically significant (P0.05). The semi quantitative score of NF- kappa Bp65 coloring in the Qing Shen Granule group and the bailing capsule group was lower than the model group, and the difference was significant (P0.01). The NF- kappa Bp65 coloring semi quantitative score of the rats in the Qing Shen Granule group was lower than that in the bailing group, and the difference was statistically significant (P0.05).7. immunization Col- III Comparison: the Col- III coloring semi quantitative score of the model group was higher than that of the normal group and the sham group (P0.01). The Col- III coloring semi quantitative score of the rats in the Qing Shen Granule group was higher than that of the normal group and the sham operation group (P0.05). The semi quantitative score of Col- III coloring in the sham operation group was and was positive. The difference was not statistically significant (P0.05). The semi quantitative score of Col- III coloring in the group of Qing Shen Granule group and bailing capsule group was lower than that of the model group (P0.01), but the semi quantitative score of Col- III coloring in the rats of the Qing Shen Granule group was lower than that of the bailing group (P0.05).
Conclusion: 1. immuno inflammatory mediating mechanism participates in the pathogenesis of renal interstitial fibrosis,.2. clearing kidney granules can reduce the blood creatinine, urea nitrogen and the reduction of 24h urine protein quantitative.3. clearing kidney granules can reduce the renal pathological damage in renal fibrosis rats, and reduce the inflammatory response by regulating the immune dysfunction and reducing the inflammatory mediations. Release of mass IL-17, inhibition of NF- kappa B
【學位授予單位】：安徽中醫(yī)藥大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R692

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