發(fā)布時間：2018-04-24 12:29

  本文選題：晚期糖基化終產(chǎn)物 + 大鼠��； 參考：《西北農(nóng)林科技大學(xué)》2014年碩士論文

【摘要】：晚期糖基化終產(chǎn)物(Advanced Glycation End Products，AGEs)是食品加工和貯藏過程中由于發(fā)生美拉德反應(yīng)或脂肪氧化反應(yīng)而形成的產(chǎn)物。AGEs被認為是一類重要的尿毒癥毒素，是形成糖尿病并發(fā)癥的重要因素。目前AGEs對腎臟的生物學(xué)效應(yīng)研究，主要集中在人工合成AGEs對體外腎臟細胞或生物體的影響，膳食中AGEs對老鼠腎臟功能作用的研究較少。本試驗擬選用健康SD大鼠和糖尿病大鼠為動物模型，通過飼喂不同含量AGEs飼料，研究AGEs對腎臟功能的影響，分析AGEs在糖尿病和健康大鼠中各個器官中的分布，探究AGEs對大鼠血脂水平的影響，以更好的評估膳食AGEs的安全性。主要得出以下結(jié)論： (1)隨著時間的延長，AGEs能夠在健康大鼠血清與腎臟中累積，，且血清與腎臟中AGEs含量隨著AGEs攝入量的增加而升高。過多的AGEs攝入導(dǎo)致健康大鼠的腎臟功能受損，并導(dǎo)致腎臟氧化應(yīng)激水平升高。 (2)攝入的AGEs能夠在糖尿病大鼠血清和腎臟中累積，高糖引起的AGEs形成增多和腎功能降低引起的AGEs清除率降低是AGEs在糖尿病大鼠體內(nèi)累積的原因。攝入的AGEs使得糖尿病大鼠的氧化應(yīng)激水平增強。 (3)腎臟是膳食AGEs累積的重要器官。AGEs在健康大鼠和糖尿病大鼠各器官中含量由高到低為：腎＞肝＞心臟＞肺＞脾臟，隨著時間的延長，AGEs在各器官中的累積量增加，且隨攝入AGEs量的增加而升高。飼喂同一種飼料時，糖尿病大鼠中各器官的熒光性AGEs和CML均高于健康大鼠。 (4) AGEs能引起糖尿病大鼠和健康大鼠的血脂異常。隨著AGEs攝入量增加，血清中總膽固醇、甘油三酯及低密度脂蛋白的含量升高。糖尿病大鼠血清中總膽固醇、甘油三酯及低密度脂蛋白的含量均高于正常鼠，而高密度脂蛋白含量隨著AGEs攝入量的增加而降低。
[Abstract]:Advanced Glycation End products products (ages) is a product of Maillard reaction or fat oxidation during food processing and storage. Ages is considered to be an important uremic toxin and an important factor in diabetic complications. At present, the biological effects of AGEs on kidney are mainly focused on the effects of synthetic AGEs on renal cells or organisms in vitro, while the effects of AGEs on kidney function in mice are less studied in diet. In this experiment, healthy SD rats and diabetic rats were selected as animal models. The effects of AGEs on renal function were studied by feeding different contents of AGEs diet, and the distribution of AGEs in various organs of diabetic and healthy rats was analyzed. To explore the effect of AGEs on blood lipid level in rats to evaluate the safety of dietary AGEs. The main conclusions are as follows: 1) ages could accumulate in serum and kidney of healthy rats with time, and the content of AGEs in serum and kidney increased with the increase of AGEs intake. Excessive intake of AGEs resulted in impaired renal function and increased renal oxidative stress in healthy rats. (2) AGEs intake could accumulate in serum and kidney of diabetic rats. The increase of AGEs formation induced by high glucose and the decrease of AGEs clearance caused by decreased renal function were the reasons for the accumulation of AGEs in diabetic rats. Intake of AGEs increased oxidative stress levels in diabetic rats. 3) Kidney is an important organ of dietary AGEs accumulation. Ages content in the organs of healthy rats and diabetic rats is from high to low: kidney > liver > heart > lung > spleen. The accumulation of ages in each organ increases with time. And increased with the increase of AGEs intake. The fluorescence AGEs and CML of each organ of diabetic rats were higher than that of healthy rats. AGEs can cause hyperlipidemia in diabetic rats and healthy rats. As AGEs intake increased, serum total cholesterol, triglyceride and low density lipoprotein levels increased. The contents of total cholesterol, triglyceride and low density lipoprotein in serum of diabetic rats were higher than those of normal rats, while the content of high density lipoprotein decreased with the increase of AGEs intake.
【學(xué)位授予單位】：西北農(nóng)林科技大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：TS201.2;R692

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本文編號：1796594