本文選題：狼瘡性腎炎 + 調(diào)節(jié)性T淋巴細(xì)胞��； 參考：《福建中醫(yī)藥大學(xué)》2014年碩士論文

【摘要】：目的：通過檢測熱毒熾盛型和陰虛內(nèi)熱型狼瘡性腎炎患者外周血調(diào)節(jié)性T細(xì)胞、IL-10、IL-6、干擾素-α水平,探討微觀指標(biāo)與中醫(yī)證型及疾病活動性之間的關(guān)系,尋求狼瘡性腎炎中醫(yī)辨證的物質(zhì)基礎(chǔ),為探索中醫(yī)證型的本質(zhì)提供實驗證據(jù)。 方法：收集符合納入標(biāo)準(zhǔn)的熱毒熾盛型狼瘡性腎炎患者28例,陰虛內(nèi)熱型狼瘡性腎炎患者27例,健康對照組21例,應(yīng)用三聯(lián)熒光染色通過流式細(xì)胞儀分析外周血中Treg細(xì)胞水平,并用CBA流式蛋白分析系統(tǒng)檢測外周血中IL-10、IL-6、IFN-α水平。 結(jié)果： 1、熱毒熾盛組IL-10、IL-6水平明顯高于陰虛內(nèi)熱組(P0.01)；熱毒熾盛組Treg顯著低于陰虛內(nèi)熱組和正常組(P0.01),陰虛內(nèi)熱組和正常組比較Treg有顯著性差異(P0.05)；熱毒熾盛組IFN-α顯著高于陰虛內(nèi)熱組和正常組(P0.01),陰虛內(nèi)熱組和正常組比較IFN-α無顯著性差異(P0.05)。 2、狼瘡性腎炎患者外周血Treg與SLEDAI、IL-10、IL-6呈負(fù)相關(guān)(P0.05)。狼瘡性腎炎患者外周血IFN-α與SLEDAI、IL-10、IL-6呈正相關(guān)(P0.01)。Treg與IFN-α之間無相關(guān)性。 結(jié)論： 1、LN患者外周血Treg水平與正常組比較有顯著性差異,Treg減少可能是狼瘡性腎炎患者出現(xiàn)免疫功能異常的重要原因。 2、熱毒熾盛組LN患者Treg水平與陰虛內(nèi)熱組比較有顯著性差異,可作為區(qū)別兩證型的微觀指標(biāo)。 3、熱毒熾盛組LN患者IFN-α水平與陰虛內(nèi)熱組比較有顯著性差異,可作為區(qū)別兩證型的微觀指標(biāo)。 4、狼瘡性腎炎患者外周血Treg與SLEDAI、IL-10、IL-6呈負(fù)相關(guān),IFN-α與SLEDAI、 IL-10、IL-6呈正相關(guān)。因此IFN-α、IL-10、IL-6、Treg可作為狼瘡活動性的指標(biāo)。但I(xiàn)FN-α和Treg之間并沒有相關(guān)性。
[Abstract]:Objective: to investigate the relationship between microcosmic indexes and TCM syndromes and disease activity by detecting the levels of IL-10 / IL-6 and IFN- 偽 in peripheral blood regulatory T cells in patients with lupus nephritis with excessive heat toxin and internal heat of yin deficiency. To seek the material basis of TCM syndrome differentiation of lupus nephritis, to provide experimental evidence for exploring the essence of TCM syndrome type. Methods: Twenty-eight patients with heat toxic and incandescent lupus nephritis, 27 patients with heat type lupus nephritis due to yin deficiency and 21 healthy controls were collected. The levels of Treg cells in peripheral blood were analyzed by flow cytometry with triple fluorescent staining. The level of IL-6 and IFN-偽 in peripheral blood was detected by CBA flow protein analysis system. Results: 1. The level of IL-6 in heat toxic heat group was significantly higher than that in yin deficiency group (P 0.01), the Treg level in heat toxin heat group was significantly lower than that in yin deficiency group and normal group (P 0.01), there was significant difference in Treg between yin deficiency heat group and normal group (P 0.05), and the level of IFN- 偽 in heat toxin heat group was significantly higher than that in yin deficiency group. There was no significant difference in IFN- 偽 between the internal heat group and the normal group. 2. There was a negative correlation between Treg and IL-6 in patients with lupus nephritis. There was no positive correlation between IFN- 偽 and IL-6 in peripheral blood of patients with lupus nephritis. Conclusion: 1There was significant difference in the level of Treg in peripheral blood between LN patients and normal controls. The decrease of Treg may be an important reason for abnormal immune function in patients with lupus nephritis. 2. The level of Treg in LN group was significantly different from that in Yin deficiency group, and could be used as a microscopic index to distinguish the two syndromes. 3. The level of IFN- 偽 in LN group was significantly different from that in Yin-deficiency group, and could be used as a microscopic index to distinguish the two syndrome types. 4. There was a negative correlation between Treg and IL-6 in peripheral blood of patients with lupus nephritis and a positive correlation between IFN- 偽 and SLEDAI, IL-10- 6 in patients with lupus nephritis. Therefore, IFN- 偽 IL-10 and IL-6 Treg may be used as an indicator of lupus activity. But there is no correlation between IFN- 偽 and Treg.
【學(xué)位授予單位】：福建中醫(yī)藥大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2014
【分類號】：R593.242

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