本文選題：IgA腎病 + Klotho��； 參考：《延邊大學(xué)》2017年碩士論文

【摘要】：目的:研究Klotho蛋白在原發(fā)性IgA腎病(IgAN)患者血清及尿液中的表達(dá)水平及臨床意義。方法:選取2013年到2016年間24例在我院腎病學(xué)科住院,做腎穿刺檢查后診斷為原發(fā)性IgAN患者為研究對象,其中男15例,女9例,平均年齡49.13± 15.76歲。再選取我院10例健康體檢者作為對照組,其中男6例,女4例,平均年齡為44.80±13.61歲。對健康對照者和IgAN患者的血清、尿液分別采用酶聯(lián)免疫吸附試驗(ELISA)法檢測Klotho的表達(dá)水平。與此同時,收集此次研究對象的一般情況,包括收縮壓(SBP)、舒張壓(DBP)、身體質(zhì)量指數(shù)(BMI),再收集生化檢驗中的總膽固醇(CHO)、甘油三酯(TG)、低密度脂蛋白膽固醇(LDL-C)、高密度脂蛋白膽固醇(HDL-C)、尿素氮(BUN)、血肌酐(CREA)、血尿酸(UA)。本次數(shù)據(jù)采用SPSS23.0處理各數(shù)值的表示用均數(shù)±標(biāo)準(zhǔn)差,兩組間差異性的比較使用獨立樣本t檢驗,Pearson相關(guān)性分析Klotho蛋白與臨床數(shù)據(jù)間的相關(guān)性。結(jié)果:1.IgAN組中SBP、DBP、ALB、CHO、TG、LDI-C高于對照組,有顯著性差異(P0.01)。2.IgAN組血清和尿液Klotho 水平與健康對照組相比較降低,差異有統(tǒng)計學(xué)意義(P0.05)。3.IgAN 組患者血清 Klotho 水平與 DBP(r=0.471,P=0.020),CHO(1=0.483,P =0.017),LDL-C(r=0.526,?=0.008)呈負(fù)相關(guān)。結(jié)論:1.IgAN患者血清及尿液中Klotho 水平較健康對照組降低,提示Klotho的水平下降與IgAN的發(fā)展有關(guān)。Klotho水平降低可能通過激活RAS系統(tǒng)、引起炎癥反應(yīng)、損傷腎小球內(nèi)皮細(xì)胞、促進(jìn)腎臟纖維化等參與疾病的進(jìn)展。2.IgAN患者血清中Klotho水平與DBP、CHO、LDL-C呈負(fù)相關(guān),提示血清Klotho水平越高,高血壓和高血脂的程度越減輕,血壓和血脂可能在IgAN發(fā)展中起一定作用。其作用機(jī)制可能是Klotho通過直接分解脂肪細(xì)胞、減少脂質(zhì)沉積、減輕血管鈣化,還可直接激NO分泌、舒張血管,進(jìn)而減輕腎小球內(nèi)皮細(xì)胞損傷,延緩IgAN發(fā)展。
[Abstract]:Objective: to study the expression and clinical significance of Klotho protein in serum and urine of patients with primary IgA nephropathy.Methods: from 2013 to 2016, 24 patients (15 males and 9 females, mean age 49.13 鹵15.76 years) with primary IgAN diagnosed by renal puncture were selected.Ten healthy persons in our hospital were selected as the control group, including 6 males and 4 females, with an average age of 44.80 鹵13.61 years.The expression of Klotho in serum and urine of healthy controls and patients with IgAN were detected by Elisa.At the same time, collecting the general information of the subjects of this study,These include SBP, DBP, BMI, TGG, LDL-C0, HDL-CU, bun, creatinine creatinine creatinine (creatinine), serum uric acid (UAA).The difference between the two groups was compared using the independent sample t-test Pearson correlation analysis of the correlation between Klotho protein and clinical data.Results 1. The serum and urine Klotho levels in the IgAN group were significantly lower than those in the healthy control group. There was a negative correlation between the serum Klotho level and the serum Klotho level in the DBPr0.471P0.020 (P 0.017) LDL-C0. 5260. 008) group (P < 0. 017), and there was a negative correlation between the serum Klotho level and the serum Klotho level in the IgAN group (P = 0. 471, P = 0. 483P = 0. 017, P = 0. 483, P < 0. 05, P = 0. 008). The serum Klotho levels in the IgAN group were significantly lower than those in the healthy control group (P = 0. 05, P = 0. 05, P = 0. 05, P < 0. 05).Conclusion 1. The levels of Klotho in serum and urine of IgAN patients are lower than those of healthy controls, suggesting that the decrease of Klotho level is related to the development of IgAN. The decrease of Klotho level may cause inflammatory reaction and damage glomerular endothelial cells by activating RAS system.2. The level of serum Klotho in IgAN patients is negatively correlated with LDL-C, suggesting that the higher the level of serum Klotho, the lower the degree of hypertension and hyperlipidemia. Blood pressure and blood lipid may play a role in the development of IgAN.The mechanism may be that Klotho can directly decompose adipocytes, reduce lipid deposition, reduce vascular calcification, stimulate the secretion of no directly, relax blood vessels, and then alleviate the injury of glomerular endothelial cells and delay the development of IgAN.
【學(xué)位授予單位】：延邊大學(xué)
【學(xué)位級別】：碩士
【學(xué)位授予年份】：2017
【分類號】：R692.31

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本文編號：1750720