本文選題：絲裂霉素C　切入點：前列腺癌細(xì)胞　出處：《中國生物化學(xué)與分子生物學(xué)報》2017年02期

【摘要】：絲裂霉素C是一種廣譜抗腫瘤抗生素,對多種癌癥有抗癌作用,其作用原理可使細(xì)胞的DNA發(fā)生鏈間交聯(lián),引起DNA雙鏈斷裂,阻礙DNA的復(fù)制,從而抑制腫瘤細(xì)胞分裂。臨床上主要用于胃癌、腸癌、肝癌及胰腺癌等消化道癌方面的治療。本文研究絲裂霉素C對轉(zhuǎn)染人BLM解旋酶基因(shRNA載體)前后前列腺癌PC3細(xì)胞活性的影響。使用前期成功構(gòu)建的干擾載體轉(zhuǎn)染PC3細(xì)胞,在轉(zhuǎn)染48 h后加藥,通過熒光定量PCR、MTT法、Transwell小室實驗、細(xì)胞劃痕實驗、流式細(xì)胞術(shù),分別檢測加藥12、24、36 h BLM基因的表達(dá)量、PC3細(xì)胞增殖能力、侵襲能力、遷移能力及凋亡情況的變化。結(jié)果顯示,敲減BLM基因表達(dá)后的PC3細(xì)胞相對于正常PC3細(xì)胞其增殖能力、侵襲能力和遷移能力能顯著被絲裂霉素C抑制,且絲裂霉素C能顯著促進(jìn)其細(xì)胞的凋亡,說明BLM基因低表達(dá)的前列腺癌細(xì)胞對絲裂霉素C更敏感。研究結(jié)果為絲裂霉素C在前列腺癌的臨床治療上奠定了理論基礎(chǔ)。
[Abstract]:Mitomycin C is a broad-spectrum antitumor antibiotic, which has anticancer effect on many kinds of cancer. Its mechanism can cause interstrand crosslinking of DNA in cells, cause double strand break of DNA, block the replication of DNA, and thus inhibit the division of tumor cells.It is mainly used in the treatment of gastrointestinal cancer, such as stomach cancer, intestinal cancer, liver cancer and pancreatic cancer.The effect of mitomycin C on the activity of prostate cancer PC3 cells before and after transfection of human BLM helicase gene was studied.After 48 hours of transfection, PC3 cells were transfected with interference vector successfully constructed before transfection.The expression of BLM gene and the changes of proliferation, invasion, migration and apoptosis of PC3 cells were detected.The results showed that the ability of proliferation, invasion and migration of PC3 cells after knockout of BLM gene expression was significantly inhibited by mitomycin C, and mitomycin C could significantly promote the apoptosis of PC3 cells.It suggested that prostate cancer cells with low expression of BLM gene were more sensitive to mitomycin C.The results provide a theoretical basis for the clinical treatment of prostate cancer with mitomycin C.
【作者單位】： 貴州大學(xué)高原山地動物遺傳育種與繁殖省部共建教育部重點實驗室;貴州大學(xué)生命科學(xué)學(xué)院;貴州大學(xué)動物科學(xué)學(xué)院;
【基金】：國家自然科學(xué)基金(No.31361406)資助~~
【分類號】：R737.25

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