本文選題：番茄紅素　切入點(diǎn)：冷凍保存　出處：《吉林大學(xué)》2014年碩士論文

【摘要】：目的： 通過(guò)在精子冷凍保護(hù)液中添加不同濃度的番茄紅素，研究番茄紅素對(duì)精子冷凍損傷的保護(hù)作用及機(jī)制。 方法：選擇2013年9月至2014年3月來(lái)自吉林大學(xué)第一醫(yī)院吉林省人類精子庫(kù)捐精者的精液標(biāo)本，共計(jì)25份；采用計(jì)算機(jī)輔助精液常規(guī)參數(shù)分析（CASA）凍前和凍后精液標(biāo)本的精子前向運(yùn)動(dòng)力(a+b)、精子活動(dòng)率(a+b+c)；采用硫代巴比妥酸(TBA)比色法，測(cè)定凍后精子丙二醛（MDA）的含量，作為精子膜脂質(zhì)過(guò)氧化反應(yīng)程度的指標(biāo)；應(yīng)用原位末端轉(zhuǎn)移酶標(biāo)記技術(shù)（TUNEL）法通過(guò)激光共聚焦顯微鏡觀察精子DNA損傷情況，測(cè)定陽(yáng)性細(xì)胞比率作為反映凍后精子DNA完整性的指標(biāo)。利用JC-1標(biāo)記法通過(guò)激光共聚焦顯微鏡檢測(cè)線粒體膜電位水平，作為反映凍后精子線粒體損傷的指標(biāo)。 結(jié)果： 精液冷凍保存后各組的精子前向運(yùn)動(dòng)力和活力均比新鮮組精液的參數(shù)下降（P＜0.05)，，冷凍后添加不同濃度的番茄紅素其線粒體膜電位水平均明顯高于對(duì)照組（P＜0.05)。添加5mol/L番茄紅素組其TUNEL陽(yáng)性細(xì)胞比率顯著低于對(duì)照組（P＜0.05)。其余實(shí)驗(yàn)組的檢測(cè)指標(biāo)與對(duì)照組相比無(wú)明顯統(tǒng)計(jì)學(xué)意義（P0.05)。 結(jié)論：冷凍保存能夠使精子的移動(dòng)力、活力發(fā)生顯著下降，在精液中添加番茄紅素可以減少線粒體氧化損傷，精子冷凍保護(hù)液中添加適當(dāng)濃度的番茄紅素可以保護(hù)精子DNA的完整性。番茄紅素作為抗氧化劑，以適當(dāng)?shù)臐舛忍砑釉诰豪鋬霰Ｗo(hù)液中可以明顯改善精子質(zhì)量。
[Abstract]:Objective:. The protective effect and mechanism of lycopene on sperm cryopreservation injury were studied by adding lycopene with different concentrations. Methods: from September 2013 to March 2014, 25 semen samples were collected from donors from Jilin Province Human sperm Bank, first Hospital of Jilin University. The sperm motility and sperm motility were analyzed by computer aided semen routine parameters before and after freezing, and the content of malondialdehyde (MDAs) in frozen sperm was determined by thiobarbituric acid TBA colorimetry. The degree of lipid peroxidation of sperm membrane was determined by in situ terminal transferase labeling (Tunel) technique, and the DNA damage was observed by confocal laser microscopy. The ratio of positive cells was used as an index to reflect the integrity of DNA in frozen spermatozoa and the mitochondrial membrane potential level was detected by laser confocal microscope with JC-1 labeling method as an index to reflect the damage of sperm mitochondria after freezing. Results:. The sperm forward motility and motility of each group after cryopreservation were significantly lower than that of fresh semen (P < 0.05). The mitochondrial membrane potential level of lycopene added with different concentrations of lycopene after freezing was significantly higher than that of control group (P < 0.05). The percentage of TUNEL positive cells in lycopene group was significantly lower than that in control group (P < 0.05). Conclusion: cryopreservation can significantly decrease the motility and motility of spermatozoa, and the addition of lycopene in semen can reduce the oxidative damage of mitochondria. The integrity of sperm DNA could be protected by the addition of lycopene in the cryopreservation solution, and the quality of spermatozoa could be improved by adding lycopene as an antioxidant.
【學(xué)位授予單位】：吉林大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R698.2

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本文編號(hào)：1667568