本文選題：膀胱癌　切入點(diǎn)：基因多態(tài)性　出處：《南京醫(yī)科大學(xué)》2014年碩士論文

【摘要】：目的：研究NFKB1基因啟動(dòng)子-94ins/del ATTG與中國(guó)漢族人膀胱癌的相關(guān)性。 方法：采用Taqman-MGB方法檢測(cè)609例中國(guó)漢族人群膀胱癌患者與640例健康對(duì)照者NFKB1基因啟動(dòng)子-94ins/del ATTG基因型。運(yùn)用Logistic回歸模型評(píng)價(jià)單核苷酸多態(tài)性與膀胱癌危險(xiǎn)因素之間的相關(guān)性。采用實(shí)時(shí)定量聚合酶鏈反應(yīng)分析NFKB1mRNA的表達(dá)。 結(jié)果：攜帶del/del基因型的個(gè)體與攜帶ins/ins或ins/del基因型的個(gè)體相比,患膀胱癌的風(fēng)險(xiǎn)增加1.92倍(校正OR=1.92,95%CI=1.42-2.59).在分層分析中,攜帶del/del基因型的個(gè)體與攜帶ins/ins或ins/del基因型的個(gè)體相比患膀胱癌風(fēng)險(xiǎn)明顯增高的人群有：年齡超過(guò)65歲(OR=2.37,95%CI=1.52-3.70),男性(OR=1.97,95%CI=1.40-2.79),有腫瘤家族史(OR=3.59,95%CI=1.19-10.9),風(fēng)險(xiǎn)分別增加2.37倍、1.97倍、3.59倍。此外,攜帶del/del基因型的個(gè)體與攜帶ins/ins或ins/del基因型的個(gè)體相比,患非肌層浸潤(rùn)性膀胱癌(OR=2.07,95%CI=1.51-2.85)、膀胱癌I級(jí)(OR=2.40,95%CI=1.68-3.43)、單發(fā)膀胱癌(OR=2.04,95%CI=1.48-2.82)、較小膀胱腫瘤(OR=2.10,95%CI=1.51-2.92)的風(fēng)險(xiǎn)更高,風(fēng)險(xiǎn)分別增加2.07倍、2.40倍、2.04倍。NFKB1mRNA的表達(dá),在攜帶插入型純合子基因型的患者膀胱癌組織中比攜帶缺失型等位基因型患者膀胱癌組織中高。 結(jié)論：NFKB1基因啟動(dòng)子-94ins/del ATTG基因多態(tài)性可能是中國(guó)漢族人膀胱癌的致病因素。
[Abstract]:Aim: to study the association between NFKB1 gene promoter -94insdel ATTG and bladder cancer in Chinese Han nationality. Methods: the NFKB1 gene promoter -94ins-del ATTG genotype was detected by Taqman-MGB in 609 patients with bladder cancer in Chinese Han nationality and 640 healthy controls. Logistic regression model was used to evaluate the relationship between single nucleotide polymorphisms and risk factors of bladder cancer. Real-time quantitative polymerase chain reaction (PCR) was used to analyze the expression of NFKB1mRNA. Results: the risk of bladder cancer in individuals with del/del genotype was 1.92 times higher than that with ins/ins or ins/del genotype. The individuals with del/del genotype had significantly higher risk of bladder cancer compared with those with ins/ins or ins/del genotype: the risk of bladder cancer was 2.37%, 1.57% and 3.70%, 1.97% and 1.40-2.79%, respectively, and the risk of bladder cancer was 2.37 times 1.97 times 3.59 times higher than that of the individuals with ins/ins or ins/del genotype. In addition, the risk was 2.37 times 1.97 times and 3.59 times higher than that of the individuals with ins/ins or ins/del genotypes. In addition, the risk of bladder cancer was increased by 2.37 times or 3.59 times respectively, and the incidence of cancer was 1.595% 1.19-10.9% higher than that of the individuals with ins/ins or ins/del genotype. Compared with those with ins/ins or ins/del genotypes, individuals with del/del genotype had a higher risk of developing non-myometrial invasive bladder cancer, OR2.07-2.85, OR2.4095CI1.68-3.43C, single bladder cancer 2.0495CI1.48-2.82, and small bladder cancer 2.1095CIT 1.51-2.92. The risk was 2.07 times 2.40 times 2.04 times higher than that of 2.1095 CI 1.51-2.92, respectively, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/del genotype or ins/ins genotype, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, and the risk was 2.07 times 2.40 times 2.04 times higher than that of the individuals with ins/ins or ins/del genotypes, respectively. More bladder cancer tissues were found in patients with insertion homozygous genotypes than those with deletion alleles. Conclusion the gene polymorphisms of -94 ins / del ATTG gene of the 10% NFKB1 gene may be the pathogenetic factor of bladder cancer in Chinese Han people.
【學(xué)位授予單位】：南京醫(yī)科大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類(lèi)號(hào)】：R737.14

【參考文獻(xiàn)】

相關(guān)期刊論文 前1條

1 ;MTHFR C677T polymorphisms are associated with aberrant methylation of the IGF-2 gene in transitional cell carcinoma of the bladder[J];Journal of Biomedical Research;2012年02期

，

本文編號(hào)：1664098