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  本文選題：巨噬細胞　切入點：腎結石晶體　出處：《廣西醫(yī)科大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的:研究體外細胞培養(yǎng)體系中人巨噬細胞U937與腎結石晶體的相互作用，觀察細胞與晶體反應的形態(tài)學改變，并探討巨噬細胞在腎結石形成中的可能作用。 方法:體外利用PMA（Phorbol-12-myristate-13-acetate，，PMA)對人單核細胞株U937進行誘導，激活分化為巨噬細胞后，并使用不同種類的腎結石晶體與之進行共培養(yǎng)。分別運用倒置相差顯微鏡、掃描電子顯微鏡及活細胞成像技術—顯微延時攝影靜態(tài)及動態(tài)地觀察對細胞與晶體的相互作用。根據細胞與不同種類晶體共培養(yǎng)的時段不同分組，采用茜素紅S—氯化十六烷基吡啶鈣鹽半定量測定法測定晶體-細胞反應后細胞基質的鈣鹽沉積程度。 結果:光鏡、掃描電鏡下可見巨噬細胞吸附、吞噬晶體�；罴毎上窦夹g—顯微延時攝影捕捉到細胞吸附、吞噬、溶解及轉運晶體的動態(tài)過程。茜素紅S—氯化十六烷基吡啶鈣鹽半定量測定法：羥基磷灰石（hydroxyapatite，HAP）與一水草酸鈣（calcium oxalate monohydrate，COM）混合實驗組與其它組在相同時段細胞基質的鈣鹽沉積程度差別有統(tǒng)計學意義（P＜0.05），24小時組的程度最高；HAP組與空白對照組在相同時段差別有統(tǒng)計學意義（P＜0.05），與COM組差別無統(tǒng)計學意義（P＞0.05）�？瞻讓φ战M與COM組在相同時段差別無統(tǒng)計學意義（P＞0.05）；各晶體實驗組12小時組與24小時組后差別有統(tǒng)計學意義（P＜0.05）,6小時組分別與另外兩個時段組差別無統(tǒng)計學意義（P＞0.05）；不同實驗組不同時段不存在交互作用（P＞0.05）。結論：巨噬細胞具有粘附、吞噬及轉運腎結石晶體的能力。HAP可能存在介導巨噬細胞增強吞噬HAP和COM的作用；巨噬細胞可能通過吞噬HAP后啟動吞噬COM。
[Abstract]:Aim: to study the interaction between human macrophage U937 and renal stone crystals in vitro, observe the morphological changes of cell and crystal reaction, and explore the possible role of macrophages in the formation of renal calculi. Methods: human mononuclear cell line U937 was induced by PMA-Phorbol-12-Eritrestate-13-acetatePMA in vitro, then activated and differentiated into macrophages and co-cultured with different kinds of kidney stone crystals. Scanning electron microscopy and living cell imaging techniques-static and dynamic observation of the interaction between cells and crystals by time-delay photography. The calcium deposition degree of cell matrix after crystal-cell reaction was determined by alizarin red S-cetylpyridine calcium salt semi-quantitative method. Results: under light microscope, macrophage adsorption and phagocytosis were observed under scanning electron microscope. Dynamic process of dissolution and transport of crystals. Semi-quantitative determination of alizarin red S- cetylpyridine chloride calcium salt: hydroxyapatite HAP) and calcium oxalate monohydrate monohydrate monohydrate. There was significant difference in sedimentation degree between HAP group and blank control group at the same time period (P < 0.05), but there was no significant difference between HAP group and COM group (P > 0.05). The difference between blank control group and COM group was at the same time. There was no statistical significance (P > 0.05), there was no significant difference between the experimental group and the other two groups after 12-hour group and 24-hour group (P < 0.05), there was no significant difference between the other two groups (P > 0.05), and there was no interaction between the experimental group and the other two groups (P > 0.05). Conclusion: macrophages have adhesion. The ability of phagocytosis and transport of renal stone crystals. HAP may mediate macrophages to enhance phagocytosis of HAP and COM, and macrophages may initiate phagocytosis of HAP and COM by phagocytosis of HAP.
【學位授予單位】：廣西醫(yī)科大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R692.4

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相關期刊論文 前1條

1 李宇琳;周恒;劉廣鵬;崔磊;;茜素紅半定量測定細胞基質鈣含量的實驗研究[J];組織工程與重建外科雜志;2008年02期

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