發(fā)布時間：2018-02-15 12:49

  本文關鍵詞： 動脈粥樣硬化 腎病 超敏C反應蛋白 大鼠　出處：《青海大學》2014年碩士論文　論文類型：學位論文

【摘要】：目的研究主要通過構(gòu)建動脈粥樣硬化性腎病模型，分別檢測兩組大鼠hs-CRP及腎功能（BUN、CREA）水平，了解大鼠hs-CRP水平與動脈粥樣硬化性腎病病程的關系。 方法選取40只健康成年雄性Wistar大鼠，將其隨機分為2組：對照組和模型組（動脈粥樣硬化性腎病組，AS組），對照組20只，模型組20只，造模組采用高脂飼料喂養(yǎng)+維生素D3腹腔注射方法構(gòu)建動脈粥樣硬化性腎病大鼠模型，造模周期為12周。各組分別于造模0周、造模6周、造模12周采集血標本測定血脂四項、腎功能（肌酐、尿素氮）及hs-CRP水平；造模結(jié)束后處死大鼠，制作主動脈、腎動脈及腎組織切片觀察病理變化。 結(jié)果造模6周及12周模型組血清hs-CRP水平分別為0.015±0.002、0.032±0.006，均高于對照組（P0.05）；6周模型組尿素氮及肌酐分別為10.33±0.81、46.8±1.3,12周模型組尿素氮及肌酐分別為12.58±0.48、72.4±2.2，均明顯高于對照組（P0.05）；且6周和12周hs-CRP與BUN、CREA均呈顯著正相關，6周r分別為0.606、0.947（P0.01），12周r分別為0.911、0.945（P0.01）。 結(jié)論同一時期中，模型組較對照組血清hs-CRP含量增高，hs-CRP水平與腎功能指標呈顯著正相關，隨著hs-CRP的升高，血清尿素氮、肌酐含量隨之升高，hs-CRP水平與動脈粥樣硬化性腎病病程相關，hs-CRP值越高病情越重。
[Abstract]:Objective to study the relationship between hs-CRP level and the course of atherosclerotic nephropathy (Atherosclerosis nephropathy) in two groups by measuring the levels of hs-CRP and renal function BUNCREAA. Methods Forty healthy adult male Wistar rats were randomly divided into two groups: control group and model group (atherosclerotic nephropathy group, control group, control group, model group, n = 20). The rat model of atherosclerotic nephropathy was established by intraperitoneal injection of vitamin D _ 3 with high fat diet. The model period was 12 weeks. The blood samples were collected at 0 weeks, 6 weeks and 12 weeks to determine the blood lipid in each group. Renal function (creatinine, urea nitrogen) and hs-CRP level, the rats were killed after model making, and the pathological changes of aorta, renal artery and renal tissue were observed. Results the serum hs-CRP levels in the model group were 0.015 鹵0.002 鹵0.032 鹵0.006, respectively, which were higher than those in the control group at 6 weeks (P 0.05) and the levels of urea nitrogen and creatinine in the model group were 10.33 鹵0.81C, 46.8 鹵1.3N and 12.58 鹵0.4872.4 鹵2.2respectively, which were significantly higher than those in the control group at 6 weeks and 6 weeks, respectively, and the levels of creatinine in the model group were significantly higher than those in the control group at the 6th week and the 6th week, and the creatinine levels in the model group were 12.58 鹵0.4872.4 鹵2.2, respectively. At 12 weeks, there was a significant positive correlation between hs-CRP and BUNA at 6 weeks (r = 0. 606, P 0. 047, P 0. 01, P 0. 01, r = 0. 911, P 0. 045, P 0. 01, r = 0. 911, P 0. 01, P 0. 01, respectively). Conclusion in the same period, the level of serum hs-CRP in the model group was significantly higher than that in the control group, and the level of hs-CRP was positively correlated with the renal function index. With the increase of hs-CRP, the serum urea nitrogen level was significantly increased. Creatinine content increased and the level of hs-CRP was associated with the course of atherosclerotic nephropathy. The higher the level of hs-CRP was, the more serious the disease was.
【學位授予單位】：青海大學
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：R543.5;R692

【參考文獻】

相關期刊論文 前10條

1 楊鵬遠,芮耀誠,焦亞斌;動脈粥樣硬化大鼠實驗模型的建立[J];第二軍醫(yī)大學學報;2003年07期

2 歐琴;;C反應蛋白檢測在評估兒童原發(fā)性腎病綜合征病情及預后中的價值[J];當代醫(yī)學;2013年35期

3 邱清芳,丁兆明,王波濤;冠心病患者C-反應蛋白檢測的意義[J];河北醫(yī)藥;2003年03期

4 李鳳蓮;杜麗新;李書清;劉蘭鳳;;急性腦梗死患者血清超敏C反應蛋白和白細胞介素10水平變化及臨床意義[J];檢驗醫(yī)學與臨床;2011年08期

5 郭延松,楊軍珂;鼠動脈粥樣硬化模型的建立[J];中國動脈硬化雜志;2003年01期

6 劉煥皓;;C反應蛋白在腎臟疾病中的臨床應用[J];內(nèi)科;2007年04期

7 張曉慧;李光韜;張卓莉;;C反應蛋白與超敏C反應蛋白的檢測及其臨床意義[J];中華臨床免疫和變態(tài)反應雜志;2011年01期

8 劉曉燕;鐘一紅;劉紅;袁敏;章曉燕;傅辰生;陳利民;滕杰;吉俊;鄒建州;丁小強;;慢性腎臟病患者氧化應激狀態(tài)及其相關影響因素[J];上海醫(yī)學;2009年09期

9 胡暉;;C-反應蛋白的臨床應用價值[J];中國實用內(nèi)科雜志;2007年S1期

10 劉之蕙;孟群;沈穎;;超敏C-反應蛋白在兒童慢性腎臟病中的應用價值[J];實用兒科臨床雜志;2009年17期

，

本文編號：1513302