本文關(guān)鍵詞： 膀胱腫瘤 基因治療 細(xì)胞毒藥物　出處：《中國老年學(xué)雜志》2017年15期 　論文類型：期刊論文

【摘要】：目的探討血管內(nèi)皮生長因子小干擾RNA(si VEGF)增敏吉西他濱(GEM)對人膀胱癌T24細(xì)胞株在小鼠膀胱成瘤情況的影響及其對瘤細(xì)胞生長的治療作用。方法采用細(xì)胞移植法分別把經(jīng)過處理(治療組)和未經(jīng)過處理(對照組)的T24細(xì)胞接種到小鼠膀胱內(nèi),然后用256排極速CT定期檢測,并在28 d時(shí)處死動(dòng)物稱瘤重量,測定瘤體積,進(jìn)行組織病理學(xué)和細(xì)胞學(xué)檢查。結(jié)果治療組和對照組的成瘤率分別為40%(6/15)和93%(14/15),治療組瘤細(xì)胞生長速度明顯低于對照組(P0.05)。兩組腫瘤重量和體積比較差異有統(tǒng)計(jì)學(xué)意義(P0.05)。組織病理學(xué)和細(xì)胞學(xué)檢查結(jié)果顯示,治療組移植瘤細(xì)胞增殖能力減弱,惡性度下降,瘤細(xì)胞生長轉(zhuǎn)移風(fēng)險(xiǎn)降低,而對照組則未出現(xiàn)相應(yīng)的變化。結(jié)論模擬人的小鼠膀胱癌原位模型更接近人體膀胱腫瘤的微環(huán)境和向膀胱腔內(nèi)生長的特征,故更適合臨床膀胱癌的治療研究。推測靶向基因治療聯(lián)合細(xì)胞毒藥物的化療策略極有可能為提升目前臨床膀胱癌的治療水平和提高膀胱癌病人的生存率帶來幫助。
[Abstract]:Objective to investigate the sensitizing effect of vascular endothelial growth factor (VEGF) small interfering RNA(si (VEGF) on gemcitabine (GEM). Effect of human bladder cancer cell line T24 on bladder tumorigenesis in mice and its therapeutic effect on tumor cell growth. Methods treated (treatment group) and untreated (control group) were treated by cell transplantation. T24 cells were inoculated into the bladder of mice. The animals were killed at 28 days to weigh the tumor and measure the tumor volume. Histopathological and cytological examinations were performed. Results the tumorigenesis rates in the treatment group and the control group were 40 / 15 and 93 / 14 / 15, respectively. The growth rate of tumor cells in the treatment group was significantly lower than that in the control group (P 0.05). There was a significant difference in tumor weight and volume between the two groups. The histopathological and cytological results showed that the tumor growth rate in the treatment group was significantly lower than that in the control group. In the treatment group, the proliferative ability of transplanted tumor cells decreased, the degree of malignancy decreased, and the risk of tumor cell growth and metastasis decreased. Conclusion the in-situ model of human bladder cancer in mice is closer to the microenvironment of human bladder tumor and the characteristics of bladder cavity growth. Therefore, this study is more suitable for the treatment of bladder cancer. It is speculated that the chemotherapeutic strategy of targeted gene therapy combined with cytotoxic drugs may help to improve the current treatment level of bladder cancer and improve the survival rate of bladder cancer patients. .
【作者單位】： 吉林大學(xué)第二醫(yī)院泌尿外科;
【基金】：吉林省自然科學(xué)基金項(xiàng)目(No.20150101195JC)
【分類號(hào)】：R737.14
【正文快照】： 腫瘤微環(huán)境對腫瘤的發(fā)生發(fā)展有著至關(guān)重要的影響。研究膀胱癌的理想動(dòng)物模型除應(yīng)與人體膀胱癌在組織結(jié)構(gòu)和生物學(xué)行為上相似外,腫瘤還應(yīng)生長在膀胱腔內(nèi)(原位生長)[1,2]。本實(shí)驗(yàn)選用小鼠,把經(jīng)過血管內(nèi)皮生長因子小干擾RNA(si VEGF)表達(dá)質(zhì)粒轉(zhuǎn)染并在其內(nèi)加進(jìn)細(xì)胞毒藥物吉西他濱(，

本文編號(hào)：1463150