【摘要】：1.目的 研究蒺藜提取物對大強度耐力運動大鼠運動能力的影響及其機制。 2.方法 32只SD大鼠隨機分為4組：安靜對照組（C，n=8）、蒺藜組（TT，n=8）、運動組（E，n=8）和蒺藜+運動組（TT+E，n=8）。安靜對照組和運動組大鼠自由飲用純水，而蒺藜組和蒺藜+運動組大鼠自由飲用蒺藜提取物配成的蒺藜飲料，記錄每日大鼠的飲用量（大鼠攝取的蒺藜提取物在120mg/kg體重左右）。運動大鼠進行大強度的五周遞增負荷運動，每周運動6天。5周耐力運動的倒數(shù)第二天進行30min的強度為30m/min的跑臺運動，斷尾取血，檢測運動前和運動后30min、2h的血糖水平。5周耐力運動的最后一天，各組大鼠進行一次力竭運動。力竭運動結(jié)束后36h，大鼠麻醉后下腔靜脈取血，檢測血漿睪酮(Testosterone，T)水平、胰島素樣生長因子1（Insulin-like growth factor1，IGF-1）水平、血紅細胞數(shù)（RBC）、血紅蛋白含量（Hb）。大鼠處死后取腓腸肌、比目魚肌，秤重，用BCA試劑盒檢測總蛋白含量；用western blot方法檢測雄激素受體（Androgenreceptor，AR）、胰島素樣生長因子1受體（Insulin-like growth factor receptor，IGF-1R）和肌球蛋白重鏈（Mysion heavy chain，MHC）的蛋白水平。 3.結(jié)果 1）蒺藜提取物提高大強度耐力運動大鼠的運動能力 （1）蒺藜提取物減輕大強度耐力運動大鼠體重降低的程度 實驗前各組大鼠體重無顯著性差異。從第三周開始，運動組、蒺藜+運動組大鼠的體重顯著低于安靜對照組（p0.05），該作用一直持續(xù)至實驗結(jié)束（p0.01）。與運動組比較，蒺藜+運動組大鼠的體重在第五周時顯著增加（p0.05）。此外，蒺藜組大鼠的體重與安靜對照組相比，在各周都無顯著性差異。 （2）蒺藜提取物提高大強度耐力運動大鼠的一次力竭運動時間 與對照組相比，蒺藜組大鼠的一次力竭運動時間無顯著差異。五周耐力運動后，蒺藜+運動組大鼠的一次力竭運動時間比運動組大鼠顯著延長（p0.01），約延長了63.2%。此外，運動訓練大鼠的一次力竭運動時間比對照組顯著延長（p0.05）。 2）蒺藜提取物提高大強度耐力運動大鼠運動能力的機制 （1）蒺藜提取物提高大強度耐力運動大鼠的血漿睪酮水平和腓腸肌AR的蛋白水平 與安靜對照組相比，運動組大鼠的血漿睪酮水平顯著降低（p0.01）；蒺藜+運動組大鼠的睪酮水平與運動組比較顯著增加（p0.01），與對照組相比無顯著差異，表明蒺藜提取物能完全逆轉(zhuǎn)大強度運動所造成的血睪酮水平降低。此外，與安靜組相比較，運動組大鼠腓腸肌的AR蛋白含量無顯著性差異。蒺藜+運動組大鼠腓腸肌的AR蛋白表達水平比運動組的顯著升高（p0.05）。 （2）蒺藜提取物提高大強度耐力運動大鼠的血漿IGF-1水平和腓腸肌IGF-1R的蛋白水平 與安靜對照組相比，運動組大鼠血漿的IGF-1的水平?jīng)]有顯著性差異。蒺藜+運動組大鼠的IGF-1水平與運動組相比較顯著增加（p0.05），表明補充蒺藜提取物能提高大強度運動大鼠血漿的IGF-1水平。此外，運動組與安靜組相比較，腓腸肌IGF-1R蛋白含量沒有顯著性差異，蒺藜+運動組與運動組相比較，腓腸肌IGF-1R蛋白表達水平顯著升高（p0.05）。 （3）蒺藜提取物增加大強度耐力運動大鼠腓腸肌的總蛋白濃度和MHC的蛋白水平 與對照組相比，運動組大鼠比目魚肌和腓腸肌的相對重量均顯著增加、且腓腸肌的蛋白濃度也顯著增加；而蒺藜組大鼠的比目魚肌和腓腸肌的相對重量、蛋白濃度均無顯著差異。與運動組比較，蒺藜+運動組大鼠腓腸肌的蛋白濃度顯著增加、但腓腸肌的相對重量以及比目魚肌的蛋白濃度都沒有顯著性差異，而比目魚肌相對重量卻比較意外地下降（p0.05）。此外，與安靜組相比，運動組的MHC蛋白水平?jīng)]有顯著性差異。與運動組相比，蒺藜+運動組的MHC蛋白水平顯著增高（p0.05）。 （4）藜提取物增加大強度耐力運動和不運動大鼠的一次力竭運動后30min的血糖水平 蒺藜提取物能降低運動和不運動大鼠運動前的血糖水平。蒺藜組與安靜組比較、蒺藜+運動組與運動組比較，血糖水平在一次力竭運動后的30min顯著增加（分別是p0.05，p0.01），但在運動后2h，各組血糖水平無顯著差異。 （5）蒺藜提取物對大強度耐力運動和不運動大鼠的Hb、紅細胞數(shù)無影響 4.結(jié)論： 1）蒺藜提取物延長大強度耐力運動大鼠的一次力竭運動時間，提高其運動能力。 2）蒺藜提取物增強大強度耐力運動大鼠運動能力的機制可能與其增加睪酮/AR水平和IGF-1/IGF-1R水平，，從而使腓腸肌的蛋白濃度、MHC蛋白水平增加，以及增加一次力竭運動后30min的血糖水平有關(guān)。 3）蒺藜提取物增強大強度耐力運動大鼠運動能力的機制與Hb和RBC數(shù)無關(guān)。
[Abstract]:1. Purpose The effect of the extract of the extract on the exercise ability of the large-strength endurance exercise rats and its machine System. 2. Methods 32 SD rats were randomly divided into 4 groups: the quiet control group (C, n = 8), the control group (TT, n = 8), the exercise group (E, n = 8), and the Astragalus + exercise group (TT + E). , n = 8). The quiet control group and the exercise group rats were free to drink pure water, while the rats in the control group and the exercise group were free to drink pure water, and the drinking quantity of the daily rats (the rat's uptake of the ginkgo leaf extract at 120 mg/ kg) was recorded. body weight). The exercise rats were subjected to five-week incremental load exercise with a large intensity, and the exercise was carried out for 6 days a week. The last day of the 5-week endurance exercise was performed with a treadmill exercise of 30 m/ min for 30 minutes, the blood glucose level of 2 hours was measured at 30 min and 2 hours before and after the exercise, and the 5-week endurance exercise One day of the last day, each group of rats The level of serum testosterone (T), insulin-like growth factor 1 (IGF-1), blood red blood cell count (RBC) and hemoglobin were measured at 36 h after the end of exhaustive exercise. (Hb). After the rats were sacrificed, the gastrocnemius muscle, the flounder muscle and the weight were weighed, and the total protein content was detected by the BCA kit; the androgen receptor (AR), the insulin-like growth factor receptor (IGF-1R) and the myosin heavy chain (MHC) were detected by western blot. protein water Ping.3. Results 1) The extract of the extract of the extract of the invention has the advantages of high strength, The exercise ability (1) of the force-moving rats to reduce the high-strength resistance to the extract Experimental study on the degree of weight loss in rats with force exercise There was no significant difference in the weight of the rats in the first group. From the third week, the weight of the rats in the exercise group and the group of the rats of the positive group was significantly lower than that of the quiet control group (p0.05), and the weight of the rats was always the same as that of the control group (p0.05). continued until the end of the experiment (p0.01). In comparison with the exercise group, the body weight of the baboon + exercise group was at the fifth Significant increases at week (p0.05). In addition, the body weight and the quiet control group in the treatment group There was no significant difference in each week. The time of exhaustive exercise of endurance sports rats was compared with that of the control group. There was no significant difference in the time of exhaustive exercise of the rats in the group. After the five-week endurance exercise, the time of exhaustive exercise of the rats in the group of exercise group and exercise group was significantly longer than that of the rats in the exercise group. (p0.01), about 63.2%. In addition, the exercise training rats' exhaustive exercise The time was significantly longer than that in the control group (p0.05). A mechanism (1) to improve the exercise capacity of a large-strength endurance exercise rat by taking the object (1) to improve the strength and endurance of the extract Plasma Testosterone in Exercise Rats Compared with the control group, the level of serum testosterone and the level of serum testosterone in the control group were significantly lower than that of the control group (p0.01), and the level of testosterone in the rats of the group of the rats of the group of the group of the rats of the group of the group of the group of the group of the group of the group of the group of the control group was significantly higher than that of the control group (p0.01), and there was no significant difference in the level of testosterone and the control group, indicating that the level of the serum testosterone of the rats in the exercise group The product can completely reverse the level of blood testosterone caused by the large-intensity exercise. In addition, compared with the quiet group, There was no significant difference in the AR protein content of the gastrocnemius in the exercise group. The expression of R-protein was significantly higher than that of the exercise group (p0.05). (2) The extract of Astragalus extract increased the exercise of large-intensity endurance. The level of IGF-1 and the level of IGF-1R in the gastrocnemius of the rat and the quiet control There was no significant difference in the level of IGF-1 in the rats in the exercise group compared with the group. In addition, the content of IGF-1R in the gastrocnemius was not significantly different from that of the quiet group, and the level of IGF-1R in the control group was compared with that of the exercise group. The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnemius was significantly higher (p0.05). (3) The expression of IGF-1R in gastrocnem@@ Compared with the control group, the total protein concentration of the gastrocnemius muscle and the protein level of MHC in the rats with high-intensity endurance exercise were higher than that of the control group. The relative weight of the fish muscle and the gastrocnemius was significantly increased, and the protein concentration of the gastrocnemius was also increased. The relative weight and protein concentration of the soleus muscle and the gastrocnemius muscle in the rats were not significantly different from those in the exercise group, but the relative weight of the gastrocnemius and the protein concentration of the soleus muscle were not increased. There was a significant difference, but the relative weight of the soleus was reduced unexpectedly (p0.05). 5). In addition, there was no significant difference in the level of MHC protein in the exercise group compared to the quiet group. Compared with the exercise group, the level of MHC protein was significantly higher in the + exercise group (p0.05). (4) The level of MHC protein in the exercise group was significantly higher than that of the exercise group (p0.05). After one-time exhaustive exercise of the high-intensity endurance exercise and the non-moving rats The blood glucose level of 0 min can decrease the blood glucose level before the exercise and the non-moving rats. The blood glucose level is compared with the quiet group, and the blood sugar level is significantly increased at 30 min after the exhaustive exercise. The blood glucose levels in each group were not significant after 2 h after exercise, respectively (p0.05, p0.01). The difference. (5) The amount of Hb and red blood cells in the high-strength endurance exercise and the non-moving rats were not affected by the extract of the extract. Conclusion:1) The mechanism of enhancing the exercise ability of the rats with high-intensity endurance exercise may increase the level of testosterone/ AR and the level of IGF-1/ IGF-1R. the protein concentration of the gastrocnemius muscle, the level of the MHC protein,
【學位授予單位】：上海體育學院
【學位級別】：碩士
【學位授予年份】：2014
【分類號】：G804.2

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