【摘要】：目的:通過(guò)研究不同高度絲素蛋白支架體內(nèi)原位修復(fù)兔膝關(guān)節(jié)骨軟骨缺損的修復(fù)機(jī)制,為臨床應(yīng)用提供研究基礎(chǔ)。方法:將11只新西蘭大白兔隨機(jī)分為2組,包括高支架組(LONG,材料平關(guān)節(jié)面)、低支架組(SHORT,材料低于軟骨下骨板)。在雙側(cè)膝關(guān)節(jié)股骨滑車處制造骨軟骨缺損(深度3.5~4.0 mm,直徑4.0 mm),將2種支架分別植入雙側(cè)膝骨軟骨缺損處。術(shù)后6周和12周時(shí)麻醉并處死動(dòng)物,行大體形態(tài)觀察、組織學(xué)觀察及力學(xué)測(cè)試。結(jié)果:經(jīng)過(guò)結(jié)構(gòu)和功能的綜合觀察評(píng)估,低支架組中骨軟骨缺損的修復(fù)效果優(yōu)于高支架組。低支架所修復(fù)的關(guān)節(jié)面更平整,軟骨下骨板生長(zhǎng)更厚實(shí)且力學(xué)性能更優(yōu)越。結(jié)論:低于軟骨下骨板的絲素蛋白支架能較好地促進(jìn)關(guān)節(jié)骨軟骨缺損的修復(fù),一定程度上提示著骨軟骨支架新的應(yīng)用方式。
[Abstract]:Aim: to study the mechanism of in situ repair of rabbit knee osteochondral defects with silk fibroin scaffolds in order to provide a basis for clinical application. Methods: eleven New Zealand white rabbits were randomly divided into two groups, including elevated stent group (LONG, material flat articular surface) and low stent group (SHORT, material lower than subchondral bone plate). Osteochondral defects were made at the femoral trochlear of both knee joints (depth 3.5 脳 4.0 mm, diameter 4.0 mm),). Two kinds of scaffolds were implanted into bilateral knee osteochondral defects respectively. The animals were anesthetized and killed at 6 and 12 weeks after operation. Gross morphology observation, histological observation and mechanical test were performed. Results: the repairing effect of osteochondral defect in the low stent group was better than that in the elevated stent group by the comprehensive observation and evaluation of the structure and function. The articular surface repaired by low scaffolds is flatter, the subchondral bone plate grows thicker and the mechanical properties are better. Conclusion: the silk fibroin scaffolds lower than the subchondral plate can promote the repair of articular osteochondral defects, suggesting a new application of osteochondral scaffolds to a certain extent.
【作者單位】： 北京體育大學(xué)運(yùn)動(dòng)康復(fù)系;浙江體育職業(yè)技術(shù)學(xué)院;浙江大學(xué);
【分類號(hào)】：R873;G804.2
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本文編號(hào)：2445403