【摘要】：目的:建立同步檢測雙側(cè)三叉神經(jīng)運動核(trigeminal motor nucleus,Mo5)電活動和咬肌電活動同步記錄技術(shù),為進一步分析咬肌H反射的神經(jīng)通路和可塑性機制奠定基礎。方法:取成年Wistar大鼠5只,在麻醉狀態(tài)下進行雙側(cè)Mo5區(qū)電活動的同步記錄,同時監(jiān)測雙側(cè)咬肌肌電(EMG)等多項生理指標,并對給予咬肌神經(jīng)電刺激時同步記錄到的肌電反應進行分析。結(jié)果:1記錄穩(wěn)定信號30min,可見Mo5區(qū)神經(jīng)元呈一低頻放電模式:左側(cè)(1.25±0.09)Hz,右側(cè)(1.20±0.39)Hz,P0.05,咬肌未見可測的電活動;2給予單側(cè)咬肌神經(jīng)電刺激(0.1~20mA,200μs,0.3Hz)不僅誘導出同側(cè)咬肌H反射,也誘導出對側(cè)咬肌H反射;3給予單側(cè)咬肌神經(jīng)電刺激誘發(fā)H反射時,同時引發(fā)同側(cè)和對側(cè)Mo5核團生物電反應;4刺激側(cè)咬肌EMG幅值明顯高于對側(cè)(P0.05);刺激側(cè)Mo5核團生物電幅值與對側(cè)相比較差異無統(tǒng)計學意義。結(jié)論:對大鼠咬肌H反射的雙側(cè)Mo5區(qū)和咬肌電活動可進行有效的同步記錄和分析,可用于咬肌H反射的神經(jīng)通路和可塑性機制研究。
[Abstract]:Aim: to establish a synchronous recording technique for detecting the electrical activity of bilateral trigeminal motor nucleus (trigeminal motor nucleus,Mo5) and masseter muscle activity synchronously, so as to lay a foundation for further analysis of the nerve pathway and plasticity mechanism of masseter H reflex. Methods: the electrical activity of bilateral Mo5 was recorded in 5 adult Wistar rats under anesthesia, and several physiological indexes, such as bilateral masseter electromyography (EMG), were monitored at the same time. The electromyoelectric responses recorded during nerve stimulation of masseter muscle were analyzed. Results: (1) after recording the stable signal for 30 minutes, the neurons in the Mo5 region showed a low frequency discharge pattern: the left side (1.25 鹵0.09) Hz, and the right side (1.20 鹵0.39) Hz,P0.05, had no measurable electrical activity. 2Induction of unilateral masseter nerve electrical stimulation (0.1 ~ 20mAn 200 渭 s ~ (0.3 Hz) not only induced ipsilateral masseter H reflex, but also induced contralateral masseter H reflex; 3When unilateral masseter nerve stimulation was given to induce H reflex, both ipsilateral and contralateral Mo5 nuclei were induced bioelectric response, 4the amplitude of EMG in stimulating side masseter muscle was significantly higher than that in contralateral side (P0.05). There was no significant difference in bioelectric amplitude between the stimulation side and the contralateral side. Conclusion: the simultaneous recording and analysis of bilateral Mo5 region and masseter electrical activity of masseter H reflex in rats can be used to study the neural pathway and plasticity mechanism of masseter H reflex.
【作者單位】： 皖南醫(yī)學院細胞電生理研究室;皖南醫(yī)學院口腔醫(yī)學院;
【基金】：國家自然科學基金(31271155) 皖南醫(yī)學院重點科研項目培育基金(wk2013Z09) 蕪湖市科技計劃項目(2012cxy16)
【分類號】：R338

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