鐵攝入過(guò)量對(duì)雄鼠組織損傷及雌鼠胚胎發(fā)育影響的實(shí)驗(yàn)研究
發(fā)布時(shí)間：2019-01-12 16:29
【摘要】：目的鐵是人體必需的微量元素之一,但攝入過(guò)多可使機(jī)體氧化應(yīng)激水平增強(qiáng),造成一系列病理變化或損傷。本實(shí)驗(yàn)以大鼠為研究對(duì)象,探索鐵攝入過(guò)量對(duì)組織損傷及胚胎發(fā)育的影響。方法雄性Wistar大鼠50只,隨機(jī)分為5組并分別飼喂含不同劑量鐵的飼料,貧鐵組(X1組)：每日鐵攝入量為1.67mg/kg體重,正常對(duì)照組(X2組)：每日鐵攝入量為7mg/kg體重,低劑量組(X3組)：每日鐵攝入量為21mg/kg體重,中劑量組(X4組)：每日鐵攝入量為63mg/kg體重,高劑量組(X5組)：每日鐵攝入量為189mg/kg體重。自由飲水,連續(xù)干預(yù)12周后處死,腹主動(dòng)脈取血,用全自動(dòng)生化儀檢測(cè)血漿總蛋白(TP)、白蛋白(ALB)、球蛋白(GLB)、白/球比(A/G)、谷丙轉(zhuǎn)氨酶(ALT)、谷草轉(zhuǎn)氨酶(AST)、尿素氮(BUN)、肌酐(CREA)、血紅蛋白濃度等各項(xiàng)生化指標(biāo)；剖取肝、腦、腎等臟器,檢測(cè)血漿鐵含量、肝、腦、腎鐵含量等。雌性Wistar大鼠60只,隨機(jī)分為5組：貧鐵組(C1組)、正常對(duì)照組(C2組)、低劑量組(C3組)、中劑量組(C4組)、高劑量組(C5組),與雄鼠各組干預(yù)措施相同,6周后,與雄鼠合籠交配。懷孕雌鼠妊娠第20天稱重,麻醉、剖取胎鼠,檢查胎鼠的死胎數(shù)、吸收胎數(shù)、外觀畸形數(shù)、著床數(shù)、體長(zhǎng)、尾長(zhǎng)等,制作胎鼠內(nèi)臟和骨骼標(biāo)本,檢查內(nèi)臟與骨骼的情況。結(jié)果隨鐵攝入劑量的升高,雄鼠血漿鐵含量、血紅蛋白濃度顯著提高：肝臟鐵含量、谷丙轉(zhuǎn)氨酶、谷草轉(zhuǎn)氨酶活性顯著提高,血漿總蛋白、球蛋白含量顯著降低；腎臟鐵含量、血漿尿素氮、肌酐含量隨著鐵攝入劑量的增高而顯著升高；各劑量組腦鐵含量無(wú)顯著差異。胎鼠出生情況檢查顯示,不同鐵劑量組胎鼠體表顏色隨鐵劑量增加而逐漸變深,貧鐵組胎鼠顏色較蒼白,正常對(duì)照組淡紅(正常),低劑量組較紅潤(rùn)、中劑量組深紅、高劑量組紫紅；生長(zhǎng)情況分析發(fā)現(xiàn)三個(gè)劑量組胎鼠的胎重、體長(zhǎng)、尾長(zhǎng)和胎盤重量隨鐵劑量的增加而下降；與正常對(duì)照組比較,胎重分別降低了13.3%、16.7%和17.2%(P值均0.05)；體長(zhǎng)分別減少了3.8%、9.7%和10.0%,(P值均0.05)；尾長(zhǎng)分別降低了3.6%、7.9%和5.0%(P值均0.05)；胎盤重分別降低了21.2%、23.1%和26.9%,(P值均0.05)；同時(shí)發(fā)現(xiàn),低、中、高劑量組胎鼠與正常對(duì)照組比較,吸收胎數(shù)、椎骨色黑率、內(nèi)臟出血率增加,中劑量組和高劑量組吸收胎數(shù)增加顯著(P0.05),分別升高了5.88%和5.97%;椎骨色黑率增加顯著(P0.05),分別升高了12.7%和12.9%;內(nèi)臟出血率三個(gè)劑量組均顯著增加(P0.05),分別提高了16%、27%和39%,隨鐵攝入量的增加而升高。結(jié)論雄性大鼠攝入過(guò)量的鐵可使機(jī)體多組織、臟器鐵含量提高,造成組織器官的損傷,使機(jī)體正常生理狀況發(fā)生紊亂；雌性大鼠攝入過(guò)量鐵可增加死胎、吸收胎甚至胚胎畸形的機(jī)率,導(dǎo)致胚胎發(fā)育毒性。因此鐵過(guò)量的危害應(yīng)引起關(guān)注。
[Abstract]:Objective Iron is one of the essential trace elements in human body, but excessive intake of iron can increase the level of oxidative stress and cause a series of pathological changes or injuries. In this study, the effects of excessive iron intake on tissue damage and embryonic development in rats were investigated. Methods 50 male Wistar rats were randomly divided into 5 groups and fed with different doses of iron. Iron deficiency group (X1 group): daily iron intake was 1.67mg/kg body weight, normal control group (X2 group): daily iron intake was 7mg/kg body weight. Low dose group (X3 group): daily iron intake was 21mg/kg body weight, medium dose group (X4 group): daily iron intake was 63mg/kg body weight, high dose group (X5 group): daily iron intake was 189mg/kg body weight. After 12 weeks of continuous intervention, blood was collected from abdominal aorta. Plasma total protein (TP), albumin (ALB), globulin (GLB), white / ball ratio (A / G) and alanine aminotransferase (ALT),) were detected by automatic biochemical instrument. Aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (CREA), hemoglobin concentration and other biochemical indicators; Liver, brain, kidney and other organs were dissected and plasma iron content, liver, brain and kidney iron content were measured. 60 female Wistar rats were randomly divided into 5 groups: iron deficient group (C1 group), normal control group (C2 group), low dose group (C3 group), middle dose group (C4 group) and high dose group (C5 group). Mate with male rat cage. On the 20th day of pregnancy, the female mice were weighed, anesthetized, the fetal rats were dissected, the number of dead fetus, the number of absorbed embryos, the number of appearance deformities, the number of implantation, the length of body, the length of tail and so on were examined. The viscera and bone specimens were made and the viscera and bone were examined. Results with the increase of iron intake dose, the plasma iron content and hemoglobin concentration of male rats were significantly increased: liver iron content, activity of alanine aminotransferase, glutamic oxaloacetic transaminase, plasma total protein and globulin decreased significantly. The contents of renal iron, plasma urea nitrogen and creatinine increased significantly with the increase of iron intake dose, but there was no significant difference in brain iron content among different dose groups. Birth examination of fetal mice showed that the body surface color of fetal mice in different iron dosage groups gradually became darker with the increase of iron dosage, the color of iron deficient group was paler, that of normal control group was light red (normal), that of low dose group was red and that of medium dose group was deep red, and that of iron poor group was lower than that of low dose group, and that of middle dose group was deep red. High dose group; Growth analysis showed that the fetal weight, body length, tail length and placental weight of the three dose groups decreased with the increase of iron dose. Compared with the normal control group, the fetal weight decreased by 13.3% and 17.2% (P < 0.05), the body length decreased by 3.8% and 10.0%, respectively (P < 0.05). The tail length was decreased by 3. 6% and 5. 0% (P < 0. 05), the placenta weight was decreased by 21. 2% and 26. 9% (P < 0. 05). At the same time, compared with the normal control group, the number of absorbed fetus, the rate of vertebral color and black, the rate of visceral hemorrhage in the low, middle and high dose groups increased significantly (P0.05). Increased 5.88% and 5.97% respectively; The rate of vertebral color and black increased significantly (P0.05), increased by 12.7% and 12.9%, and the rate of visceral hemorrhage increased significantly (P0.05), increased by 16% and 39%, respectively, and increased with the increase of iron intake. Conclusion excessive intake of iron in male rats can increase the content of iron in organs and tissues, and lead to injury of tissues and organs and disorder of normal physiological state of the body. Excessive iron intake in female rats can increase the chance of fetal death, absorption and even embryo malformation, leading to embryo development toxicity. Therefore, the harm of iron overdose should be concerned.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R153.1
本文編號(hào)：2407981
[Abstract]:Objective Iron is one of the essential trace elements in human body, but excessive intake of iron can increase the level of oxidative stress and cause a series of pathological changes or injuries. In this study, the effects of excessive iron intake on tissue damage and embryonic development in rats were investigated. Methods 50 male Wistar rats were randomly divided into 5 groups and fed with different doses of iron. Iron deficiency group (X1 group): daily iron intake was 1.67mg/kg body weight, normal control group (X2 group): daily iron intake was 7mg/kg body weight. Low dose group (X3 group): daily iron intake was 21mg/kg body weight, medium dose group (X4 group): daily iron intake was 63mg/kg body weight, high dose group (X5 group): daily iron intake was 189mg/kg body weight. After 12 weeks of continuous intervention, blood was collected from abdominal aorta. Plasma total protein (TP), albumin (ALB), globulin (GLB), white / ball ratio (A / G) and alanine aminotransferase (ALT),) were detected by automatic biochemical instrument. Aspartate aminotransferase (AST), urea nitrogen (BUN), creatinine (CREA), hemoglobin concentration and other biochemical indicators; Liver, brain, kidney and other organs were dissected and plasma iron content, liver, brain and kidney iron content were measured. 60 female Wistar rats were randomly divided into 5 groups: iron deficient group (C1 group), normal control group (C2 group), low dose group (C3 group), middle dose group (C4 group) and high dose group (C5 group). Mate with male rat cage. On the 20th day of pregnancy, the female mice were weighed, anesthetized, the fetal rats were dissected, the number of dead fetus, the number of absorbed embryos, the number of appearance deformities, the number of implantation, the length of body, the length of tail and so on were examined. The viscera and bone specimens were made and the viscera and bone were examined. Results with the increase of iron intake dose, the plasma iron content and hemoglobin concentration of male rats were significantly increased: liver iron content, activity of alanine aminotransferase, glutamic oxaloacetic transaminase, plasma total protein and globulin decreased significantly. The contents of renal iron, plasma urea nitrogen and creatinine increased significantly with the increase of iron intake dose, but there was no significant difference in brain iron content among different dose groups. Birth examination of fetal mice showed that the body surface color of fetal mice in different iron dosage groups gradually became darker with the increase of iron dosage, the color of iron deficient group was paler, that of normal control group was light red (normal), that of low dose group was red and that of medium dose group was deep red, and that of iron poor group was lower than that of low dose group, and that of middle dose group was deep red. High dose group; Growth analysis showed that the fetal weight, body length, tail length and placental weight of the three dose groups decreased with the increase of iron dose. Compared with the normal control group, the fetal weight decreased by 13.3% and 17.2% (P < 0.05), the body length decreased by 3.8% and 10.0%, respectively (P < 0.05). The tail length was decreased by 3. 6% and 5. 0% (P < 0. 05), the placenta weight was decreased by 21. 2% and 26. 9% (P < 0. 05). At the same time, compared with the normal control group, the number of absorbed fetus, the rate of vertebral color and black, the rate of visceral hemorrhage in the low, middle and high dose groups increased significantly (P0.05). Increased 5.88% and 5.97% respectively; The rate of vertebral color and black increased significantly (P0.05), increased by 12.7% and 12.9%, and the rate of visceral hemorrhage increased significantly (P0.05), increased by 16% and 39%, respectively, and increased with the increase of iron intake. Conclusion excessive intake of iron in male rats can increase the content of iron in organs and tissues, and lead to injury of tissues and organs and disorder of normal physiological state of the body. Excessive iron intake in female rats can increase the chance of fetal death, absorption and even embryo malformation, leading to embryo development toxicity. Therefore, the harm of iron overdose should be concerned.
【學(xué)位授予單位】：青島大學(xué)
【學(xué)位級(jí)別】：碩士
【學(xué)位授予年份】：2014
【分類號(hào)】：R153.1
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