【摘要】：目的觀察母兔球海綿體括約肌反射(BCR)的波形,并分析其在急性骶髓缺血后的變化特點。方法18只母兔隨機分為3組,預實驗組(n=6)用于確定最佳刺激強度及排除麻醉和手術對BCR的影響;對照組手術分離腰動脈而不結扎腰動脈,實驗組(n=6)結扎左腎動脈和動脈分叉間5條腰動脈建立骶髓缺血損傷動物模型,腰動脈結扎后連續(xù)記錄BCR波形2 h,觀察BCR波在急性骶髓缺血后的變化特點,分別記錄腰動脈結扎后2 h和2 d的BCR波形,2 d后取骶髓HE染色鏡下觀察。結果對照組和實驗組手術前均記錄到穩(wěn)定的BCR波,潛伏期(11.5±1.5)ms,波幅(261.2±275.3)μV;實驗組骶髓缺血后可見波幅即刻開始降低、潛伏期即刻開始延長,經(jīng)(0.67±0.40)min后波幅完全消失,實驗組手術后2 d波幅與基線的比值是0,低于對照組(99.2%±3.8%)(P0.05)。結論母兔可以記錄到穩(wěn)定的BCR波形,急性骶髓缺血后BCR波幅降低、潛伏期延長。
[Abstract]:Objective to observe the waveform of sphincter reflex (BCR) in female rabbits and to analyze its changes after acute sacral spinal cord ischemia. Methods Eighteen female rabbits were randomly divided into 3 groups. The experimental group (NN6) was used to determine the optimal stimulation intensity and to exclude the effect of anesthesia and operation on BCR. In the control group, the lumbar artery was separated without ligation of the lumbar artery. In the experimental group (NN6), 5 lumbar arteries were ligated between the left renal artery and the artery bifurcation to establish the animal model of sacral spinal cord ischemia injury. The BCR waveform was recorded continuously for 2 h after ligation of the lumbar artery. The changes of BCR wave after acute sacral spinal cord ischemia were observed. The BCR waveforms were recorded at 2 hours and 2 days after ligation of lumbar artery respectively. The sacral spinal cord was stained with HE after 2 days. Results the stable BCR wave was recorded in the control group and the experimental group before operation, the latency was (11.5 鹵1.5) ms, and the amplitude was (261.2 鹵275.3) 渭 V. After sacral spinal cord ischemia, the amplitude of sacral spinal cord in the experimental group began to decrease and the incubation period was prolonged immediately. After (0.67 鹵0.40) min, the amplitude of the wave disappeared completely. The ratio of amplitude to baseline was 0 on the 2nd day after operation in the experimental group. Lower than the control group (99.2% 鹵3.8%) (P0.05). Conclusion stable BCR waveforms can be recorded in female rabbits. The amplitude of BCR is decreased and the latency is prolonged after acute sacral spinal cord ischemia.
【作者單位】： 臨沂市第三人民醫(yī)院;
【基金】：山東醫(yī)藥衛(wèi)生科技發(fā)展計劃面上項目(2015WS0375)
【分類號】：R741

【參考文獻】

相關期刊論文 前2條

1 宋啟民;費昶;楊衛(wèi)忠;陳春美;;不完全性脊髓缺血損傷動物模型的建立及價值[J];中華神經(jīng)醫(yī)學雜志;2011年10期

2 朱廣友;鄭崇達;沈彥;王少華;;陰部神經(jīng)誘發(fā)電位研究[J];法醫(yī)學雜志;1993年03期

【共引文獻】

相關期刊論文 前3條

1 楊汶川;劉學鍵;邰茂眾;武宜雷;陳香利;;母兔球海綿體括約肌反射波形及其在急性骶髓缺血后的變化[J];山東醫(yī)藥;2017年29期

2 張俐;安國堯;陳凱;李長征;張文光;;夾閉腹主動脈和腰動脈的脊髓缺血再灌注損傷模型比較研究[J];醫(yī)學研究生學報;2014年02期

3 朱廣友;候心一;沈彥;陳梅英;王少華;;50例男子性功能鑒定分析[J];法醫(yī)學雜志;1999年03期

【二級參考文獻】

相關期刊論文 前3條

1 宋啟民;楊衛(wèi)忠;陳春美;;兔子脊髓缺血動物模型建立的解剖學基礎[J];中國神經(jīng)精神疾病雜志;2010年07期

2 楊衛(wèi)忠;宋啟民;陳春美;石松生;王春華;賈建文;房新蓉;涂獻坤;;皮層體感誘發(fā)電位在不同水平脊髓缺血中的變化[J];中華神經(jīng)醫(yī)學雜志;2010年05期

3 鄭菊陽;徐迎勝;張朔;張俊;康德tD;樊東升;;接觸性熱痛誘發(fā)電位的臨床研究[J];中華神經(jīng)醫(yī)學雜志;2009年07期

，

本文編號：2406843